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Product Info Summary
| SKU: | A01865-2 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, Flow Cytometry, WB |
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Product info
Product Name
Anti-Ugt1a1 Antibody Picoband®
View all Glucuronosyltransferase 1A1/UGT1A1 Antibodies
SKU/Catalog Number
A01865-2
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Ugt1a1 Antibody Picoband® catalog # A01865-2. Tested in ELISA, Flow Cytometry, WB applications. This antibody reacts with Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-Ugt1a1 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01865-2)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived mouse Ugt1a1 recombinant protein (Position: H57-D248).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A01865-2 is reactive to Ugt1a1 in Mouse, Rat
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500 µg/ml.
Observed Molecular Weight
55 kDa
Calculated molecular weight
50433 MW
Background of Glucuronosyltransferase 1A1/UGT1A1
UDP-glucuronosyltransferase 1-1 also known as UGT-1A is an enzyme that in humans is encoded by the UGT1A1 gene. Predicted to enable several functions, including glucuronosyltransferase activity; protein dimerization activity; and retinoic acid binding activity. Predicted to be involved in several processes, including biphenyl catabolic process; cellular glucuronidation; and negative regulation of cellular glucuronidation. Is integral component of plasma membrane. Part of endoplasmic reticulum chaperone complex. Is expressed in alimentary system; nervous system; and urinary system. Human ortholog(s) of this gene implicated in several diseases, including bilirubin metabolic disorder (multiple); cholecystolithiasis; cholelithiasis; female reproductive organ cancer (multiple); and hepatitis B. Orthologous to human UGT1A1 (UDP glucuronosyltransferase family 1 member A1).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A01865-2 is guaranteed for ELISA, Flow Cytometry, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5 μg/ml, Mouse, Rat
Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Mouse
ELISA, 0.1-0.5 μg/ml, -
Positive Control
WB: rat liver tissue, rat RH35 whole cell, mouse liver tissue
FCM: ANA-1 cell
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of Ugt1a1 using anti-Ugt1a1 antibody (A01865-2).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: rat liver tissue lysates,
Lane 2: rat RH35 whole cell lysates,
Lane 3: mouse liver tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Ugt1a1 antigen affinity purified polyclonal antibody (Catalog # A01865-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Ugt1a1 at approximately 55 kDa. The expected band size for Ugt1a1 is at 60 kDa.
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Figure 2. Flow Cytometry analysis of ANA-1 cells using anti-Ugt1a1 antibody (A01865-2).
Overlay histogram showing ANA-1 cells stained with A01865-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Ugt1a1 Antibody (A01865-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Protein Target Info & Infographic
Gene/Protein Information For Ugt1a1 (Source: Uniprot.org, NCBI)
Gene Name
Ugt1a1
Full Name
UDP-glucuronosyltransferase 1-1
Weight
50433 MW
Superfamily
UDP-glycosyltransferase family
Alternative Names
UDP-glucuronosyltransferase 1-1; UDPGT 1-1; UGT1*1; UGT1-01; UGT1.1; Bilirubin-specific UDPGT isozyme 1; hUG-BR1; UDP-glucuronosyltransferase 1-A; UGT-1A; UGT1A; UDP-glucuronosyltransferase 1A1; UGT1A1; GNT1; UGT1 Ugt1a1|UDPGT 1-1, Udpgt, Ugt1|UDP glucuronosyltransferase family 1 member A1|UDP-glucuronosyltransferase 1A1|B1|UDP glucuronosyltransferase 1 family, polypeptide A1|UDP glycosyltransferase 1 family, polypeptide A1|UDP-glucuronosyltransferase 1 family member 1|UDP-glucuronosyltransferase 1-1|UGT1*1|UGT1-01|UGT1.1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on Ugt1a1, check out the Ugt1a1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for Ugt1a1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Ugt1a1 Antibody Picoband® (A01865-2)
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