Product Info Summary
SKU: | A09485 |
---|---|
Size: | 0.1 mg |
Reactive Species: | Human, Mouse |
Host: | Rabbit |
Application: | ELISA, IF, IHC-P, WB |
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Product info
Product Name
Anti-Zinc finger protein DPF3 DPF3 Antibody
SKU/Catalog Number
A09485
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-Zinc finger protein DPF3 DPF3 Antibody (Catalog # A09485). Tested in ELISA, WB, IHC-P, IF applications. This antibody reacts with Human, Mouse.
Storage & Handling
DPF3 antibody can be stored at 4°C for three months and -20°C, stable for up to one year.
Cite This Product
Anti-Zinc finger protein DPF3 DPF3 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A09485)
Host
Rabbit
Contents
DPF3 antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
DPF3 antibody was raised against an 18 amino acid peptide near the carboxy terminus of human DPF3. The immunogen is located within amino acids 220 - 270 of DPF3.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
DPF3 antibody is human and mouse reactive. Multiple isoforms of DPF3 are known to exist.
Reactive Species
A09485 is reactive to DPF3 in Human, Mouse
Reconstitution
Observed Molecular Weight
68 kDa
Calculated molecular weight
43084 MW
Background of DPF3
The DPF3 protein, also known as Cerd4, is a member of the d4 gene family of transcription modulators that also includes DPF1/Neud4 and DPF2/Requiem (1). DPF3 has been shown to be a epigenetic key factor for heart and muscle development and can bind to methylated and acetylated lysine residues of histone 3 and 4, suggesting that DPF3 may play a role in recruiting chromatin remodeling complexes to acetylated histones (2). Two isoforms of DPF3, DPF3a and DPF3b, are required as transcriptional co-activators in SWI/SNF complex-dependent activation of the NF-kappaB RelA/p50 heterodimer (3).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A09485 is guaranteed for ELISA, IF, IHC-P, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
DPF3 antibody can be used for detection of DPF3 by Western blot at 1 - 2 μg/ml. Antibody can also be used for Immunohistochemistry at 5 μg/mL. For Immunoflorescence start at 20 μg/mL.
Antibody validated: Western Blot in mouse samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Western blot analysis of DPF3 in mouse brain tissue lysate with DPF3 antibody at (A) 1 and (B) 2 μg/ml.
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Immunohistochemistry of DPF3 in human brain tissue with DPF3 antibody at 5 μg/mL.
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Immunofluorescence of DPF3 in human brain tissue with DPF3 antibody at 20 μg/mL.
Protein Target Info & Infographic
Gene/Protein Information For DPF3 (Source: Uniprot.org, NCBI)
Gene Name
DPF3
Full Name
Zinc finger protein DPF3
Weight
43084 MW
Superfamily
requiem/DPF family
Alternative Names
CERD4, BAF45C, CERD4, Zinc finger protein DPF3, BRG1-associated factor 45C DPF3 BAF45C, CERD4 double PHD fingers 3 zinc finger protein DPF3|BRG1-associated factor 45C|D4, zinc and double PHD fingers, family 3|zinc finger protein cer-d4
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on DPF3, check out the DPF3 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for DPF3: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Zinc finger protein DPF3 DPF3 Antibody (A09485)
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