Research Spotlight - March 2023

Welcome to the March issue of Research Spotlight! This month, we present an array of research papers offering new insights for cutting-edge biomedical technologies.

One research study describes a novel nanostructure with magnetocaloric oscillation and catalytic effects for pyroptosis-triggered tumor immunotherapy. Another study investigates a brain-targeted peptide-functionalized chitosan nanoparticle for resveratrol delivery, showing promise in combating obesity-related Alzheimer's disease.

Additionally, featured papers contain topics on Litchi procyanidins that inhibit colon cancer proliferation and metastasis, a 3D bioprinting technique for cartilage regeneration, and a novel implant surface modification mode for osteoblastogenesis and angiogenesis.

Scroll down to find out more about these new discoveries!

Structure and Functional Determinants of Rad6–Bre1 Subunits in the Histone H2B Ubiquitin-Conjugating Complex

Authors: Shukla, P.K., Bissell, J.E., Kumar, S., Pokhrel, S., Palani, S…
Journal: Nucleic Acids Research

The conserved complex of the Rad6 E2 ubiquitin-conjugating enzyme and the Bre1 E3 ubiquitin ligase catalyzes histone H2B monoubiquitination (H2Bub1), which regulates chromatin dynamics during transcription and other nuclear processes. Here, we report a crystal structure of Rad6 and the non-RING domain N-terminal region of Bre1...

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Cited Boster Product(s): Anti-Yeast UBC2 Antibody (DZ33919)

PtNi Nano Trilobal-Based Nanostructure with Magnetocaloric Oscillation and Catalytic Effects for Pyroptosis-Triggered Tumor Immunotherapy

Authors: Wang, S., Vong, L.B., Heger, Z., Zhou, Y., Liang, X., Adam, V., Li, N.
Journal: nanotoday

Pyroptosis, a unique form of programmed cell death, has been verified to be linked to inflammatory diseases and malignant tumors. Although great achievements have been made in pyroptosis research, several gaps, such as innate drug resistance and severe toxicity, hinder the development of pyroptosis inducers for biomedical applications...

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Cited Boster Product(s): Mouse IL-1 Beta/IL1B ELISA Kit PicoKine® (EK0394); Mouse TNF Alpha/Tumor Necrosis Factor ELISA Kit PicoKine® (EK0527)

IL15 Modification Enables CAR T Cells to Act as a Dual Targeting Agent Against Tumor Cells and Myeloid-Derived Suppressor Cells in GBM

Authors: Zannikou, M., Duffy, J.T., Levine, R.N., Seblani, M., Liu, Q., Presser, A…
Journal: Journal of ImmunoTherapy for Cancer

Introduction: The immunosuppressive tumor microenvironment (TME) is a major barrier to the efficacy of chimeric antigen receptor T cells (CAR-T cells) in glioblastoma (GBM). Transgenic expression of IL15 is one attractive strategy to modulate the TME. However, at present, it is unclear if IL15 could be used to directly target...

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Cited Boster Product(s): Anti-IL15RA Antibody Picoband™ (A03016-1)

Ultrasound Targeted Microbubble Destruction-Triggered Nitric Oxide Release via Nanoscale Ultrasound Contrast Agent for Sensitizing Chemoimmunotherapy

Authors: Zhao, Y., Shi, D., Guo, L., Shang, M., Sun, X., Meng, D., Xiao, S…
Journal: Journal of Nanobiotechnology

Immunotherapy had demonstrated inspiring effects in tumor treatment, but only a minority of people could benefit owing to the hypoxic and immune-suppressed tumor microenvironment (TME). Therefore, there was an urgent need for a strategy that could relieve hypoxia and increase infiltration of tumor lymphocytes simultaneously...

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Cited Boster Product(s): Mouse IFN Gamma ELISA Kit PicoKine® (EK0375); Mouse TNF Alpha ELISA Kit PicoKine® (EK0527)

A Novel Implant Surface Modification Mode of Fe3O4-Containing TiO2 Nanorods with Sinusoidal Electromagnetic Field for Osteoblastogenesis and Angiogenesis

Authors: Ren, R., Guo, J., Song, H., Wei, Y., Luo, C., Zhang, Y., Chen, L…
Journal: Materials Today Bio

Implants made of Ti and its alloys are widely utilized in orthopaedic surgeries. However, insufficient osseointegration of the implants often causes complications such as aseptic loosening. Our previous research discovered that disordered titanium dioxide nanorods (TNrs) had satisfactory antibacterial properties and biocompatibility...

