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Product Info Summary
| SKU: | M10128 |
|---|---|
| Size: | 100 μl/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-UPP1 Rabbit Monoclonal Antibody
View all Uridine Phosphorylase 1/UPP1 Antibodies
SKU/Catalog Number
M10128
Size
100 μl/vial
Form
Liquid
Description
Boster Bio Anti-UPP1 Rabbit Monoclonal Antibody catalog # M10128. Tested in WB application. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-UPP1 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M10128)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
26U88
Isotype
IgG
Immunogen
A synthesized peptide derived from human UPP1
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M10128 is reactive to UPP1 in Human, Mouse, Rat
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
28 kDa
Calculated molecular weight
28998 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M10128 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
Positive Control
WB: human Hela whole cell, human 293T whole cell, human Jurkat whole cell, human MCF-7 whole cell, rat heart tissue, rat liver tissue, mouse heart tissue, mouse liver tissue
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of UPP1 using anti-UPP1 antibody (M10128).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human 293T whole cell lysates,
Lane 3: human Jurkat whole cell lysates,
Lane 4: human MCF-7 whole cell lysates,
Lane 5: rat heart tissue lysates,
Lane 6: rat liver tissue lysates,
Lane 7: mouse heart tissue lysates,
Lane 8: mouse liver tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-UPP1 antigen affinity purified monoclonal antibody (M10128) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:500 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for UPP1 at approximately 28 kDa. The expected band size for UPP1 is at 34 kDa.
Protein Target Info & Infographic
Gene/Protein Information For UPP1 (Source: Uniprot.org, NCBI)
Gene Name
UPP1
Full Name
Uridine phosphorylase 1
Weight
28998 MW
Superfamily
PNP/UDP phosphorylase family
Alternative Names
Cell division cycle-associated protein 3;Gene-rich cluster protein C8;Trigger of mitotic entry protein 1;TOME-1;CDCA3;C8, GRCC8, TOME1; UPP1 UDRPASE, UP, UPASE, UPP uridine phosphorylase 1 uridine phosphorylase 1|UPase 1|urdPase 1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on UPP1, check out the UPP1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for UPP1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-UPP1 Rabbit Monoclonal Antibody (M10128)
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