Research Spotlight - November 2020

In our November edition of Research Spotlight, we feature 10 recently published research papers from scientists whom Boster has served.

This month’s selected research publications investigate how several cells and mediators regulate the development of fibrotic diseases, cancer, inflammatory responses, diabetes, allergy, endometriosis, and anxiety.

Among the studies, scientists propose the underlying mechanisms of how endothelin receptor antagonists may serve as a therapeutic strategy for fibrotic diseases. The role of PRMT5 in laryngeal tumor carcinogenesis is also explored.

Discover more details about these new scientific discoveries below.

Second-Strand Synthesis-Based Massively Parallel scRNA-Seq Reveals Cellular States and Molecular Features of Human Inflammatory Skin Pathologies

Authors: Hughes, T.K., Wadsworth, M.H., Gierahn, T.M., Do, T., Weiss, D...
Journal: Immunity

High-throughput single-cell RNA-sequencing (scRNA-seq) methodologies enable characterization of complex biological samples by increasing the number of cells that can be profiled contemporaneously. Nevertheless, these approaches recover less information per cell than low-throughput strategies. To accurately report the expression of...

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Cited Boster Product(s): Anti-Fra-1 (D134) FOSL1 Antibody (A03927)

CD1d1 Intrinsic Signaling in Macrophages Controls NLRP3 Inflammasome Expression During Inflammation

Authors: Cui, S., Wang, C., Bai, W., Li, J., Pan, Y., Huang, X., Yang, H., Feng, Z...
Journal: Science Advances

Dysregulation of immune responses in the gut often associates with inflammatory bowel diseases (IBD). Mouse CD1d1, an ortholog of human CD1d mainly participating in lipid-antigen presentation to NKT cells, is able to generate intrinsic signals upon stimulation. Mice with macrophage-specific CD1d1 deficiency (LymCD1d1−/−) acquire...

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Cited Boster Product(s): Mouse IL-1 Beta ELISA Kit PicoKine™ (EK0394); Mouse GM-CSF ELISA Kit PicoKine™ (EK0365)

SMAD3 Promotes Autophagy Dysregulation by Triggering Lysosome Depletion in Tubular Epithelial Cells in Diabetic Nephropathy

Authors: Yang, C., Chen, X.C., Li, Z.H., Wu, H.L., Jing, K.P., Huang, X.R., Ye, L...
Journal: Autophagy

Macroautophagy/autophagy dysregulation has been noted in diabetic nephropathy; however, the regulatory mechanisms controlling this process remain unclear. In this study, we showed that SMAD3 (SMAD family member 3), the key effector of TGFB (transforming growth factor beta)-SMAD signaling, induces lysosome depletion via the inhibition...

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Cited Boster Product(s): Phosphate Buffered Saline (PBS) Powder (AR0030)

Butyrate as Bioactive Human Milk Protective Component Against Food Allergy

Authors: Paparo, L., Nocerino, R., Ciaglia, E., Di Scala, C., De Caro, C., Russo, R...
Journal: Allergy

Background: Food allergy (FA) is a growing health problem worldwide. Effective strategies are advocated to limit the disease burden. Human milk (HM) is considered a protective factor for FA, but its mechanisms remain unclear. Butyrate is a gut microbiota‐derived metabolite able to exert several immunomodulatory functions...

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Cited Boster Product(s): Human IL-4 ELISA Kit PicoKine™ (EK0404); Human IL-10 ELISA Kit PicoKine™ (EK0416)

BPA Modulates the WDR5/TET2 Complex to Regulate ERβ Expression in Eutopic Endometrium and Drives the Development of Endometriosis

Authors: Xue, W., Yao, X., Ting, G., Ling, J., Huimin, L., Yuan, Q., Chun, Z., Ming, Z...
Journal: Environmental Pollution

Overexpression of estrogen receptor β (ERβ) in endometrium contributes to endometriosis (EM) pathogenesis. Trimethylation of the H3 lysine (K) 4 (H3K4me3) in promoters is strongly correlated with gene expression. This study aimed to explore the effects of bisphenol A (BPA) exposure on EM development from the perspective of the...

