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Product Info Summary
| SKU: | A07809-1 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, Flow Cytometry, WB |
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Product info
Product Name
Anti-VPS36 Antibody Picoband®
SKU/Catalog Number
A07809-1
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-VPS36 Antibody Picoband® catalog # A07809-1. Tested in ELISA, Flow Cytometry, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-VPS36 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A07809-1)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human VPS36 recombinant protein (Position: M1-S386).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A07809-1 is reactive to VPS36 in Human, Mouse, Rat
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Observed Molecular Weight
44 kDa
Calculated molecular weight
49229 MW
Background of VPS36
Vacuolar protein-sorting-associated protein 36 is a protein that in humans is encoded by the VPS36 gene. This gene encodes a protein that is a subunit of the endosomal sorting complex required for transport II (ESCRT-II). This protein complex functions in sorting of ubiquitinated membrane proteins during endocytosis. A similar protein complex in rat is associated with RNA polymerase elongation factor II.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A07809-1 is guaranteed for ELISA, Flow Cytometry, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5 μg/ml/ml, Human, Mouse, Rat
Flow Cytometry (Fixed), 1-3 μg/ml/1x10^6 cells, Human
ELISA, 0.1-0.5 μg/ml/ml, Human
Positive Control
WB: human HL-60 whole cell, human THP-1 whole cell, human MOLT-4 whole cell, human U937 whole cell, rat brain tissue, mouse brain tissue
FCM: PC-3 cell
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of VPS36 using anti-VPS36 antibody (A07809-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human HL-60 whole cell lysates,
Lane 2: human THP-1 whole cell lysates,
Lane 3: human MOLT-4 whole cell lysates,
Lane 4: human U937 whole cell lysates,
Lane 5: rat brain tissue lysates,
Lane 6: mouse brain tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-VPS36 antigen affinity purified polyclonal antibody (Catalog # A07809-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for VPS36 at approximately 44 kDa. The expected band size for VPS36 is at 44 kDa.
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Figure 2. Flow Cytometry analysis of PC-3 cells using anti-VPS36 antibody (A07809-1).
Overlay histogram showing PC-3 cells stained with A07809-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-VPS36 Antibody (A07809-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Protein Target Info & Infographic
Gene/Protein Information For VPS36 (Source: Uniprot.org, NCBI)
Gene Name
VPS36
Full Name
Vacuolar protein-sorting-associated protein 36
Weight
49229 MW
Superfamily
VPS36 family
Alternative Names
Protein SOX-15; Protein SOX-12; Protein SOX-20; SOX15; SOX12; SOX20; SOX26; SOX27 VPS36 C13orf9, CGI-145, EAP45 vacuolar protein sorting 36 homolog vacuolar protein-sorting-associated protein 36|ELL-associated protein of 45 kDa|ESCRT-II complex subunit VPS36
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on VPS36, check out the VPS36 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for VPS36: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-VPS36 Antibody Picoband® (A07809-1)
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