Anti-TWIST1/2 Antibody Picoband®

TWIST1/2 antibody

Boster Bio Anti-TWIST1/2 Antibody Picoband® catalog # A00980-1. Tested in Flow Cytometry, IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Product Info Summary

SKU: A00980-1
Size: 100 μg/vial
Reactive Species: Human, Mouse, Rat
Host: Rabbit
Application: Flow Cytometry, IHC, WB

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Product Name

Anti-TWIST1/2 Antibody Picoband®

View all TWIST1/2 Antibodies

SKU/Catalog Number

A00980-1

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-TWIST1/2 Antibody Picoband® catalog # A00980-1. Tested in Flow Cytometry, IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Storage & Handling

At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Cite This Product

Anti-TWIST1/2 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00980-1)

Host

Rabbit

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

A synthetic peptide corresponding to a sequence in the middle region of human TWIST1/2, identical to the related mouse sequences.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins.

Reactive Species

A00980-1 is reactive to TWIST1/2 in Human, Mouse, Rat

Reconstitution

Adding 0.2 ml of distilled water will yield a concentration of 500 µg/ml.

Observed Molecular Weight

30 kDa

Background of TWIST1/2

Twist-related protein 1 (TWIST1) also known as class A basic helix–loop–helix protein 38 (bHLHa38) is a basic helix-loop-helix transcription factor that in humans is encoded by the TWIST1 gene. This gene encodes a basic helix-loop-helix (bHLH) transcription factor that plays an important role in embryonic development. The encoded protein forms both homodimers and heterodimers that bind to DNA E box sequences and regulate the transcription of genes involved in cranial suture closure during skull development. This protein may also regulate neural tube closure, limb development and brown fat metabolism. This gene is hypermethylated and overexpressed in multiple human cancers, and the encoded protein promotes tumor cell invasion and metastasis, as well as metastatic recurrence. Mutations in this gene cause Saethre-Chotzen syndrome in human patients, which is characterized by craniosynostosis, ptosis and hypertelorism.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

A00980-1 is guaranteed for Flow Cytometry, IHC, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.25-0.5 µg/ml, Mouse, Rat
Immunohistochemistry(Paraffin-embedded Section), 2-5 µg/ml, Human, Mouse, Rat
Flow Cytometry (Fixed), 1-3 µg/1x106 cells, Human, Mouse, Rat

Positive Control

WB: rat brain tissue, rat ovary tissue, mouse brain tissue, mouse ovary tissue
IHC: human penis squamous cell carcinoma tissue, human lung squamous cell carcinoma tissue, human lung cancer tissue, mouse brain tissue, rat brain tissue
FCM: THP-1 cell, RAW2647 cell, RH35 cell

Validation Images & Assay Conditions

Gene/Protein Information For TWIST1/2 (Source: Uniprot.org, NCBI)

Gene Name

TWIST1/2

Full Name

Weight

Alternative Names

ATP-dependent Clp protease ATP-binding subunit clpX-like, mitochondrial; CLPX

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on TWIST1/2, check out the TWIST1/2 Infographic

TWIST1/2 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for TWIST1/2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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Order DetailsPrice
A00980-1

100μg

$370
A00980-1-10ug

10μg sample (liquid)

$99
A00980-1-Biotin

100 μg Biotin conjugated

$570
A00980-1-Cy3

100 μg Cy3 conjugated

$570
A00980-1-Dylight488

100 μg Dylight488 conjugated

$570
A00980-1-Dylight550

100 μg Dylight550 conjugated

$570
A00980-1-Dylight594

100 μg Dylight594 conjugated

$570
A00980-1-FITC

100 μg FITC conjugated

$570
A00980-1-HRP

100 μg HRP conjugated

$570
A00980-1-APC

100 μg APC conjugated

$670
A00980-1-PE

100 μg PE conjugated

$670
A00980-1-iFluor647

100 μg iFluor647 conjugated

$670
A00980-1-carrier-free

Carrier Free

$370

More conjugates/formats

Free Secondary Antibody

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A00980-1
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00

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