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Product Info Summary
| SKU: | M06534 |
|---|---|
| Size: | 100ug/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-Uroplakin 3A (Bladder Carcinoma Marker) Antibody
View all Uroplakin III Antibodies
SKU/Catalog Number
M06534
Size
100ug/vial
Form
Liquid
Description
Boster Bio Anti-Uroplakin 3A (Bladder Carcinoma Marker) Antibody (Catalog # M06534). Tested in WB applications. This antibody reacts with Human.
Storage & Handling
Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
Cite This Product
Anti-Uroplakin 3A (Bladder Carcinoma Marker) Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M06534)
Host
Rabbit
Contents
Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA at 1.0mg/ml.
Clonality
Polyclonal
Isotype
IgG
Immunogen
A synthetic peptide, aa260-279 (GASESSYTSVNRGPPLDRAE) of human UPK3A
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
Does not cross-react with primate, avian or amphibian GR.
Reactive Species
M06534 is reactive to UPK3A in Human
Reconstitution
Reconstitute with distilled water.
Observed Molecular Weight
68 kDa
Calculated molecular weight
30670 MW
Background of Uroplakin III
Uroplakin-3A (UPK3A) is a component of the asymmetric unit membrane (AUM). It is a highly specialized bio-membrane made by terminally differentiated urothelial cells. The protein may play an important role in AUM-cytoskeleton interaction in terminally differentiated urothelial cells. UPK3A also contributes to the formation of urothelial glycocalyx, which may play an important role in preventing bacterial adherence through FimH bacterial protein binding leading to bladder infection. UPK3A has been shown as a helpful marker for the detection of bladder cancer.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M06534 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western Blot (1-2ug/ml)
Optimal dilution for a specific application should be determined.
Validation Images & Assay Conditions
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Western Blot analysis in human HEK293 lysate in 1) absence and 2) presence of immunizing peptide using Anti-UPK3A Rabbit Polyclonal Antibody.
Protein Target Info & Infographic
Gene/Protein Information For UPK3A (Source: Uniprot.org, NCBI)
Gene Name
UPK3A
Full Name
Uroplakin-3a
Weight
30670 MW
Superfamily
uroplakin-3 family
Alternative Names
Uroplakin-3a;UP3a;Uroplakin III;UPIII;UPK3A;UPK3; UPK3A UP3A, UPIII, UPIIIA, UPK3 uroplakin 3A uroplakin-3a|uroplakin 3|uroplakin III
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on UPK3A, check out the UPK3A Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for UPK3A: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Uroplakin 3A (Bladder Carcinoma Marker) Antibody (M06534)
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No publications found for M06534
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Customer Q&As
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3 Customer Q&As for Anti-Uroplakin 3A (Bladder Carcinoma Marker) Antibody
Question
Do you have a BSA free version of anti-Uroplakin 3A (Bladder Carcinoma Marker) antibody M06534 available?
Verified Customer
Verified customer
Asked: 2019-10-16
Answer
I appreciate your recent telephone inquiry. I can confirm that some lots of this anti-Uroplakin 3A (Bladder Carcinoma Marker) antibody M06534 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-10-16
Question
I was wanting to use your anti-Uroplakin 3A (Bladder Carcinoma Marker) antibody for WB for human ureter on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human ureter identification?
Verified Customer
Verified customer
Asked: 2019-07-04
Answer
You can see on the product datasheet, M06534 anti-Uroplakin 3A (Bladder Carcinoma Marker) antibody has been tested for WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human ureter in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-07-04
Question
Does M06534 anti-Uroplakin 3A (Bladder Carcinoma Marker) antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2018-05-04
Answer
It shows on the product datasheet, M06534 anti-Uroplakin 3A (Bladder Carcinoma Marker) antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2018-05-04
