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Product Info Summary
| SKU: | M00235-6 |
|---|---|
| Size: | 100uL |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | IHC, WB |
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Product info
Product Name
Anti-Vimentin Rabbit Monoclonal Antibody, Clone#RM289
SKU/Catalog Number
M00235-6
Size
100uL
Form
Liquid
Description
Boster Bio Anti-Vimentin Rabbit Monoclonal Antibody, Clone#RM289 (Catalog # M00235-6). Tested in IHC, WB applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Vimentin Rabbit Monoclonal Antibody, Clone#RM289 (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00235-6)
Host
Rabbit
Contents
50% Glycerol/PBS with 1% BSA and 0.09% sodium azide
Clonality
Monoclonal
Clone Number
RM289
Isotype
Rabbit IgG
Immunogen
A peptide corresponding to the C-terminus of human Vimentin.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
This antibody reacts to human Vimentin. It may also react to mouse or rat Vimentin, as predicted by immunogen homology.
Reactive Species
M00235-6 is reactive to VIM in Human
Applications
M00235-6 is guaranteed for IHC, WB Boster Guarantee
Observed Molecular Weight
Calculated molecular weight
53652 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunohistochemistry (IHC): 1:100-1:200 dilution
WB: 1:200-1:500 dilution.
Validation Images & Assay Conditions
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Figure 1. Western Blotting result
Western Blot of HeLa lysates using anti-Vimentin rabbit monoclonal antibody (clone RM289) at a 1:400 dilution.
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Figure 2. IHC result
Immunohistochemical staining of formalin fixed and paraffin embedded human kidney tissue sections using anti-Vimentin rabbit monoclonal antibody (clone RM289) at a 1:200 dilution.
Protein Target Info & Infographic
Gene/Protein Information For VIM (Source: Uniprot.org, NCBI)
Gene Name
VIM
Full Name
Vimentin
Weight
53652 MW
Superfamily
intermediate filament family
Alternative Names
FLJ36605; VIM; Vimentin VIM vimentin vimentin|epididymis secretory sperm binding protein
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on VIM, check out the VIM Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for VIM: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Vimentin Rabbit Monoclonal Antibody, Clone#RM289 (M00235-6)
Hello CJ!
M00235-6 has been cited in 25 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Zhang XW,Li QH,Xu ZD,Dou JJ.STAT1-induced regulation of lncRNA ZFPM2-AS1 predicts poor prognosis and contributes to hepatocellular carcinoma progression via the miR-653/GOLM1 axis.Cell Death Dis.2021 Jan 4;12(1):31.doi:10.1038/s41419-020-03300-4.PMID:3341
Species: Human,Mouse
M00235-6 usage in article: APP:WB, SAMPLE:HCC CELL, DILUTION:1:800
Jiang SB,Lu YS,Liu T,Li LM,Wang HX,Wu Y,Gao XH,Chen HD. UVA influenced the SIRT1-miR-27a-5p-SMAD2-MMP1/COL1/BCL2 axis in human skin primary fibroblasts.J Cell Mol Med.2020 Sep;24(17):10027-10041.doi: 10.1111/jcmm.15610.Epub 2020 Aug 13.PMID:32790210;PMCID
Species: Human
M00235-6 usage in article: APP:IF, SAMPLE:SKIN PRIMARY FIBROBLASTS, DILUTION:1:100
The Role of the RhoA/ROCK Signaling Pathway in Mechanical Strain-Induced Scleral Myofibroblast Differentiation
Tensile strength suppresses the osteogenesis of periodontal ligament cells in inflammatory microenvironments
Autophagy Is Indispensable for the Self-Renewal and Quiescence of Ovarian Cancer Spheroid Cells with Stem Cell-Like Properties
Expression of E-cadherin and vimentin in oral squamous cell carcinoma
%u03B22-microglobulin induces epithelial-mesenchymal transition in human renal proximal tubule epithelial cells in vitro
Distinct Roles For ROCK1 and ROCK2 in the Regulation of Oxldl-Mediated Endothelial Dysfunction
MTDH promotes glioma invasion through regulating miR-130b-ceRNAs
Micro-/nano-topography of selective laser melting titanium enhances adhesion and proliferation and regulates adhesion-related gene expressions of human %u2026
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Customer Reviews
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Customer Q&As
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16 Customer Q&As for Anti-Vimentin Rabbit Monoclonal Antibody, Clone#RM289
Question
We are currently using anti-Vimentin Rabbit Monoclonal antibody, Clone#RM289 M00235-6 for human tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human. Is it true that the antibody can work on pig tissues as well?
