Boster Bio Life Science Blog

In this article, you will find the materials needed to draw a standard curve, the process of drawing a standard curve, and how to utilize it.

Are you familiar with the multiple methods you could use to perform an ELISA? Among the standard assay formats illustrated below, where differences in both capture and detection are in concern, it is important to differentiate between the particular strategies that exist specifically for the detection step. However an antigen is captured to the

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ELISA (enzyme-linked immunosorbent assay) is a plate-based assay used to detect the concentration of a specific protein in a liquid sample. Three different types of data output can be obtained:

Types of Data Output

• Qualitative: ELISAs can be used to determine whether or not the protein of interest is present in the sample by comparing the sample to a blank well or an unrelated control antigen without the target protein.
• Semi-Quantitative: Since the signal intensity produced is directly related to the amount of target protein in the sample, ELISA allows samples to be compared by observing the relative levels of the target protein.
• Quantitative: Quantitative: For a precise calculation of the target protein concentration in assay samples, a standard curve with known target protein concentrations should be created using a purified antigen and compared with the ELISA data generated.

To set up a standard curve, ELISA standards should be carefully prepared for accurate sample quantification of...

ELISA (enzyme-linked immunosorbent assay) is a convenient and simple method to quantitatively or qualitatively detect peptides, proteins, antibodies, and hormones in samples, rendering it as one of the most widely used immunoassays. Despite the many advantages of conducting ELISA, there are some mistakes that could turn your ELISA experiment sour. Help prevent this situation from happening by avoiding 5 common pitfalls when performing an ELISA:

TMB Coloring Development Agent Dilemma

1. “I think I still have some of the TMB coloring development agent from the previous kit. Maybe I’ll use that instead.”

Stop! Wanting to conserve resources is a good habit, but not in this case. We should avoid using reagents from different batches together. Each...

The ELISA (enzyme-linked immunosorbent assay) is recognized by scientists for its many advantages. The assay is convenient, quick, and simple to execute. ELISA’s versatility to detect peptides, proteins, antibodies, and hormones, and its ability to generate quantitative and qualitative data make it one of the most popular and powerful immunoassays available.

In response to popular demand, numerous commercial ELISA kits are offered in the market, but not all ELISA kits are created equal.

How do we sift through the masses and choose a good ELISA kit?

Keep in mind the following points next time you go hunting for an ELISA kit.

Assay Format

• What would you like to determine for your sample? There are four categories of ELISAs - direct, indirect, sandwich, and competitive.
• If you’re planning for speedy detection of immune response to an antigen, and signal amplification isn’t a priority, direct ELISA might be for you.
• For better sensitivity and greater flexibility to figure out the total antibody concentration, indirect ELISA might be more suitable.
• What if your sample is complex? Fear not, choose sandwich ELISA for high sensitivity, specificity, and f
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Browse the promoted ELISA kits... and claim your discount. Take advantage of the promotion before it ends!

What is a Dilution Ratio?

Dilution ratio describes a simple dilution – a unit volume of solute (or sample) is combined with a desired unit volume of solvent (or diluent), to reach a desired total volume (Vsolute + Vsolvent = Total Vsolution)

Thus, a dilution ratio of 1:4 describes 1 part solute + 4 parts solvent = 5 parts total. The sum of both solute plus solvent equals total, f...