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Product Info Summary
| SKU: | A09878 |
|---|---|
| Size: | 100 µl |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-USP45 Antibody
SKU/Catalog Number
A09878
Size
100 µl
Form
Liquid
Description
Boster Bio Anti-USP45 Antibody (Catalog # A09878). Tested in WB applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-USP45 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A09878)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthesized peptide derived from internal of human USP45.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A09878 is reactive to USP45 in Human
Reconstitution
Calculated molecular weight
91733 MW
Background of Usp45
Belongs to the peptidase C19 family. 3 isoforms of the human protein are produced by alternative splicing.
Quesada V., Biochem. Biophys. Res. Commun. 314:54-62(2004).
Bechtel S., BMC Genomics 8:399-399(2007).
Mungall A.J., Nature 425:805-811(2003).
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Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A09878 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blotting: 1:500~1:3000
Validation Images & Assay Conditions
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Western blot analysis of extracts from A549 cells, using USP45 antibody A09878.
Protein Target Info & Infographic
Gene/Protein Information For USP45 (Source: Uniprot.org, NCBI)
Gene Name
USP45
Full Name
Ubiquitin carboxyl-terminal hydrolase 45
Weight
91733 MW
Superfamily
peptidase C19 family
Alternative Names
Ubiquitin carboxyl-terminal hydrolase 45;3.4.19.12;Deubiquitinating enzyme 45;Ubiquitin thioesterase 45;Ubiquitin-specific-processing protease 45;USP45;
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on USP45, check out the USP45 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for USP45: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-USP45 Antibody (A09878)
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No publications found for A09878
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Customer Reviews
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Customer Q&As
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7 Customer Q&As for Anti-USP45 Antibody
Question
Will A09878 anti-USP45 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2020-03-30
Answer
You can see on the product datasheet, A09878 anti-USP45 antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2020-03-30
Question
I was wanting to use your anti-USP45 antibody for WB for human bone marrow brain cortex on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human bone marrow brain cortex identification?
Verified Customer
Verified customer
Asked: 2020-02-07
Answer
It shows on the product datasheet, A09878 anti-USP45 antibody has been validated for WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human bone marrow brain cortex in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2020-02-07
Question
I see that the anti-USP45 antibody A09878 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-07-08
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2019-07-08
Question
Is a blocking peptide available for product anti-USP45 antibody (A09878)?
B. Walker
Verified customer
Asked: 2018-06-26
Answer
We do provide the blocking peptide for product anti-USP45 antibody (A09878). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2018-06-26
Question
Do you have a BSA free version of anti-USP45 antibody A09878 available?
Verified Customer
Verified customer
Asked: 2018-01-19
Answer
Thank you for your recent telephone inquiry. I can confirm that some lots of this anti-USP45 antibody A09878 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2018-01-19
Question
I have a question about product A09878, anti-USP45 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
N. Jackson
Verified customer
Asked: 2017-06-06
Answer
We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A09878 anti-USP45 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2017-06-06
Question
We are currently using anti-USP45 antibody A09878 for human tissue, and we are content with the WB results. The species of reactivity given in the datasheet says human. Is it likely that the antibody can work on pig tissues as well?
C. Carter
Verified customer
Asked: 2014-10-31
Answer
The anti-USP45 antibody (A09878) has not been tested for cross reactivity specifically with pig tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in pig you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2014-10-31
