Product Info Summary
SKU: | A16546 |
---|---|
Size: | 100 µl |
Reactive Species: | Human |
Host: | Rabbit |
Application: | WB |
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Product info
Product Name
Anti-ZNF682/Zinc Finger Protein 682 Antibody
View all Zinc finger protein 682 Antibodies
SKU/Catalog Number
A16546
Size
100 µl
Form
Liquid
Description
Boster Bio Anti-ZNF682/Zinc Finger Protein 682 Antibody (Catalog # A16546). Tested in WB applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-ZNF682/Zinc Finger Protein 682 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A16546)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthesized peptide derived from internal of human ZNF682.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A16546 is reactive to ZNF682 in Human
Reconstitution
Calculated molecular weight
58361 MW
Background of Zinc finger protein 682
May be involved in transcriptional regulation.
Ota T., Nat. Genet. 36:40-45(2004).
Grimwood J., Nature 428:529-535(2004).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A16546 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blotting: 1:500~1:3000
Validation Images & Assay Conditions
Click image to see more details
Western blot analysis of extracts from COLO205 cells and HUVEC cells, using ZNF682 antibody A16546.
Protein Target Info & Infographic
Gene/Protein Information For ZNF682 (Source: Uniprot.org, NCBI)
Gene Name
ZNF682
Full Name
Zinc finger protein 682
Weight
58361 MW
Superfamily
krueppel C2H2-type zinc-finger protein family
Alternative Names
Zinc finger protein 682;ZNF682; ZNF682 BC39498_3 zinc finger protein 682 zinc finger protein 682
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on ZNF682, check out the ZNF682 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for ZNF682: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-ZNF682/Zinc Finger Protein 682 Antibody (A16546)
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No publications found for A16546
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Customer Reviews
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Customer Q&As
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6 Customer Q&As for Anti-ZNF682/Zinc Finger Protein 682 Antibody
Question
Please see the WB image, lot number and protocol we used for brain using anti-ZNF682/Zinc Finger Protein 682 antibody A16546. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2019-12-17
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-12-17
Question
Does anti-ZNF682/Zinc Finger Protein 682 antibody A16546 work on primate WB with oocyte?
Verified Customer
Verified customer
Asked: 2019-09-16
Answer
Our lab technicians have not validated anti-ZNF682/Zinc Finger Protein 682 antibody A16546 on primate. You can run a BLAST between primate and the immunogen sequence of anti-ZNF682/Zinc Finger Protein 682 antibody A16546 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated primate samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in primate oocyte in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-09-16
Question
Will A16546 anti-ZNF682/Zinc Finger Protein 682 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-07-04
Answer
As indicated on the product datasheet, A16546 anti-ZNF682/Zinc Finger Protein 682 antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-07-04
Question
I see that the anti-ZNF682/Zinc Finger Protein 682 antibody A16546 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-07-01
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2019-07-01
Question
We are currently using anti-ZNF682/Zinc Finger Protein 682 antibody A16546 for human tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human. Is it likely that the antibody can work on primate tissues as well?
Verified Customer
Verified customer
Asked: 2019-06-28
Answer
The anti-ZNF682/Zinc Finger Protein 682 antibody (A16546) has not been validated for cross reactivity specifically with primate tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in primate you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-06-28
Question
Can you help my question with product A16546, anti-ZNF682/Zinc Finger Protein 682 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2018-01-25
Answer
We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A16546 anti-ZNF682/Zinc Finger Protein 682 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2018-01-25