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Cited Boster Product(s): Goat Anti-Rabbit IgG (H+L) Secondary Antibody, FITC Conjugated (BA1105); Goat Anti-Mouse IgG (H+L) Secondary Antibody, Cy3 Conjugated (BA1031)

Endothelin Receptors Promote Schistosomiasis-Induced Hepatic Fibrosis via Splenic B Cells

Authors: Kong, H., He, J., Guo S., Song, Q., Xiang, D., Tao, R., Yu, H., Chen. G...
Journal: PLoS Pathogens

Endothelin receptors (ETRs) are activated by vasoactive peptide endothelins and involved in the pathogenesis of hepatic fibrosis. However, less is known about the role of ETRs in Schistosoma (S.) japonicum-induced hepatic fibrosis. Here, we show that the expression of ETRs is markedly enhanced in the liver and spleen tissues...

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Cited Boster Product(s): Broad Spectrum Protease Inhibitor Cocktail (100x) (AR1182); Mouse IL-10 ELISA Kit PicoKine™ (EK0417); Mouse TGF Beta 1 ELISA Kit PicoKine™ (EK0515)

PRMT5/Wnt4 Axis Promotes Lymph-Node Metastasis and Proliferation of Laryngeal Carcinoma

Authors: Wang, N., Yan, H., Wu, D., Zhao, Z., Chen, X., Long, Q., Zhang, C...
Journal: Cell Death & Disease

Metastasis is the main cause of laryngeal cancer-related death; its molecular mechanism remains unknown. Here we identify protein arginine methyltransferase 5 (PRMT5) as a new metastasis-promoting factor in laryngeal carcinoma, and explore its underlying mechanism of action in regulating laryngeal cancer progression. We illustrated...

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Cited Boster Product(s): Anti-Human Ki-67 Monoclonal Antibody (M00254-1); Anti-Lamin B1/LMNB1 Antibody Picoband™ (PB9611)

Tak1 in the Astrocytes of Mediobasal Hypothalamus Regulates Anxiety‐Like Behavior in Mice

Authors: Zhao, F., Zhang, T., Shen, Q., Yin, K., Wang, Y., & Zhang, G.
Journal: GLIA

Anxiety disorder is characterized by excessive fear, anxiety, and avoidance of perceived threats in internal to oneself or the environment, however, the underlying mechanisms are less well understood. Here, we show that transforming growth factor‐β‐activated kinase 1 (Tak1) expressed in the astrocytes of mediobasal hypothalamus (MBH)...

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Cited Boster Product(s): Anti-GFAP Antibody Picoband™ (PB9082)

Sub-Chronic Microcystin-LR Renal Toxicity in Rats Fed a High Fat/High Cholesterol Diet

Authors: Arman, T., Lynch, K.D., Goedken, M., & Clarke, J.D.
Journal: Chemosphere

Microcystin-LR (MCLR) is a liver and kidney toxin produced by cyanobacteria. Recently, it was demonstrated that MCLR exposure drives the progression of high fat/high cholesterol (HFHC) induced nonalcoholic fatty liver disease (NAFLD) to a more severe state. NAFLD is also a risk factor for chronic kidney disease (CKD), and the current...

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Cited Boster Product(s): Rat KIM1 ELISA Kit PicoKine™ (EK0882)

The Role of Nrf2/PIWIL2/purine Metabolism Axis in Controlling Radiation-Induced Lung Fibrosis

Authors: Zhou, G.L., Zhang, X.R., Ma, Y.L., Lu, Q., Zhao, R., Zhu, Y.Z., & Wang, Y.Y.
Journal: American Journal of Cancer Research

NF-E2-related factor 2 (Nrf2) is a key transcription factor recently implicated in the control of radiation-induced lung fibrosis (RILF). However, the molecular mechanism of Nrf2 in the pathogenesis of RILF is still unclear. The purpose of this study was to evaluate the regulatory effect and mechanism of Nrf2 in the pathogenesis of...

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Cited Boster Product(s): Human TGF Beta 1 ELISA Kit PicoKine™ (EK0513)