Product Info Summary
SKU: | A06649 |
---|---|
Size: | 100 µl |
Reactive Species: | Human, Mouse |
Host: | Rabbit |
Application: | WB |
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Product info
Product Name
Anti-VTI1A/V Snare Vti1A Antibody
SKU/Catalog Number
A06649
Size
100 µl
Form
Liquid
Description
Boster Bio Anti-VTI1A/V Snare Vti1A Antibody (Catalog # A06649). Tested in WB applications. This antibody reacts with Human, Mouse.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-VTI1A/V Snare Vti1A Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A06649)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthesized peptide derived from internal of human VTI1A.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
A06649 is reactive to VTI1A in Human, Mouse
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 100 µL
Calculated molecular weight
25218 MW
Background of Vti1a
V-SNARE that mediates vesicle transport pathways through interactions with t-SNAREs on the target membrane. These interactions are proposed to mediate aspects of the specificity of vesicle trafficking and to promote fusion of the lipid bilayers. Involved in vesicular transport from the late endosomes to the trans-Golgi network. Along with VAMP7, involved in an non-conventional RAB1-dependent traffic route to the cell surface used by KCNIP1 and KCND2. May be involved in increased cytokine secretion associated with cellular senescence. UniProtKB O89116
Ota T., Nat. Genet. 36:40-45(2004).
Deloukas P., Nature 429:375-381(2004).
Mural R.J., Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A06649 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blotting: 1:500~1:3000
Validation Images & Assay Conditions
Click image to see more details
Western blot analysis of extracts from 3T3 cells, using VTI1A antibody A06649.
Protein Target Info & Infographic
Gene/Protein Information For VTI1A (Source: Uniprot.org, NCBI)
Gene Name
VTI1A
Full Name
Vesicle transport through interaction with t-SNAREs homolog 1A
Weight
25218 MW
Superfamily
VTI1 family
Alternative Names
Vesicle transport through interaction with t-SNAREs homolog 1A;Vesicle transport v-SNARE protein Vti1-like 2;Vti1-rp2;VTI1A; Vti1a|1110014F16Rik, 1110018K19Rik, 4921537J05Rik, MVti1, MVti1a, Vti1, Vti1-rp2|vesicle transport through interaction with t-SNAREs 1A|vesicle transport through interaction with t-SNAREs homolog 1A|vesicle transport through interaction with t-SNAREs 1 homolog|vesicle transport v-SNARE protein Vti1-like 2
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on VTI1A, check out the VTI1A Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for VTI1A: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-VTI1A/V Snare Vti1A Antibody (A06649)
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No publications found for A06649
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Customer Q&As
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6 Customer Q&As for Anti-VTI1A/V Snare Vti1A Antibody
Question
We are currently using anti-VTI1A/V Snare Vti1A antibody A06649 for human tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human, mouse. Is it true that the antibody can work on monkey tissues as well?
Verified Customer
Verified customer
Asked: 2020-01-21
Answer
The anti-VTI1A/V Snare Vti1A antibody (A06649) has not been validated for cross reactivity specifically with monkey tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in monkey you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-01-21
Question
Will anti-VTI1A/V Snare Vti1A antibody A06649 work on bovine WB with corpus callosum?
Verified Customer
Verified customer
Asked: 2019-12-16
Answer
Our lab technicians have not tested anti-VTI1A/V Snare Vti1A antibody A06649 on bovine. You can run a BLAST between bovine and the immunogen sequence of anti-VTI1A/V Snare Vti1A antibody A06649 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated bovine samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in bovine corpus callosum in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-12-16
Question
Is there a BSA free version of anti-VTI1A/V Snare Vti1A antibody A06649 available?
Verified Customer
Verified customer
Asked: 2019-09-24
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-VTI1A/V Snare Vti1A antibody A06649 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-09-24
Question
I see that the anti-VTI1A/V Snare Vti1A antibody A06649 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-05-28
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2019-05-28
Question
Please see the WB image, lot number and protocol we used for lung using anti-VTI1A/V Snare Vti1A antibody A06649. Please let me know if you require anything else.
P. Anderson
Verified customer
Asked: 2019-03-05
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-03-05
Question
Is this A06649 anti-VTI1A/V Snare Vti1A antibody reactive to the isotypes of VTI1A?
Verified Customer
Verified customer
Asked: 2019-01-07
Answer
The immunogen of A06649 anti-VTI1A/V Snare Vti1A antibody is Synthesized peptide derived from internal of human VTI1A. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-01-07