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Product Info Summary
| SKU: | A00691-1 |
|---|---|
| Size: | 100μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-Proto-oncogene vav VAV1 Antibody
SKU/Catalog Number
A00691-1
Size
100μl
Form
Liquid
Description
Boster Bio Anti-Proto-oncogene vav VAV1 Antibody catalog # A00691-1. Tested in WB applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Proto-oncogene vav VAV1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00691-1)
Host
Rabbit
Contents
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Clonality
Monoclonal
Clone Number
5B5
Isotype
IgG
Immunogen
The antiserum was produced against synthesized peptide derived from human VAV1. AA range:131-180
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
A00691-1 is reactive to VAV1 in Human, Mouse, Rat
Reconstitution
Observed Molecular Weight
39 kDa
Calculated molecular weight
98314 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A00691-1 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB, 1:500-1:2000
Validation Images & Assay Conditions
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Western Blot (WB) analysis of extracts from NIH-3T3 cells, using VAV1 Polyclonal antibody.
Protein Target Info & Infographic
Gene/Protein Information For VAV1 (Source: Uniprot.org, NCBI)
Gene Name
VAV1
Full Name
Proto-oncogene vav
Weight
98314 MW
Alternative Names
Proto-oncogene vav;VAV1;VAV; VAV1 VAV vav guanine nucleotide exchange factor 1 proto-oncogene vav|vav 1 guanine nucleotide exchange factor|vav 1 oncogene
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on VAV1, check out the VAV1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for VAV1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Proto-oncogene vav VAV1 Antibody (A00691-1)
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No publications found for A00691-1
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Customer Q&As
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5 Customer Q&As for Anti-Proto-oncogene vav VAV1 Antibody
Question
Is a blocking peptide available for product anti-VAV1 antibody (A00691-1)?
Verified Customer
Verified customer
Asked: 2019-11-20
Answer
We do provide the blocking peptide for product anti-VAV1 antibody (A00691-1). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2019-11-20
Question
I have a question about product A00691-1, anti-VAV1 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-11-08
Answer
It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A00691-1 anti-VAV1 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-11-08
Question
Does A00691-1 anti-VAV1 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-10-16
Answer
You can see on the product datasheet, A00691-1 anti-VAV1 antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-10-16
Question
We want to test anti-VAV1 antibody A00691-1 on mouse spleen for research purposes, then I may be interested in using anti-VAV1 antibody A00691-1 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
K. Lewis
Verified customer
Asked: 2019-06-11
Answer
The products we sell, including anti-VAV1 antibody A00691-1, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-06-11
Question
I see that the anti-VAV1 antibody A00691-1 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-05-16
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2019-05-16
