FACS Sorting Preparation Checklist

Are you preparing for a FACS experiment? Here’s a 10 point checklist to help you prepare for your FACS sorting experiment:

• Perform a cell viability count using the trypan blue exclusion method.
• Remove any existing clumps in the cell suspension by sieving cells through a nylon mesh.
• Adjust the cell concentration to 107-108 per ml.
• Ensure that all staining controls are included in the panel.
• Check that the correct nozzle is installed.
• Check that the sheath fluid levels are adequate.
• Ensure that the collection media had serum and HEPES buffer added to it.
• Check that the collection tubes are protein pre-coated ones.
• Make sure all the lasers and filters are functioning properly.
• Adjust the threshold level of the instrument as required.

For more resources, check out our Flow Cytometry Technical Resource Center!

*Note to educators: You are permitted to share Boster Bio's resources and PDFs on your class websites and lab websites. Please make sure to cite or link to the origin.

Boster Products for Flow Cytometry

Anti-Human/Mouse CD45R (B220) Ptprc Monoclonal Antibody PE Conjugated, Flow Validated

SKU	FC00555-2-PE
Reactivity	Human, Mouse
Applications	Flow Cytometry

Anti-Human CD3 Cd3e Monoclonal Antibody FITC Conjugated, Flow Validated

SKU	FC02675-FITC
Reactivity	Human
Applications	Flow Cytometry

Anti-Human CD19 Monoclonal Antibody PerCP-Cy5.5 Conjugated, Flow Validated

SKU	FC00154-PerCP-Cy5.5
Reactivity	Human
Applications	Flow Cytometry

Anti-Human CD3 Cd3e Monoclonal Antibody APC Conjugated, Flow Validated

SKU	FC02675-APC
Reactivity	Human
Applications	Flow Cytometry

Anti-Human CD45 PTPRC Monoclonal Antibody PerCP Conjugated, Flow Validated

SKU	FC00555-PerCP
Reactivity	Human
Applications	Flow Cytometry

Anti-Human CD71 TFRC Monoclonal Antibody Unconjugated, Flow Validated

SKU	FC00591
Reactivity	Human
Applications	Flow Cytometry

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All Boster antibodies and ELISA kits are guaranteed to meet the specifications on the data sheet. We promise to thoroughly investigate any concerns about the quality of our products; if you encounter a problem with a Boster antibody or ELISA kit, our technical support team will respond with personalized advice within 24 hours. If we can’t make your experiment work, we will refund your purchase in full or provide a replacement free of charge.

Product Review

Anti-GRP94 Antibody (PA1340)

Review : "This is an excellent antibody to endoplasmin in HC11 cells. Clean, reliable detection with very little if any background. Great antibody for Westerns. Could probably be optimized for 1 hour primary incubation."

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