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Cited Boster Product(s): 4% Paraformaldehyde (PFA) Solution In PBS (AR1068); DAPI for Nucleic Acid Staining (AR1176); Plus RIPA Lysis Buffer (AR0102-100); Broad Spectrum Protease Inhibitor Cocktail (100x) (AR1182); Anti-GAPDH Antibody Picoband™ (A00227-1)

Brain Targeted Peptide-Functionalized Chitosan Nanoparticles for Resveratrol Delivery: Impact on Insulin Resistance and Gut Microbiota in Obesity-Related Alzheimer's Disease

Authors: Yang, L., Wang, Y., Li, Z., Wu, X., Mei, J., Zheng, G.
Journal: Carbohydrate Polymers

The pathology of Alzheimer's disease (AD) is highly correlated with obesity-induced insulin resistance. Resveratrol (Res) is a natural phenol that demonstrates a neuroprotective effect, but the bioactivity of Res is low in vivo. Here, chitosan (CS) was cross-linked with sodium tripolyphosphate (TPP) to encapsulate low water solubility...

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Cited Boster Product(s): Mouse IL-6 ELISA Kit PicoKine® (EK0411); Mouse IL-1 Beta ELISA Kit PicoKine® (EK0394); Mouse TNF Alpha ELISA Kit PicoKine® (EK0527)

3D Bioprinting Using Synovium-Derived MSC-Laden Photo-Cross-Linked ECM Bioink for Cartilage Regeneration

Authors: Sang, S., Mao, X., Cao, Y., Liu, Z., Shen, Z., Li, M., Jia, W., Guo, Z…
Journal: ACS Applied Materials & Interfaces

In this study, inspired by the components of cartilage matrix, a photo-cross-linked extracellular matrix (ECM) bioink composed of modified proteins and polysaccharides was presented, including gelatin methacrylate, hyaluronic acid methacrylate, and chondroitin sulfate methacrylate. The systematic experiments were performed...

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Cited Boster Product(s): DAPI for Nucleic Acid Staining (AR1176); Goat Anti-Rabbit IgG (H+L) Secondary Antibody, DyLight®594 Conjugated (BA1142)

Litchi Procyanidins Inhibit Colon Cancer Proliferation and Metastasis by Triggering Gut-Lung Axis Immunotherapy

Authors: Yao, Y., Feng, S., Li, X., Liu, T., Ye, S., Ma, L., Man, S.
Journal: Cell Death & Disease

Litchi chinensis seed, as a valuable by-product of the subtropical fruit litchi (Litchi chinensis Sonn.), has been confirmed to be rich in procyanidins (LPC). The anticarcinogenic properties of procyanidins has been primarily attributed to their antioxidant and anti-inflammatory activities. However, there is a comparative paucity...

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Cited Boster Product(s): Anti-E Cadherin 1/CDH1 Antibody Picoband™ (PB9561); Anti-Vimentin Antibody Picoband™ (PB9359)

Enhanced Liquidity of p62 Droplets Mediated by Smurf1 Links Nrf2 Activation and Autophagy

Authors: Xia, Q., Li, Y., Xu, W., Wu, C., Zheng, H., Liu, L., Dong, L.
Journal: Cell & Bioscience

Background: Macro-autophagy/Autophagy is an evolutionarily well-conserved recycling process to maintain the balance through precise spatiotemporal regulation. However, the regulatory mechanisms of biomolecular condensates by the key adaptor protein p62 via liquid-liquid phase separation (LLPS) remain obscure. Results: In this study...

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Cited Boster Product(s): HRP Conjugated AffiniPure Goat Anti-Rabbit IgG (H+L) (BA1054); HRP Conjugated AffiniPure Goat Anti-Mouse IgG (H+L) (BA1050)

Transcription Factor c-Maf Deletion Improves Streptozotocin-Induced Diabetic Nephropathy by Directly Regulating Sglt2 and Glut2

Authors: Fujino, M., Morito, N., Hayashi, T., Ojima, M., Ishibashi, S., Kuno, A…
Journal: JCI Insight

The transcription factor c-Maf has been widely studied and has been reported to play a critical role in embryonic kidney development; however, the postnatal functions of c-Maf in adult kidneys remain unknown as c-Maf null C57BL/6J mice exhibit embryonic lethality. In this study, we investigated the role of c-Maf in adult mouse kidneys...

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Cited Boster Product(s): Anti-C-Maf Rabbit Monoclonal Antibody (M00654)