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Product Info Summary
| SKU: | A00303 |
|---|---|
| Size: | 100 µl |
| Reactive Species: | Human, Rat |
| Host: | Rabbit |
| Application: | IF, WB |
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Product info
Product Name
Anti-VASP (Ab-157) Antibody
SKU/Catalog Number
A00303
Size
100 µl
Form
Liquid
Description
Boster Bio Anti-VASP (Ab-157) Antibody (Catalog # A00303). Tested in WB, IF applications. This antibody reacts with Human, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-VASP (Ab-157) Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00303)
Host
Rabbit
Contents
Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Peptide sequence around aa.155~159 (R-V-S-N-A) derived from Human VASP.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A00303 is reactive to VASP in Human, Rat
Reconstitution
Observed Molecular Weight
39 kDa
Calculated molecular weight
59257 MW
Background of VASP
Vasodilator-stimulated phosphoprotein (VASP) is a member of the Ena-VASP protein family. Ena-VASP family members contain an EHV1 N-terminal domain that binds proteins containing E/DFPPPPXD/E motifs and targets Ena-VASP proteins to focal adhesions. In the mid-region of the protein, family members have a proline-rich domain that binds SH3 and WW domain-containing proteins. Their C-terminal EVH2 domain mediates tetramerization and binds both G and F actin. VASP is associated with filamentous actin formation and likely plays a widespread role in cell adhesion and motility. VASP may also be involved in the intracellular signaling pathways that regulate integrin-extracellular matrix interactions. VASP is regulated by the cyclic nucleotide-dependent kinases PKA and PKG.
Zhao WM, et al. (2001) EMBO J 20(9): 2315-2325.
Millard TH, et al. (2005) EMBO J 24(2): 240-250.
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Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A00303 is guaranteed for IF, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Predicted MW: 50kd
Western blotting: 1:500~1:1000
Immunofluorescence: 1:100~1:200
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of VASP using anti-VASP antibody (A00303).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-VASP antigen affinity purified polyclonal antibody (Catalog # A00303) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-Rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # SA1022) with Tanon 5200 system. A specific band was detected for VASP.
Click image to see more details
Immunofluorescence staining of methanol-fixed Hela cells using VASP(Ab-157) Antibody #A00303.
Protein Target Info & Infographic
Gene/Protein Information For VASP (Source: Uniprot.org, NCBI)
Gene Name
VASP
Full Name
Vasodilator-stimulated phosphoprotein
Weight
59257 MW
Superfamily
Ena/VASP family
Alternative Names
Glucose-6-phosphate 1-dehydrogenase;G6PD;1.1.1.49;G6PD; VASP vasodilator stimulated phosphoprotein vasodilator-stimulated phosphoprotein
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on VASP, check out the VASP Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for VASP: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-VASP (Ab-157) Antibody (A00303)
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No publications found for A00303
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Customer Q&As
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16 Customer Q&As for Anti-VASP (Ab-157) Antibody
Question
We purchased anti-VASP (Ab-157) antibody for IF on cervix carcinoma erythroleukemia a few years ago. I am using human, and We want to use the antibody for WB next. Our lab want to know about examining cervix carcinoma erythroleukemia as well as fetal spleen skin in our next experiment. Could you please give me some suggestion on which antibody would work the best for WB?
Verified Customer
Verified customer
Asked: 2020-05-05
Answer
I have checked the website and datasheets of our anti-VASP (Ab-157) antibody and I see that A00303 has been validated on human in both IF and WB. Thus A00303 should work for your application. Our Boster satisfaction guarantee will cover this product for WB in human even if the specific tissue type has not been validated. We do have a comprehensive range of products for WB detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2020-05-05
Question
Is there a BSA free version of anti-VASP (Ab-157) antibody A00303 available?
Verified Customer
Verified customer
Asked: 2020-04-29
Answer
Thanks for your recent telephone inquiry. I can confirm that some lots of this anti-VASP (Ab-157) antibody A00303 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2020-04-29
Question
I was wanting to use your anti-VASP (Ab-157) antibody for IF for human platelet on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human platelet identification?
Verified Customer
Verified customer
Asked: 2020-04-24
Answer
As indicated on the product datasheet, A00303 anti-VASP (Ab-157) antibody has been validated for IF, WB on human, rat tissues. We have an innovator award program that if you test this antibody and show it works in human platelet in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2020-04-24
Question
Is this A00303 anti-VASP (Ab-157) antibody reactive to the isotypes of VASP?
