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Product Info Summary
| SKU: | A00694-2 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, Flow Cytometry, IHC, WB |
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Product info
Product Name
Anti-XPD/ERCC2 Antibody Picoband®
SKU/Catalog Number
A00694-2
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-XPD/ERCC2 Antibody Picoband® catalog # A00694-2. Tested in ELISA, Flow Cytometry, IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-XPD/ERCC2 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00694-2)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human XPD/ERCC2 recombinant protein (Position: E79-A295).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A00694-2 is reactive to ERCC2 in Human, Mouse, Rat
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Observed Molecular Weight
87 kDa
Calculated molecular weight
86.909kDa
Background of XPD
ERCC2, or XPD is a protein involved in transcription-coupled nucleotide excision repair. The nucleotide excision repair pathway is a mechanism to repair damage to DNA. The protein encoded by this gene is involved in transcription-coupled nucleotide excision repair and is an integral member of the basal transcription factor BTF2/TFIIH complex. The gene product has ATP-dependent DNA helicase activity and belongs to the RAD3/XPD subfamily of helicases. Defects in this gene can result in three different disorders, the cancer-prone syndrome xeroderma pigmentosum complementation group D, trichothiodystrophy, and Cockayne syndrome. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A00694-2 is guaranteed for ELISA, Flow Cytometry, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5 μg/ml, Human
Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/ml, Human, Mouse, Rat
Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human
ELISA, 0.1-0.5 μg/ml, -
Positive Control
WB: human K562 whole cell, human SiHa whole cell, human HepG2 whole cell
IHC: human bladder epithelial carcinom tissue, mouse testis tissue, rat testis tissue
FCM: THP-1 cell
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of XPD/ERCC2 using anti-XPD/ERCC2 antibody (A00694-2).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human K562 whole cell lysates,
Lane 2: human SiHa whole cell lysates,
Lane 3: human HepG2 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-XPD/ERCC2 antigen affinity purified polyclonal antibody (Catalog # A00694-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for XPD/ERCC2 at approximately 87 kDa. The expected band size for XPD/ERCC2 is at 80 kDa.
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Figure 2. IHC analysis of XPD/ERCC2 using anti-XPD/ERCC2 antibody (A00694-2).
XPD/ERCC2 was detected in a paraffin-embedded section of human bladder epithelial carcinom tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-XPD/ERCC2 Antibody (A00694-2) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
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Figure 3. IHC analysis of XPD/ERCC2 using anti-XPD/ERCC2 antibody (A00694-2).
XPD/ERCC2 was detected in a paraffin-embedded section of mouse testis tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-XPD/ERCC2 Antibody (A00694-2) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
Click image to see more details
Figure 4. IHC analysis of XPD/ERCC2 using anti-XPD/ERCC2 antibody (A00694-2).
XPD/ERCC2 was detected in a paraffin-embedded section of rat testis tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-XPD/ERCC2 Antibody (A00694-2) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
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Figure 5. Flow Cytometry analysis of THP-1 cells using anti-XPD/ERCC2 antibody (A00694-2).
Overlay histogram showing THP-1 cells stained with A00694-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-XPD/ERCC2 Antibody (A00694-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Protein Target Info & Infographic
Gene/Protein Information For ERCC2 (Source: Uniprot.org, NCBI)
Gene Name
ERCC2
Full Name
General transcription and DNA repair factor IIH helicase subunit XPD
Weight
86.909kDa
Superfamily
helicase family
Alternative Names
Early growth response protein 1 ERCC2 COFS2, EM9, TFIIH, TTD, TTD1, XPD ERCC excision repair 2, TFIIH core complex helicase subunit general transcription and DNA repair factor IIH helicase subunit XPD|BTF2 p80|CXPD|DNA excision repair protein ERCC-2|DNA repair protein complementing XP-D cells|TFIIH 80 kDa subunit|TFIIH basal transcription factor complex 80 kDa subunit|TFIIH basal transcription factor complex helicase XPB subunit|TFIIH basal transcription factor complex helicase XPD subunit|TFIIH basal transcription factor complex helicase subunit|TFIIH p80|TFIIH subunit XPD|basic transcription factor 2 80 kDa subunit|excision repair cross-complementation group 2|excision repair cross-complementing rodent repair deficiency, complementation group 2|xeroderma pigmentosum complementary group D|xeroderma pigmentosum group D-complementing protein
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on ERCC2, check out the ERCC2 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for ERCC2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-XPD/ERCC2 Antibody Picoband® (A00694-2)
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