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Product Info Summary
| SKU: | A05947 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC-P, WB |
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Product info
Product Name
Anti-ZFX Antibody
SKU/Catalog Number
A05947
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-ZFX Antibody (Catalog # A05947). Tested in ELISA, WB, IHC-P, IF applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
ZFX antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Cite This Product
Anti-ZFX Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A05947)
Host
Rabbit
Contents
ZFX Antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
ZFX antibody was raised against a 19 amino acid synthetic peptide near the carboxy terminus of human ZFX. The immunogen is located within the last 50 amino acids of ZFX.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
ZFX antibody is human, mouse and rat reactive. Multiple isoforms of ZFX are known to exist. ZFX antibody will detect isoform 1 and 2 of ZFX.
Reactive Species
A05947 is reactive to ZFX in Human, Mouse, Rat
Applications
A05947 is guaranteed for ELISA, IF, IHC-P, WB Boster Guarantee
Observed Molecular Weight
68 kDa
Calculated molecular weight
90522 MW
Background of ZFX
The X-linked zinc finger protein ZFX is a member of the Kruppel C2H2-type zinc-finger protein family. The full-length protein contains an acidic transcriptional activation domain (AD), a nuclear localization sequence (NLS) and a DNA binding domain (DBD) consisting of 13 C2H2-type zinc fingers. Studies in mouse embryonic and adult hematopoietic stem cells showed that this gene was required as a transcriptional regulator for self-renewal of both stem cell types, but it was dispensable for growth and differentiation of their progeny.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
ZFX antibody can be used for detection of ZFX by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 5 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Western blot analysis of ZFX in Daudi cell lysate with ZFX antibody at 1 μg/mL.
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Immunohistochemistry of ZFX in human small intestine tissue with ZFX antibody at 5 μg/mL.
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Immunofluorescence of ZFX in human small intestine tissue with ZFX antibody at 20 μg/mL.
Protein Target Info & Infographic
Gene/Protein Information For ZFX (Source: Uniprot.org, NCBI)
Gene Name
ZFX
Full Name
Zinc finger X-chromosomal protein
Weight
90522 MW
Superfamily
krueppel C2H2-type zinc-finger protein family
Alternative Names
zinc finger protein, X-linked ZFX ZNF926 zinc finger protein X-linked zinc finger X-chromosomal protein|X-linked zinc finger protein
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on ZFX, check out the ZFX Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for ZFX: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-ZFX Antibody (A05947)
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