Verified Customer
Verified customer
Asked: 2020-03-31
Answer
The immunogen of A00303 anti-VASP (Ab-157) antibody is Peptide sequence around aa.155~159 (R-V-S-N-A) derived from Human VASP. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2020-03-31
Question
Would A00303 anti-VASP (Ab-157) antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2020-02-17
Answer
As indicated on the product datasheet, A00303 anti-VASP (Ab-157) antibody as been validated on IF. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2020-02-17
Question
I appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for platelet using anti-VASP (Ab-157) antibody A00303. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2020-01-27
Answer
Thank you for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2020-01-27
Question
We have been able to see staining in rat cervix carcinoma. Any tips? Is anti-VASP (Ab-157) antibody supposed to stain cervix carcinoma positively?
Verified Customer
Verified customer
Asked: 2019-07-24
Answer
From what I have seen in literature cervix carcinoma does express VASP. From what I have seen in Uniprot.org, VASP is expressed in blood, promyelocyte, substantia nigra, fetal lung, fetal spleen skin, platelet, cervix carcinoma, leukemic t-cell, cervix carcinoma erythroleukemia, liver, among other tissues. Regarding which tissues have VASP expression, here are a few articles citing expression in various tissues:
Cervix carcinoma, Pubmed ID: 18669648, 20068231
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Fetal lung, Fetal spleen, and Skin, Pubmed ID: 15489334
Leukemic T-cell, Pubmed ID: 19690332
Liver, Pubmed ID: 24275569
Platelet, Pubmed ID: 12665801, 18088087
Promyelocyte, Pubmed ID: 7828592
Substantia nigra, Pubmed ID: 14702039
Boster Scientific Support
Answered: 2019-07-24
Question
We are currently using anti-VASP (Ab-157) antibody A00303 for rat tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human, rat. Is it possible that the antibody can work on dog tissues as well?
Verified Customer
Verified customer
Asked: 2019-06-27
Answer
The anti-VASP (Ab-157) antibody (A00303) has not been tested for cross reactivity specifically with dog tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in dog you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-06-27
Question
We want to test anti-VASP (Ab-157) antibody A00303 on human platelet for research purposes, then I may be interested in using anti-VASP (Ab-157) antibody A00303 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2019-06-10
Answer
The products we sell, including anti-VASP (Ab-157) antibody A00303, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-06-10
Question
See below the WB image, lot number and protocol we used for platelet using anti-VASP (Ab-157) antibody A00303. Please let me know if you require anything else.
R. Li
Verified customer
Asked: 2018-06-05
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2018-06-05
Question
Is a blocking peptide available for product anti-VASP (Ab-157) antibody (A00303)?
Verified Customer
Verified customer
Asked: 2017-11-24
Answer
We do provide the blocking peptide for product anti-VASP (Ab-157) antibody (A00303). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2017-11-24
Question
Does anti-VASP (Ab-157) antibody A00303 work for IF with platelet?
Verified Customer
Verified customer
Asked: 2017-07-13
Answer
According to the expression profile of platelet, VASP is highly expressed in platelet. So, it is likely that anti-VASP (Ab-157) antibody A00303 will work for IF with platelet.
Boster Scientific Support
Answered: 2017-07-13
Question
Can you help my question with product A00303, anti-VASP (Ab-157) antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
R. Zhao
Verified customer
Asked: 2017-01-09
Answer
We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A00303 anti-VASP (Ab-157) antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2017-01-09
Question
I see that the anti-VASP (Ab-157) antibody A00303 works with IF, what is the protocol used to produce the result images on the product page?
A. Huang
Verified customer
Asked: 2015-07-08
Answer
You can find protocols for IF on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2015-07-08
Question
Our team were well pleased with the WB result of your anti-VASP (Ab-157) antibody. However we have seen positive staining in platelet cytoplasm. cytoplasm using this antibody. Is that expected? Could you tell me where is VASP supposed to be expressed?
K. Bhatt
Verified customer
Asked: 2013-11-29
Answer
Based on literature, platelet does express VASP. Generally VASP expresses in cytoplasm. cytoplasm, cytoskeleton. cell. Regarding which tissues have VASP expression, here are a few articles citing expression in various tissues:
Cervix carcinoma, Pubmed ID: 18669648, 20068231
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Fetal lung, Fetal spleen, and Skin, Pubmed ID: 15489334
Leukemic T-cell, Pubmed ID: 19690332
Liver, Pubmed ID: 24275569
Platelet, Pubmed ID: 12665801, 18088087
Promyelocyte, Pubmed ID: 7828592
Substantia nigra, Pubmed ID: 14702039
Boster Scientific Support
Answered: 2013-11-29
Question
My question regards using your anti-VASP (Ab-157) antibody for actin polymerization or depolymerization studies. Has this antibody been tested with western blotting on hela cells? We would like to see some validation images before ordering.
E. Jones
Verified customer
Asked: 2013-08-16
Answer
I appreciate your inquiry. This A00303 anti-VASP (Ab-157) antibody is validated on hela cells. It is guaranteed to work for IF, WB in human, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2013-08-16
