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Product Info Summary
| SKU: | A04764 |
|---|---|
| Size: | 100 µl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-UGDH/Udp Glcdh Antibody
View all UDP glucose dehydrogenase Antibodies
SKU/Catalog Number
A04764
Size
100 µl
Form
Liquid
Description
Boster Bio Anti-UGDH/Udp Glcdh Antibody (Catalog # A04764). Tested in WB applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-UGDH/Udp Glcdh Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A04764)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthesized peptide derived from internal of human UGDH.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A04764 is reactive to UGDH in Human, Mouse, Rat
Reconstitution
Calculated molecular weight
55024 MW
Background of UDP glucose dehydrogenase
Involved in the biosynthesis of glycosaminoglycans; hyaluronan, chondroitin sulfate, and heparan sulfate.
Spicer A.P., J. Biol. Chem. 273:25117-25124(1998).
Peng H.L., Proc. Natl. Sci. Counc. Repub. China, B, Life Sci. 22:166-172(1998).
Chang M.L., Submitted (FEB-2000) to the EMBL/GenBank/DDBJ databases.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A04764 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blotting: 1:500~1:3000
Validation Images & Assay Conditions
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Western blot analysis of extracts from COLO cells, using UGDH antibody A04764.
Protein Target Info & Infographic
Gene/Protein Information For UGDH (Source: Uniprot.org, NCBI)
Gene Name
UGDH
Full Name
UDP-glucose 6-dehydrogenase
Weight
55024 MW
Superfamily
UDP-glucose/GDP-mannose dehydrogenase family
Alternative Names
UDP-glucose 6-dehydrogenase;UDP-Glc dehydrogenase;UDP-GlcDH;UDPGDH;1.1.1.22;UGDH; UGDH DEE84, EIEE84, GDH, UDP-GlcDH, UDPGDH, UGD UDP-glucose 6-dehydrogenase UDP-glucose 6-dehydrogenase|UDP-Glc dehydrogenase|UDP-glucose dehydrogenase|uridine diphospho-glucose dehydrogenase
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on UGDH, check out the UGDH Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for UGDH: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-UGDH/Udp Glcdh Antibody (A04764)
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No publications found for A04764
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Customer Reviews
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Customer Q&As
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13 Customer Q&As for Anti-UGDH/Udp Glcdh Antibody
Question
Will anti-UGDH/Udp Glcdh antibody A04764 work for WB with thymus?
Verified Customer
Verified customer
Asked: 2020-01-31
Answer
According to the expression profile of thymus, UGDH is highly expressed in thymus. So, it is likely that anti-UGDH/Udp Glcdh antibody A04764 will work for WB with thymus.
Boster Scientific Support
Answered: 2020-01-31
Question
I was wanting to use your anti-UGDH/Udp Glcdh antibody for WB for mouse thymus on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for mouse thymus identification?
Verified Customer
Verified customer
Asked: 2020-01-08
Answer
It shows on the product datasheet, A04764 anti-UGDH/Udp Glcdh antibody has been validated for WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in mouse thymus in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2020-01-08
Question
Is there a BSA free version of anti-UGDH/Udp Glcdh antibody A04764 available?
Verified Customer
Verified customer
Asked: 2019-12-20
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-UGDH/Udp Glcdh antibody A04764 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-12-20
Question
Is a blocking peptide available for product anti-UGDH/Udp Glcdh antibody (A04764)?
K. Li
Verified customer
Asked: 2019-08-21
Answer
We do provide the blocking peptide for product anti-UGDH/Udp Glcdh antibody (A04764). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2019-08-21
Question
Thanks for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for thymus using anti-UGDH/Udp Glcdh antibody A04764. Let me know if you need anything else.
K. Brown
Verified customer
Asked: 2019-08-07
Answer
Thanks for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-08-07
Question
Would anti-UGDH/Udp Glcdh antibody A04764 work on primate WB with cervix carcinoma?
Verified Customer
Verified customer
Asked: 2019-07-25
Answer
Our lab technicians have not validated anti-UGDH/Udp Glcdh antibody A04764 on primate. You can run a BLAST between primate and the immunogen sequence of anti-UGDH/Udp Glcdh antibody A04764 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated primate samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in primate cervix carcinoma in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-07-25
Question
My question regarding product A04764, anti-UGDH/Udp Glcdh antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-07-01
Answer
We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A04764 anti-UGDH/Udp Glcdh antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-07-01
Question
Does A04764 anti-UGDH/Udp Glcdh antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-06-14
Answer
As indicated on the product datasheet, A04764 anti-UGDH/Udp Glcdh antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-06-14
Question
We need to test anti-UGDH/Udp Glcdh antibody A04764 on mouse thymus for research purposes, then I may be interested in using anti-UGDH/Udp Glcdh antibody A04764 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2019-06-03
Answer
The products we sell, including anti-UGDH/Udp Glcdh antibody A04764, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-06-03
Question
Please see the WB image, lot number and protocol we used for thymus using anti-UGDH/Udp Glcdh antibody A04764. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2018-07-05
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2018-07-05
Question
Is this A04764 anti-UGDH/Udp Glcdh antibody reactive to the isotypes of UGDH?
Verified Customer
Verified customer
Asked: 2017-10-17
Answer
The immunogen of A04764 anti-UGDH/Udp Glcdh antibody is Synthesized peptide derived from internal of human UGDH. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2017-10-17
Question
We are currently using anti-UGDH/Udp Glcdh antibody A04764 for human tissue, and we are content with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on dog tissues as well?
Verified Customer
Verified customer
Asked: 2017-05-12
Answer
The anti-UGDH/Udp Glcdh antibody (A04764) has not been validated for cross reactivity specifically with dog tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in dog you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2017-05-12
Question
I see that the anti-UGDH/Udp Glcdh antibody A04764 works with WB, what is the protocol used to produce the result images on the product page?
J. Baker
Verified customer
Asked: 2015-06-04
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2015-06-04
