Anti-USP5 Antibody

Product Name

Anti-USP5 Antibody

View all Isopeptidase T/USP5 Antibodies

SKU/Catalog Number

M04550

Size

80 µl

Description

Boster Bio Anti-USP5 Antibody (Catalog # M04550). Tested in WB application(s). This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Maintain refrigerated at 2-8°C for up to 2 weeks. For long-term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Cite This Product

Anti-USP5 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M04550)

Host

Mouse

Contents

Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.

Clonality

Monoclonal

Clone Number

1340CT704.170.140

Isotype

IgG1,κ

Immunogen

This USP5 antibody is generated from a mouse immunized with a recombination protein from the human region of human USP5.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

M04550 is reactive to USP5 in Human, Mouse, Rat

Applications

M04550 is guaranteed for WB Boster Guarantee

Observed Molecular Weight

Calculated molecular weight

95786 Da

Background of Isopeptidase T/USP5

Cleaves linear and branched multiubiquitin polymers with a marked preference for branched polymers. Involved in unanchored 'Lys-48'-linked polyubiquitin disassembly. Binds linear and 'Lys- 63'-linked polyubiquitin with a lower affinity. Knock-down of USP5 causes the accumulation of p53/TP53 and an increase in p53/TP53 transcriptional activity because the unanchored polyubiquitin that accumulates is able to compete with ubiquitinated p53/TP53 but not with MDM2 for proteasomal recognition.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

WB: 1:2000

Validation Images & Assay Conditions

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Western blot analysis of lysates from U251, PC-3 cell line, mouse brain, rat brain and liver tissue lysates (from left to right), using USP5 Antibody. M04550 was diluted at 1:1000 at each lane. A goat anti-mouse IgG H&L (HRP) at 1:3000 dilution was used as the secondary antibody. Lysates at 35μg per lane.

Gene/Protein Information For USP5 (Source: Uniprot.org, NCBI)

Alternative Names

Deubiquitinating enzyme 5; EC 3.1.2.15; EC 3.4.19.12; Isopeptidase T; IsoT; ISOTubiquitin carboxyl-terminal hydrolase 5; ubiquitin isopeptidase T; ubiquitin specific peptidase 5 (isopeptidase T); ubiquitin specific protease 5 (isopeptidase T); ubiquitin thioesterase 5; Ubiquitin thiolesterase 5; ubiquitin-specific protease-5 (ubiquitin isopeptidase T); Ubiquitin-specific-processing protease 5; USP5 USP5 ISOT ubiquitin specific peptidase 5 ubiquitin carboxyl-terminal hydrolase 5|deubiquitinating enzyme 5|isopeptidase T|testicular tissue protein Li 218|ubiquitin isopeptidase T|ubiquitin specific protease 5 (isopeptidase T)|ubiquitin thioesterase 5|ubiquitin thiolesterase 5|ubiquitin-specific protease-5 (ubiquitin isopeptidase T)|ubiquitin-specific-processing protease 5

For more info on USP5, check out the USP5 Infographic

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In this infographic, you will see the following information for USP5: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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