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Product Info Summary
| SKU: | M09436 |
|---|---|
| Size: | 100 μl/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IHC, WB |
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Product info
Product Name
Anti-UQCRH Rabbit Monoclonal Antibody
SKU/Catalog Number
M09436
Size
100 μl/vial
Form
Liquid
Description
Boster Bio Anti-UQCRH Rabbit Monoclonal Antibody catalog # M09436. Tested in WB, IHC applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-UQCRH Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M09436)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
27U84
Isotype
IgG
Immunogen
A synthesized peptide derived from human UQCRH
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M09436 is reactive to UQCRH in Human, Mouse, Rat
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
13 kDa
Calculated molecular weight
26132 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M09436 is guaranteed for IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:50-1:200
Positive Control
WB: human K562 whole cell, human THP-1 whole cell, rat brain tissue, rat stomach tissue, mouse brain tissue, mouse stomach tissue
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of UQCRH using anti-UQCRH antibody (M09436).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human K562 whole cell lysates,
Lane 2: human THP-1 whole cell lysates,
Lane 3: rat brain tissue lysates,
Lane 4: rat stomach tissue lysates,
Lane 5: mouse brain tissue lysates,
Lane 6: mouse stomach tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-UQCRH antigen affinity purified monoclonal antibody (Catalog # M09436) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:500 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for UQCRH at approximately 13 kDa. The expected band size for UQCRH is at 11 kDa.
Protein Target Info & Infographic
Gene/Protein Information For UQCRH (Source: Uniprot.org, NCBI)
Gene Name
UQCRH
Full Name
Cytochrome b-c1 complex subunit 6, mitochondrial
Weight
26132 MW
Superfamily
UQCRH/QCR6 family
Alternative Names
Vesicle transport protein SEC20;BCL2/adenovirus E1B 19 kDa protein-interacting protein 1;Transformation-related gene 8 protein;TRG-8;BNIP1;NIP1, SEC20L;TRG8; UQCRH QCR6, UQCR8 ubiquinol-cytochrome c reductase hinge protein cytochrome b-c1 complex subunit 6, mitochondrial|complex III subunit 6|cytochrome c1 non-heme 11 kDa protein|mitochondrial hinge protein|ubiquinol-cytochrome c reductase complex 11 kDa protein|ubiquinol-cytochrome c reductase, complex III subunit VIII
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on UQCRH, check out the UQCRH Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for UQCRH: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-UQCRH Rabbit Monoclonal Antibody (M09436)
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