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Product Info Summary
| SKU: | A15165 |
|---|---|
| Size: | 100μl |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-TWIK-3 Antibody
SKU/Catalog Number
A15165
Size
100μl
Form
Liquid
Description
Boster Bio Anti-TWIK-3 Antibody catalog # A15165. Tested in WB applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-TWIK-3 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A15165)
Host
Rabbit
Contents
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthesized peptide derived from TWIK-3 . at AA range: 170-250
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A15165 is reactive to KCNK7 in Human
Reconstitution
Calculated molecular weight
31947 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A15165 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB, 1:500-1:2000
Validation Images & Assay Conditions
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Western Blot (WB) analysis of JK cells using TWIK-3 Polyclonal antibody.
Protein Target Info & Infographic
Gene/Protein Information For KCNK7 (Source: Uniprot.org, NCBI)
Gene Name
KCNK7
Full Name
Potassium channel subfamily K member 7
Weight
31947 MW
Superfamily
two pore domain potassium channel (TC 1.A.1.8) family
Alternative Names
Potassium channel subfamily K member 7;KCNK7; KCNK7 K2p7.1, TWIK3 potassium two pore domain channel subfamily K member 7 potassium channel subfamily K member 7|potassium channel, two pore domain subfamily K, member 7|two pore domain K+ channel
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on KCNK7, check out the KCNK7 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for KCNK7: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-TWIK-3 Antibody (A15165)
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No publications found for A15165
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Customer Q&As
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4 Customer Q&As for Anti-TWIK-3 Antibody
Question
We are currently using anti-TWIK-3 antibody A15165 for human tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says human. Is it true that the antibody can work on primate tissues as well?
Verified Customer
Verified customer
Asked: 2020-05-08
Answer
The anti-TWIK-3 antibody (A15165) has not been tested for cross reactivity specifically with primate tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in primate you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-05-08
Question
Can you help my question with product A15165, anti-TWIK-3 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2018-11-21
Answer
It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A15165 anti-TWIK-3 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2018-11-21
Question
Does A15165 anti-TWIK-3 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
G. Mangal
Verified customer
Asked: 2018-06-07
Answer
It shows on the product datasheet, A15165 anti-TWIK-3 antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2018-06-07
Question
you antibody to test anti-TWIK-3 antibody A15165 on human brain for research purposes, then I may be interested in using anti-TWIK-3 antibody A15165 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2017-09-25
Answer
The products we sell, including anti-TWIK-3 antibody A15165, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2017-09-25
