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Product Info Summary
| SKU: | A04537 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | ELISA, IF, ICC, WB |
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Product info
Product Name
Anti-ZIMP10 ZMIZ1 Antibody
View all ZMIZ1/Zimp10 Antibodies
SKU/Catalog Number
A04537
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-ZIMP10 ZMIZ1 Antibody (Catalog # A04537). Tested in ELISA, WB, ICC, IF applications. This antibody reacts with Human.
Storage & Handling
ZIMP10 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Cite This Product
Anti-ZIMP10 ZMIZ1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A04537)
Host
Rabbit
Contents
ZIMP10 Antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
ZIMP10 antibody was raised against a 15 amino acid synthetic peptide near the amino terminus of human ZIMP10. The immunogen is located within amino acids 90 - 140 of ZIMP10.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
At least three isoforms are known to exist; this antibody will recognize all three. ZIMP10 antibody is predicted to not cross-react with other PIAS protein family members.
Reactive Species
A04537 is reactive to ZMIZ1 in Human
Reconstitution
Observed Molecular Weight
68 kDa
Calculated molecular weight
115483 MW
Background of ZMIZ1/Zimp10
ZIMP10, also known as ZMIZ1, is a novel PIAS (protein inhibitor of activated signal transducer and activator of transcription)-like protein initially identified as a transcriptional co-activator of the androgen receptor (AR). ZIMP10 and the related protein ZIMP7 interact with PIAS3 and enhances AR-mediated transcription. Later experiments showed that ZIMP10 is also a co-activator of the p53 tumor suppressor. Mice deficient in ZIMP10 result in embryonic lethality by E10.5; these embryos reveal severe defects in the reorganization of the yolk sac vascular plexus, indicating that ZIMP10 plays an important role in proper vascular development.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A04537 is guaranteed for ELISA, IF, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
ZIMP10 antibody can be used for detection of ZIMP10 by Western blot at 0.5 μg/mL. Antibody can also be used for immunocytochemistry starting at 10 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Western blot analysis of ZIMP10 in K562 cell lysate with ZIMP10 antibody at 0.5 μg/mL in (A) the absence and (B) the presence of blocking peptide
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Immunocytochemistry of ZIMP10 in K562 cells with ZIMP10 antibody at 10 μg/mL.
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Immunofluorescence of ZIMP10 in K562 cells with ZIMP10 antibody at 20 μg/mL.
Protein Target Info & Infographic
Gene/Protein Information For ZMIZ1 (Source: Uniprot.org, NCBI)
Gene Name
ZMIZ1
Full Name
Zinc finger MIZ domain-containing protein 1
Weight
115483 MW
Alternative Names
MIZ, RAI17, ZIMP10, hZIMP10, TRAFIP10, KIAA1224, Zinc finger MIZ domain-containing protein 1, PIAS-like protein Zimp10 ZMIZ1 MIZ, NEDDFSA, RAI17, TRAFIP10, ZIMP10 zinc finger MIZ-type containing 1 zinc finger MIZ domain-containing protein 1|retinoic acid induced 17|zinc finger-containing, Miz1, PIAS-like protein on chromosome 10
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on ZMIZ1, check out the ZMIZ1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for ZMIZ1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-ZIMP10 ZMIZ1 Antibody (A04537)
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No publications found for A04537
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