Verified Customer
Verified customer
Asked: 2020-04-29
Answer
The anti-Vimentin Rabbit Monoclonal antibody, Clone#RM289 (M00235-6) has not been validated for cross reactivity specifically with pig tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in pig you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-04-29
Question
I have a question about product M00235-6, anti-Vimentin Rabbit Monoclonal antibody, Clone#RM289. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2020-01-23
Answer
It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M00235-6 anti-Vimentin Rabbit Monoclonal antibody, Clone#RM289, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2020-01-23
Question
Does anti-Vimentin Rabbit Monoclonal antibody, Clone#RM289 M00235-6 work for IHC with brain?
Verified Customer
Verified customer
Asked: 2019-12-04
Answer
According to the expression profile of brain, VIM is highly expressed in brain. So, it is likely that anti-Vimentin Rabbit Monoclonal antibody, Clone#RM289 M00235-6 will work for IHC with brain.
Boster Scientific Support
Answered: 2019-12-04
Question
I see that the anti-Vimentin Rabbit Monoclonal antibody, Clone#RM289 M00235-6 works with IHC, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-11-07
Answer
You can find protocols for IHC on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2019-11-07
Question
We have been able to see staining in human dorsal root ganglion. What should we do? Is anti-Vimentin Rabbit Monoclonal antibody, Clone#RM289 supposed to stain dorsal root ganglion positively?
Verified Customer
Verified customer
Asked: 2019-08-15
Answer
From what I have seen in literature dorsal root ganglion does express VIM. From what I have seen in Uniprot.org, VIM is expressed in dorsal root ganglion, lymphoma, testis, embryo, placenta stomach, adipose tissue coronary artery, cervix, placenta testis, mammary carcinoma, t-cell, hepatoma, brain, cajal-retzius cell fetal brain cortex, fibroblast, osteosarcoma, lymphoblast, cervix carcinoma, leukemic t-cell, cervix carcinoma erythroleukemia, liver, among other tissues. Regarding which tissues have VIM expression, here are a few articles citing expression in various tissues:
Cervix carcinoma, Pubmed ID: 16964243, 17081983, 17924679, 18220336, 18669648, 18691976, 20068231
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Embryo, Placenta, and Stomach, Pubmed ID: 14702039
Fibroblast, Pubmed ID: 3371665
Leukemic T-cell, Pubmed ID: 19690332
Liver, Pubmed ID: 24275569
Lymphoblast, Pubmed ID: 14654843
Lymphoma, Pubmed ID: 14996095
Mammary carcinoma, Pubmed ID: 9150946
Osteosarcoma, Pubmed ID: 2323579
Boster Scientific Support
Answered: 2019-08-15
Question
We are interested in to test anti-Vimentin Rabbit Monoclonal antibody, Clone#RM289 M00235-6 on human brain for research purposes, then I may be interested in using anti-Vimentin Rabbit Monoclonal antibody, Clone#RM289 M00235-6 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2019-08-13
Answer
The products we sell, including anti-Vimentin Rabbit Monoclonal antibody, Clone#RM289 M00235-6, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-08-13
Question
I was wanting to use your anti-Vimentin Rabbit Monoclonal antibody, Clone#RM289 for IHC for human brain on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human brain identification?
