Anti-VPS29 Antibody

Product Name

Anti-VPS29 Antibody

View all VPS29 Antibodies

SKU/Catalog Number

A04097

Size

100ul

Form

Liquid

Description

Boster Bio Anti-VPS29 Antibody catalog # A04097. Tested in WB,ICC/IF applications. This antibody reacts with Human,Mouse,Rat.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-VPS29 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A04097)

Host

Rabbit

Contents

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Clonality

Polyclonal

Isotype

IgG

Immunogen

Anti-Bach2 antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to the n-terminus region of the Bach2 protein.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

A04097 is reactive to VPS29 in Human, Mouse, Rat

Applications

A04097 is guaranteed for IF, ICC, WB Boster Guarantee

Observed Molecular Weight

Calculated molecular weight

20506 MW

Background of VPS29

This antibody is suitable for Cancer and DNA Damage & Repair research. During an immune response to infectious diseases and cancer, B-cells undergo activation in response to antigen, and differentiate either into short-lived plasma cells, whose numbers contract following withdrawal of the antigenic stimulus, or into memory B-cells. The formation of B-cell memory is the major goal of vaccination, since memory B-cells are able to produce antibodies specific to infections and cancer that can last for the lifetime of the host. Recently it has been shown that the formation of long-lived memory B-cells is dependent upon the transcription factor Bach2, since knockout mice lacking the gene fail to generate class-switched IgG antibody responses and all B-cells undergo default plasma cell differentiation upon antigenic stimulation. While the mechanism by which Bach2 controls B-cell memory fate has been elucidated (it binds and represses the promoter of the gene that encodes the plasma cell master regulatory transcription factor Blimp-1), the upstream mechanism by which the function of Bach2 is regulated is unknown. There is evidence suggesting that Bach2 is phosphorylated in B-cells following stimulation and suspect that this modification may allow the B-cell signaling apparatus to control Bach2 activity and therefore memory fate decisions. Anti-Bach2 is ideal for researchers interested in Cancer and DNA Damage & Repair research.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

WB: 1:500-1:2000
ICC/IF: 1:50-1:200

Validation Images & Assay Conditions

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Figure 1. Western blotting validation for Anti-VPS29 Antibody A04097

Western blot analysis of extracts of various cell lines

Gene/Protein Information For VPS29 (Source: Uniprot.org, NCBI)

Alternative Names

DC15; DC7; DKFZp564F0223; EC 3.1.3.3; FLJ20492; hVPS29; PEP11 homolog; PEP11; retromer protein; vacuolar protein sorting 29 (yeast homolog); vacuolar protein sorting 29 (yeast); vacuolar protein sorting 29 homolog (S. cerevisiae); vacuolar protein sorting-associated protein 29; vacuolar sorting protein VPS29/PEP11; Vesicle protein sorting 29; x 007 protein VPS29 DC15, DC7, PEP11 VPS29 retromer complex component vacuolar protein sorting-associated protein 29|PEP11 homolog|epididymis secretory sperm binding protein|hVPS29|retromer protein|vacuolar protein sorting 29 homolog|vacuolar sorting protein VPS29/PEP11|vesicle protein sorting 29|x 007 protein

For more info on VPS29, check out the VPS29 Infographic

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In this infographic, you will see the following information for VPS29: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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