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Product Info Summary
| SKU: | M00582 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-UBE3A Monoclonal Antibody
View all E6AP/UBE3A Antibodies
SKU/Catalog Number
M00582
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-UBE3A Monoclonal Antibody catalog # M00582. Tested in WB application. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-UBE3A Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00582)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
ADBA-21
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human UBE3A E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and transfers it to its substrates. Several substrates have been identified including the RAD23A and RAD23B, MCM7 (which is involved in DNA replication), annexin A1, the PML tumor suppressor, and the cell cycle regulator CDKN1B.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M00582 is reactive to UBE3A in Human, Mouse, Rat
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
47 kDa
Calculated molecular weight
36502 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00582 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
Validation Images & Assay Conditions
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Western blot analysis of UBE3A expression in K562 cell lysate.
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All lanes use the Antibody at 1:1W dilution for 1 hour at room temperature.
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All lanes use the Antibody at 1:1W dilution for 1 hour at room temperature.
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All lanes use the Antibody at 1:1W dilution for 1 hour at room temperature.
Protein Target Info & Infographic
Gene/Protein Information For UBE3A (Source: Uniprot.org, NCBI)
Gene Name
UBE3A
Full Name
Ubiquitin-protein ligase E3A
Weight
36502 MW
Alternative Names
Interferon regulatory factor 1;IRF-1;IRF1; UBE3A ANCR, AS, E6-AP, EPVE6AP, HPVE6A ubiquitin protein ligase E3A ubiquitin-protein ligase E3A|CTCL tumor se37-2|E6AP ubiquitin-protein ligase|HECT-type ubiquitin transferase E3A|human papilloma virus E6-associated protein|human papillomavirus E6-associated protein|oncogenic protein-associated protein E6-AP|renal carcinoma NY-REN-54
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on UBE3A, check out the UBE3A Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for UBE3A: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-UBE3A Monoclonal Antibody (M00582)
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1 Customer Q&As for Anti-UBE3A Monoclonal Antibody
Question
We are currently using anti-UBE3A Monoclonal antibody M00582 for mouse tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on monkey tissues as well?
Verified Customer
Verified customer
Asked: 2019-08-23
Answer
The anti-UBE3A Monoclonal antibody (M00582) has not been tested for cross reactivity specifically with monkey tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in monkey you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-08-23