Verified Customer
Verified customer
Asked: 2019-07-03
Answer
You can see on the product datasheet, M00235-6 anti-Vimentin Rabbit Monoclonal antibody, Clone#RM289 has been validated for IHC, WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human brain in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-07-03
Question
We ordered your anti-Vimentin Rabbit Monoclonal antibody, Clone
Verified Customer
Verified customer
Asked: 2019-06-13
Answer
I took a look at the website and datasheets of our anti-Vimentin Rabbit Monoclonal antibody, Clone
Boster Scientific Support
Answered: 2019-06-13
Question
Is this M00235-6 anti-Vimentin Rabbit Monoclonal antibody, Clone#RM289 reactive to the isotypes of VIM?
Verified Customer
Verified customer
Asked: 2019-05-31
Answer
The immunogen of M00235-6 anti-Vimentin Rabbit Monoclonal antibody, Clone#RM289 is A peptide corresponding to the C-terminus of human Vimentin. . Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-05-31
Question
Here is the WB image, lot number and protocol we used for brain using anti-Vimentin Rabbit Monoclonal antibody, Clone#RM289 M00235-6. Please let me know if you require anything else.
N. Li
Verified customer
Asked: 2018-11-13
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2018-11-13
Question
Is a blocking peptide available for product anti-Vimentin Rabbit Monoclonal antibody, Clone#RM289 (M00235-6)?
Verified Customer
Verified customer
Asked: 2018-10-08
Answer
We do provide the blocking peptide for product anti-Vimentin Rabbit Monoclonal antibody, Clone#RM289 (M00235-6). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2018-10-08
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for brain using anti-Vimentin Rabbit Monoclonal antibody, Clone#RM289 M00235-6. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2018-04-09
Answer
Thank you for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2018-04-09
Question
Do you have a BSA free version of anti-Vimentin Rabbit Monoclonal antibody, Clone#RM289 M00235-6 available?
S. Jones
Verified customer
Asked: 2017-11-22
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-Vimentin Rabbit Monoclonal antibody, Clone#RM289 M00235-6 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2017-11-22
Question
Will M00235-6 anti-Vimentin Rabbit Monoclonal antibody, Clone#RM289 work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2017-07-11
Answer
It shows on the product datasheet, M00235-6 anti-Vimentin Rabbit Monoclonal antibody, Clone#RM289 as been validated on IHC. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2017-07-11
Question
I would like using your anti-Vimentin Rabbit Monoclonal antibody, Clone#RM289 for multiple types (ctrct30) studies. Has this antibody been tested with western blotting on human kidney tissue? We would like to see some validation images before ordering.
A. Krishna
Verified customer
Asked: 2014-11-26
Answer
Thanks for your inquiry. This M00235-6 anti-Vimentin Rabbit Monoclonal antibody, Clone#RM289 is tested on human kidney tissue. It is guaranteed to work for IHC, WB in human. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2014-11-26
Question
My colleagues were content with the WB result of your anti-Vimentin Rabbit Monoclonal antibody, Clone
A. Jha
Verified customer
Asked: 2013-05-24
Answer
According to literature, lymphoma does express VIM. Generally VIM expresses in cytoplasm. Regarding which tissues have VIM expression, here are a few articles citing expression in various tissues:
Cervix carcinoma, Pubmed ID: 16964243, 17081983, 17924679, 18220336, 18669648, 18691976, 20068231
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Embryo, Placenta, and Stomach, Pubmed ID: 14702039
Fibroblast, Pubmed ID: 3371665
Leukemic T-cell, Pubmed ID: 19690332
Liver, Pubmed ID: 24275569
Lymphoblast, Pubmed ID: 14654843
Lymphoma, Pubmed ID: 14996095
Mammary carcinoma, Pubmed ID: 9150946
Osteosarcoma, Pubmed ID: 2323579
Boster Scientific Support
Answered: 2013-05-24
