Research Spotlight - November 2021

Our November edition of Research Spotlight highlights 10 research studies that provide deeper insights on the biological functions of several key biomarkers and their clinical translational potential.

Two studies reveal new insights for diabetes treatment by investigating the functions of PGRMC1 and an m6A reader protein for insulin metabolism and delayed diabetic wound healing respectively.

Scientists also perform bioinformatics and immunological analysis of several regulatory pathways to examine new targets to combat several diseases, such as pneumonia, cancers, and atherosclerosis.

Scroll down to find out more about recent discoveries from fellow scientists!

PGRMC1 Acts as a Size-Selective Cargo Receptor to Drive ER-Phagic Clearance of Mutant Prohormones

Authors: Chen, Y.J., Knupp, J., Arunagiri, A., Haataja, L., Arvan, P., Tsai, B.
Journal: Nature Communications

The reticulon-3 (RTN3)-driven targeting complex promotes clearance of misfolded prohormones from the endoplasmic reticulum (ER) for lysosomal destruction by ER-phagy. Because RTN3 resides in the cytosolic leaflet of the ER bilayer, the mechanism of selecting misfolded prohormones as ER-phagy cargo on the luminal side of the ER...

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Cited Boster Product(s): Anti-Reticulon-3 RTN3 Antibody (PA2256)

Lung-Targeting Lysostaphin Microspheres for Methicillin-Resistant Staphylococcus aureus Pneumonia Treatment and Prevention

Authors: Lin, X., He, J., Li, W., Qi, Y., Hu, H., Zhang, D., Xu, F., Chen, X...
Journal: ACS Nano

Multifunctional antimicrobial strategies are urgently needed to treat methicillin-resistant Staphylococcus aureus (MRSA) caused pneumonia due to its increasing resistance, enhanced virulence, and high pathogenicity. Here, we report that lysostaphin, a bacteriolytic enzyme, encapsulated within poly(lactic-co-glycolic acid) microspheres...

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Cited Boster Product(s): Mouse IL-6/Interleukin-6 ELISA Kit PicoKine™ (EK0411); Mouse IL-1 Beta/IL-1F2/IL1B ELISA Kit PicoKine™ (EK0394); Mouse TNF Alpha/Tumor Necrosis Factor ELISA Kit PicoKine™ (EK0527)

m6A Reader YTHDC1 Modulates Autophagy by Targeting SQSTM1 in Diabetic Skin

Authors: Liang, D., Lin, W.J., Ren, M., Qiu, J., Yang, C., Wang, X., Li, N...
Journal: Autophagy

Dysregulation of macroautophagy/autophagy contributes to the delay of wound healing in diabetic skin. N6-methyladenosine (m6A) RNA modification is known to play a critical role in regulating autophagy. In this study, it was found that SQSTM1/p62 (sequestosome 1), an autophagy receptor, was significantly downregulated in two human...

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Cited Boster Product(s): Tris Buffered Saline (TBS) Powder (AR0031)

RNA-Binding Protein RBM24 Represses Colorectal Tumourigenesis by Stabilising PTEN mRNA

Authors: Xia, R.M., Liu, T., Li, W.G., Xu, X.Q.
Journal: Clinical and Translational Medicine

Background: RNA-binding motif protein 24 (RBM24) functions as a splicing regulator, which is critical for organ development and is dysregulated in human cancers. Here, we aim to uncover the biological function of RBM24 in colorectal tumourigenesis. Methods: Xenograft tumour model, Rbm24 knockout and Apcmin/+ mouse models were utilised...

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Cited Boster Product(s): HRP Conjugated AffiniPure Goat Anti-Mouse IgM (BA1075); HRP Conjugated AffiniPure Goat Anti-Rabbit IgG (H+L) (BA1054)

Nuclear TIGAR mediates an epigenetic and metabolic autoregulatory loop via NRF2 in cancer therapeutic resistance

Authors: Wang, H., Wang, Q., Cai, G., Duan, Z., Nugent, Z., Huang, J...
Journal: Acta Pharmaceutica Sinica B

Metabolic and epigenetic reprogramming play important roles in cancer therapeutic resistance. However, their interplays are poorly understood. We report here that elevated TIGAR (TP53-induced glycolysis and apoptosis regulator), an antioxidant and glucose metabolic regulator and a target of oncogenic histone methyltransferase NSD2...

