Anti-Phospho-XIRP1 (Ser295) Antibody

Product Name

Anti-Phospho-XIRP1 (Ser295) Antibody

View all Xirp1 Antibodies

SKU/Catalog Number

P07785

Size

100 µl

Form

Liquid

Description

Boster Bio Anti-Phospho-XIRP1 (Ser295) Antibody (Catalog # P07785). Tested in WB applications. This antibody reacts with Mouse.

Storage & Handling

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C. After date of receipt, stable for at least 1 year at -20°C.

Cite This Product

Anti-Phospho-XIRP1 (Ser295) Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # P07785)

Host

Rabbit

Contents

10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol.

Clonality

Polyclonal

Isotype

IgG

Immunogen

Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser295 of mouse XIRP, conjugated to keyhole limpet hemocyanin (KLH). Immunogen species is Mouse.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross reactivity with other proteins.

Reactive Species

P07785 is reactive to XIRP1 in Mouse

Reconstitution

Restore with deionized water (or equivalent) for reconstitution volume of 100 µL

Observed Molecular Weight

52 kDa

Calculated molecular weight

198561 MW

Background of Xirp1

XIRP1 is a member of the Xin family of proteins, containing 16 Xin repeats. The intercalated disk protein Xin protects actin filaments from depolymerization, and is able to bundle actin filaments to interact with β-catenin, which is thought to stabilize actin-based cytoskeletons (Choi et al., 2007). The evolutionary emergence of the Xin paralogs may have played a key role in the development of heart chambers with complete endothelial and myocardial layers (Grosskurth et al., 2008). Loss of mXina (mouse Xin homolog) results in cardiac hypertrophy and cardiomyopathy (Gustafson-Wagner et al., 2007). Phosphorylation of Ser-295 plays a key role in XIRP1 signaling, and therefore in cardiac hypertrophy, as it has been shown that acute pressure-overloaded hearts rapidly increased phosphorylation at the Ser-295 residue of XIRP1. (Chang et al., 2013).

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

P07785 is guaranteed for WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

WB: 1:1000

Validation Images & Assay Conditions

Gene/Protein Information For XIRP1 (Source: Uniprot.org, NCBI)

Alternative Names

AI415219 antibody, Cardiomyopathy-associated protein 1 antibody, CMYA1 antibody, DKFZp451D042 antibody, DKFZp779C1255 antibody, DKFZp779C1947 antibody, MGC144145 antibody, MGC144146 antibody, Xin actin-binding repeat-containing protein 1 antibody, XIN antibody, Xirp1 antibody, XIRP1_HUMAN antibody Xirp1|AI415219, Cmya, Cmya1, X|xin actin-binding repeat containing 1|xin actin-binding repeat-containing protein 1|Xin alpha|cardiomyopathy associated 1|cardiomyopathy-associated protein 1|mXin alpha

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

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