Product Info Summary
SKU: | A03936-2 |
---|---|
Size: | 100 μg/vial |
Reactive Species: | Human, Mouse, Rat |
Host: | Rabbit |
Application: | ELISA, Flow Cytometry, IF, ICC, WB |
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Product info
Product Name
Anti-ZIC2 Antibody Picoband®
SKU/Catalog Number
A03936-2
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-ZIC2 Antibody Picoband® catalog # A03936-2. Tested in ELISA, Flow Cytometry, IF, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-ZIC2 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A03936-2)
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Standard Protein
E.coli-derived human ZIC2 recombinant protein (Position: A31-Y214).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A03936-2 is reactive to ZIC2 in Human, Mouse, Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
51 kDa
Background of ZIC2
Zinc finger protein ZIC2 is a protein that in humans is encoded by the ZIC2 gene. This gene encodes a member of the ZIC family of C2H2-type zinc finger proteins. This protein functions as a transcriptional repressor and may regulate tissue specific expression of dopamine receptor D1. Expansion of an alanine repeat in the C-terminus of the encoded protein and other mutations in this gene cause holoprosencephaly type 5. Holoprosencephaly is the most common structural anomaly of the human brain. A polyhistidine tract polymorphism in this gene may be associated with increased risk of neural tube defects. This gene is closely linked to a gene encoding zinc finger protein of the cerebellum 5, a related family member on chromosome 13.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A03936-2 is guaranteed for ELISA, Flow Cytometry, IF, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5μg/ml, Human, Mouse, Rat
Immunocytochemistry/Immunofluorescence, 5μg/ml, Human
Flow Cytometry (Fixed), 1-3μg/1x106 cells, Human
Direct ELISA, 0.1-0.5μg/ml, Human
Positive Control
WB: human HEK293 whole cell, human THP-1 whole cell, human A549 whole cell, human HepG2 whole cell, rat brain tissue, rat C6 whole cell, rat PC-12 whole cell, mouse brain tissue
ICC/IF: A549 cell
FCM: MCF-7 cell
Validation Images & Assay Conditions
![a03936 2 zic2 primary antibodies wb testing 1 a03936 2 zic2 primary antibodies wb testing 1](https://www.bosterbio.com/media/catalog/product/a/0/a03936-2-zic2-primary-antibodies-wb-testing-1.jpg)
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Figure 1. Western blot analysis of ZIC2 using anti-ZIC2 antibody (A03936-2).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30ug of sample under reducing conditions.
Lane 1: human HEK293 whole cell lysates,
Lane 2: human THP-1 whole cell lysates,
Lane 3: human A549 whole cell lysates,
Lane 4: human HepG2 whole cell lysates,
Lane 5: rat brain tissue lysates,
Lane 6: rat C6 whole cell lysates,
Lane 7: rat PC-12 whole cell lysates,
Lane 8: mouse brain tissue lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ZIC2 antigen affinity purified polyclonal antibody (Catalog # A03936-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ZIC2 at approximately 51KD. The expected band size for ZIC2 is at 51KD.
![a03936 2 zic2 primary antibodies if testing 2 a03936 2 zic2 primary antibodies if testing 2](https://www.bosterbio.com/media/catalog/product/a/0/a03936-2-zic2-primary-antibodies-if-testing-2.jpg)
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Figure 2. IF analysis of ZIC2 using anti-ZIC2 antibody (A03936-2).
ZIC2 was detected in immunocytochemical section of A549 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5μg/mL rabbit anti-ZIC2 Antibody (A03936-2) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
![a03936 2 zic2 primary antibodies fcm testing 3 a03936 2 zic2 primary antibodies fcm testing 3](https://www.bosterbio.com/media/catalog/product/a/0/a03936-2-zic2-primary-antibodies-fcm-testing-3.jpg)
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Figure 3. Flow Cytometry analysis of MCF-7 cells using anti-ZIC2 antibody (A03936-2).
Overlay histogram showing MCF-7 cells stained with A03936-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-ZIC2 Antibody (A03936-2, 1μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Protein Target Info & Infographic
Gene/Protein Information For ZIC2 (Source: Uniprot.org, NCBI)
Gene Name
ZIC2
Full Name
Zinc finger protein ZIC 2
Weight
Superfamily
GLI C2H2-type zinc-finger protein family
Alternative Names
HPE5Zic family member 2 (odd-paired Drosophila homolog); Zic family member 2 (odd-paired homolog, Drosophila); Zinc finger protein of the cerebellum 2; zinc finger protein ZIC 2 ZIC2 HPE5 Zic family member 2 zinc finger protein ZIC 2|Zic family member 2 (odd-paired homolog, Drosophila)|Zinc finger protein of the cerebellum 2
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on ZIC2, check out the ZIC2 Infographic
![ZIC2 infographic](/media/images/gene-infographic-example.jpg)
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for ZIC2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-ZIC2 Antibody Picoband® (A03936-2)
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