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Product Info Summary
| SKU: | A07183-3 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | ELISA, Flow Cytometry, IF, ICC, WB |
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Product info
Product Name
Anti-UBAP2L Antibody Picoband®
SKU/Catalog Number
A07183-3
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-UBAP2L Antibody Picoband® catalog # A07183-3. Tested in ELISA, Flow Cytometry, IF, ICC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-UBAP2L Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A07183-3)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human UBAP2L recombinant protein (Position: A271-S439).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A07183-3 is reactive to UBAP2L in Human
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Observed Molecular Weight
150 kDa
Calculated molecular weight
25503 MW
Background of UBAP2L
Ubiquitin-associated protein 2-like is a protein that in humans is encoded by the UBAP2L gene. Ubiquitin-associated protein 2-like (UBAP2L), which contains a ubiquitin-associated (UBA) domain near its N-terminus, has been indicated in the pathogenesis of several human cancers, including multiple myeloma, hepatocellular carcinoma, and malignant ovarian tumors. UBAP2L is closely associated with tumor growth and metastasis. UBAP2L has a key role in glioma cell growth and may act as an oncogene to promote malignant glioma development. UBAP2L also plays an important role in breast cancer cell proliferation and might serve as a potential target for breast cancer treatment. UBAP2L plays a critical role in the maintenance of the metastatic ability of hepatocellular carcinoma (HCC) cells via SNAIL1 Regulation and is predictive of a poor clinical outcome.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A07183-3 is guaranteed for ELISA, Flow Cytometry, IF, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.25 μg/ml, Human
Immunocytochemistry/Immunofluorescence, 5 μg/ml, Human
Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human
ELISA, 0.1-0.5 μg/ml, -
Positive Control
WB: human Jurkat whole cell, human K562 whole cell, human PC-3 whole cell, human Hela whole cell
ICC/IF: T-47D cell
FCM: THP-1 cell
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of UBAP2L using anti-UBAP2L antibody (A07183-3).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Jurkat whole cell lysates,
Lane 2: human K562 whole cell lysates,
Lane 3: human PC-3 whole cell lysates,
Lane 4: human Hela whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-UBAP2L antigen affinity purified polyclonal antibody (Catalog # A07183-3) at 0.25 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for UBAP2L at approximately 150 kDa. The expected band size for UBAP2L is at 150 kDa.
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Figure 2. IF analysis of UBAP2L using anti-UBAP2L antibody (A07183-3).
UBAP2L was detected in an immunocytochemical section of T-47D cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-UBAP2L Antibody (A07183-3) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
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Figure 3. Flow Cytometry analysis of THP-1 cells using anti-UBAP2L antibody (A07183-3).
Overlay histogram showing THP-1 cells stained with A07183-3 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-UBAP2L Antibody (A07183-3, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Protein Target Info & Infographic
Gene/Protein Information For UBAP2L (Source: Uniprot.org, NCBI)
Gene Name
UBAP2L
Full Name
Ubiquitin-associated protein 2-like
Weight
25503 MW
Alternative Names
Gastrotropin; GT; Fatty acid-binding protein 6; Ileal lipid-binding protein; ILBP; Intestinal 15 kDa protein; I-15P; Intestinal bile acid-binding protein; I-BABP; FABP6; ILBP; ILLBP UBAP2L NICE-4, NICE4 ubiquitin associated protein 2 like ubiquitin-associated protein 2-like|protein NICE-4
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on UBAP2L, check out the UBAP2L Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for UBAP2L: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-UBAP2L Antibody Picoband® (A07183-3)
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