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Product Info Summary
| SKU: | A02668 |
|---|---|
| Size: | 100 µl |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | IHC |
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Product info
Product Name
Anti-ZFHX3/Atbf1 Antibody
SKU/Catalog Number
A02668
Size
100 µl
Form
Liquid
Description
Boster Bio Anti-ZFHX3/Atbf1 Antibody (Catalog # A02668). Tested in IHC applications. This antibody reacts with Human, Mouse.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-ZFHX3/Atbf1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A02668)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthesized peptide derived from internal of human ZFHX3.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A02668 is reactive to ZFHX3 in Human, Mouse
Reconstitution
Calculated molecular weight
404419 MW
Background of ZFHX3
Transcriptional repressor. It inhibits the enhancer element of the AFP gene by binding to its AT-rich core sequence. Regulator of myoblasts differentiation through the binding to the AT-rich sequence of MYF6 promoter and promoter repression. Down-regulates the MUC5AC promoter in gastric cancer.
Miura Y., J. Biol. Chem. 270:26840-26848(1995).
Morinaga T., Mol. Cell. Biol. 11:6041-6049(1991).
Martin J., Nature 432:988-994(2004).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A02668 is guaranteed for IHC Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunohistochemistry: 1:50~1:100
Validation Images & Assay Conditions
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Immunohistochemistry analysis of paraffin-embedded human brain tissue, using ZFHX3 antibody A02668.
Protein Target Info & Infographic
Gene/Protein Information For ZFHX3 (Source: Uniprot.org, NCBI)
Gene Name
ZFHX3
Full Name
Zinc finger homeobox protein 3
Weight
404419 MW
Alternative Names
Zinc finger homeobox protein 3;AT motif-binding factor 1;AT-binding transcription factor 1;Alpha-fetoprotein enhancer-binding protein;Zinc finger homeodomain protein 3;ZFH-3;ZFHX3;ATBF1; ZFHX3 ATBF1, ATBT, C16orf47, ZFH-3, ZNF927 zinc finger homeobox 3 zinc finger homeobox protein 3|AT motif-binding factor 1|AT-binding transcription factor 1|alpha-fetoprotein enhancer binding protein|zinc finger homeodomain protein 3
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on ZFHX3, check out the ZFHX3 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for ZFHX3: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-ZFHX3/Atbf1 Antibody (A02668)
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Customer Q&As
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6 Customer Q&As for Anti-ZFHX3/Atbf1 Antibody
Question
I was wanting to use your anti-ZFHX3/Atbf1 antibody for IHC for human hepatoma on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human hepatoma identification?
Verified Customer
Verified customer
Asked: 2020-01-27
Answer
You can see on the product datasheet, A02668 anti-ZFHX3/Atbf1 antibody has been tested for IHC on human, mouse tissues. We have an innovator award program that if you test this antibody and show it works in human hepatoma in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2020-01-27
Question
Do you have a BSA free version of anti-ZFHX3/Atbf1 antibody A02668 available?
Verified Customer
Verified customer
Asked: 2019-07-09
Answer
I appreciate your recent telephone inquiry. I can confirm that some lots of this anti-ZFHX3/Atbf1 antibody A02668 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-07-09
Question
We are currently using anti-ZFHX3/Atbf1 antibody A02668 for human tissue, and we are satisfied with the IHC results. The species of reactivity given in the datasheet says human, mouse. Is it true that the antibody can work on horse tissues as well?
Verified Customer
Verified customer
Asked: 2019-06-28
Answer
The anti-ZFHX3/Atbf1 antibody (A02668) has not been validated for cross reactivity specifically with horse tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in horse you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-06-28
Question
I have a question about product A02668, anti-ZFHX3/Atbf1 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2018-01-15
Answer
We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A02668 anti-ZFHX3/Atbf1 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2018-01-15
Question
I would like to test anti-ZFHX3/Atbf1 antibody A02668 on human hepatoma for research purposes, then I may be interested in using anti-ZFHX3/Atbf1 antibody A02668 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2017-11-03
Answer
The products we sell, including anti-ZFHX3/Atbf1 antibody A02668, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2017-11-03
Question
Will anti-ZFHX3/Atbf1 antibody A02668 work for IHC with hepatoma?
Verified Customer
Verified customer
Asked: 2017-10-11
Answer
According to the expression profile of hepatoma, ZFHX3 is highly expressed in hepatoma. So, it is likely that anti-ZFHX3/Atbf1 antibody A02668 will work for IHC with hepatoma.
Boster Scientific Support
Answered: 2017-10-11