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Cited Boster Product(s): Anti-MAFF Antibody Picoband™ (A07784-2); Anti-MAFG Antibody Picoband™ (A04636-2)

Targeting MUS81 Promotes the Anticancer Effect of WEE1 Inhibitor and Immune Checkpoint Blocking Combination Therapy via Activating cGAS/STING Signaling in Gastric Cancer Cells

Authors: Li, C., Shen, Q., Zhang, P., Wang, T., Liu, W., Li, R., Ma, X., Zeng, X...
Journal: Journal of Experimental & Clinical Cancer Research

Background: Identification of genomic biomarkers to predict the anticancer effects of indicated drugs is considered a promising strategy for the development of precision medicine. DNA endonuclease MUS81 plays a pivotal role in various biological processes during malignant diseases, mainly in DNA damage repair and replication fork...

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Cited Boster Product(s): Anti-Ki67/Mki67 Antibody Picoband™ (A00254); Alkaline Phosphatase Conjugated Rabbit Anti-Human IgG SABC Kit (SA1054)

Prenatal Exposure to Environmentally Relevant Levels of PBDE-99 Leads to Testicular Dysgenesis with Steroidogenesis Disorders

Authors: Zhao, T., Tang, X., Li, D., Zhao, J., Zhou, R., Shu, F., Jia, W., Fu, W...
Journal: Journal of Hazardous Materials

Polybrominated diphenyl ethers (PBDEs) are a widely used class of brominated flame retardants. Exposure to PBDEs could induce testicular damage in mammals, but the effects and potential mechanism of action of prenatal exposure to environmentally relevant PBDEs on testicular development remain unclear. For the in vivo study, pregnant...

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Cited Boster Product(s): Anti-Bax Antibody Picoband™ (A00183); Anti-GAPDH Antibody Picoband™ (A00227)

An Atherosclerotic Plaque-Targeted Single-Chain Antibody for MR/NIR-II Imaging of Atherosclerosis and Anti-Atherosclerosis Therapy

Authors: Zhang, L., Xue, S., Ren, F., Huang, S., Zhou, R., Wang, Y., Zhou, C...
Journal: Journal of Nanobiotechnology

Background: Oxidation-specific epitopes (OSEs) are rich in atherosclerotic plaques. Innate and adaptive immune responses to OSEs play an important role in atherosclerosis. The purpose of this study was to develop novel human single-chain variable fragment (scFv) antibody specific to OSEs to image and inhibit atherosclerosis...

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Cited Boster Product(s): Mouse CRP / C Reactive Protein / PTX1 ELISA Kit PicoKine™ (EK0977); Mouse IL-6/Interleukin-6 ELISA Kit PicoKine™ (EK0411); Mouse TNF Alpha/Tumor Necrosis Factor ELISA Kit PicoKine™ (EK0527)

In Situ Prevascularization Strategy with Three-Dimensional Porous Conduits for Neural Tissue Engineering

Authors: Shen, J., Wang, J., Liu, X., Sun, Y., Yin, A., Chai, Y., Zhang, K…
Journal: ACS Applied Materials & Interfaces

Neovascularization is crucial for peripheral nerve regeneration and long-term functional restoration. Previous studies have emphasized strategies that enhance axonal repair over vascularization. Here, we describe the development and application of an in situ prevascularization strategy that uses 3D porous nerve guidance conduits...

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Cited Boster Product(s): Human VEGF ELISA Kit PicoKine™ (EK0539)

Diet-Induced Obesity Accelerates Oral Carcinogenesis by Recruitment and Functional Enhancement of Myeloid-Derived Suppressor Cells

Authors: Peng, J., Hu, Q., Chen, X., Wang, C., Zhang, J., Ren, X., Wang, Y…
Journal: Cell Death & Disease

Although obesity has been associated with an increased risk and aggressiveness of many types of carcinoma, whether it promotes squamous cell carcinoma remains unclear. To reveal the role of obesity in oral squamous cell carcinoma (OSCC) initiation and development, we used 4NQO-induced OSCC model mice to examine the impact of dietary...

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Cited Boster Product(s): Mouse CCL9/MIP-1 Gamma ELISA Kit PicoKine™ (EK1225)