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Product Info Summary
| SKU: | A08552 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC-P, WB |
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Product info
Product Name
Anti-ULK4 Antibody
SKU/Catalog Number
A08552
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-ULK4 Antibody (Catalog # A08552). Tested in ELISA, WB, IHC-P, IF applications. This antibody reacts with Human, Mouse.
Storage & Handling
ULK4 antibody can be stored at 4°C for three months and -20°C, stable for up to one year.
Cite This Product
Anti-ULK4 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A08552)
Host
Rabbit
Contents
ULK4 antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
ULK4 antibody was raised against a 16 amino acid peptide near the carboxy terminus of human ULK4. The immunogen is located within amino acids 1120 - 1170 of ULK4.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
ULK4 antibody is human and mouse reactive. Multiple isoforms of ULK4 are known to exist.
Reactive Species
A08552 is reactive to ULK4 in Human, Mouse
Reconstitution
Observed Molecular Weight
68 kDa
Calculated molecular weight
142442 MW
Background of ULK4
ULK4 belongs to the Ser/Thr protein kinase superfamily and plays a role in the ATP-dependent phosphorylation of target proteins (1). Knockout of ULK genes results in a severe defect in the autophagy pathway (2). ULK4, like the other Unc-51-like kinases such as ULK1, ULK2 and ULK3, is highly conserved among eukaryotes (3). ULK4 has been implicated as a gene involved in the development of hydrocephalus (3). This neurologic disorder is being used in animal models to elucidate factors responsible for the excessive accumulation of cerebrospinal fluid in hydrocephalic humans (4).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A08552 is guaranteed for ELISA, IF, IHC-P, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
ULK4 antibody can be used for detection of ULK4 by Western blot at 0.5 μg/ml. Antibody can also be used for Immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in mouse samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Western blot analysis of ULK4 in 3T3 cell lysate with ULK4 antibody at 0.5 μg/ml in (A) the absence and (B) the presence of blocking peptide.
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Immunohistochemistry of ULK4 in human brain tissue with ULK4 antibody at 5 μg/mL.
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Immunofluorescence of ULK4 in human brain tissue with ULK4 antibody at 20 μg/mL.
Protein Target Info & Infographic
Gene/Protein Information For ULK4 (Source: Uniprot.org, NCBI)
Gene Name
ULK4
Full Name
Serine/threonine-protein kinase ULK4
Weight
142442 MW
Superfamily
protein kinase superfamily
Alternative Names
FAM7C1, REC01035Unc-51-like kinase 4 ULK4 FAM7C1, REC01035 unc-51 like kinase 4 serine/threonine-protein kinase ULK4
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on ULK4, check out the ULK4 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for ULK4: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-ULK4 Antibody (A08552)
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