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Product Info Summary
| SKU: | M06261 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IP, IHC, WB |
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Product info
Product Name
Anti-TWEAKR Rabbit Monoclonal Antibody
View all TWEAK R/TNFRSF12 Antibodies
SKU/Catalog Number
M06261
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-TWEAKR Rabbit Monoclonal Antibody catalog # M06261. Tested in WB, IHC, IP applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-TWEAKR Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M06261)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
GFF-20
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human TWEAKR
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M06261 is reactive to TNFRSF12A in Human, Mouse, Rat
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
17,20 kDa
Calculated molecular weight
17,20 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M06261 is guaranteed for IP, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:1000-1:2000
IHC 1:50-1:200
IP 1:50
Positive Control
WB: human HUVEC whole cell, human A549 whole cell, rat PC-12 whole cell, rat C6 whole cell, mouse NIH/3T3 whole cell, mouse 4T1 whole cell
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of TWEAKR using anti-TWEAKR antibody (M06261).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human HUVEC whole cell lysates,
Lane 2: human A549 whole cell lysates,
Lane 3: rat PC-12 whole cell lysates,
Lane 4: rat C6 whole cell lysates,
Lane 5: mouse NIH/3T3 whole cell lysates,
Lane 6: mouse 4T1 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TWEAKR antigen affinity purified monoclonal antibody (Catalog # M06261) at 1:1000 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:500 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TWEAKR at approximately 17,20 kDa. The expected band size for TWEAKR is at 17,20 kDa.
Protein Target Info & Infographic
Gene/Protein Information For TNFRSF12A (Source: Uniprot.org, NCBI)
Gene Name
TNFRSF12A
Full Name
Tumor necrosis factor receptor superfamily member 12A
Weight
17,20 kDa
Alternative Names
Tumor necrosis factor receptor superfamily member 12A;Fibroblast growth factor-inducible immediate-early response protein 14;FGF-inducible 14;Tweak-receptor;TweakR;CD266;TNFRSF12A;FN14; Tnfrsf12a|AI255180, C87282, Fn1, Fn14, HPIP, TWEAK, TWEAK-R, Twea, TweakR|tumor necrosis factor receptor superfamily, member 12a|tumor necrosis factor receptor superfamily member 12A|FGF-inducible 14|fibroblast growth factor-inducible immediate-early response protein 14|fibroblast growth factor-regulated protein 2|tweak-receptor|type I transmembrane protein Fn14
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on TNFRSF12A, check out the TNFRSF12A Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for TNFRSF12A: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-TWEAKR Rabbit Monoclonal Antibody (M06261)
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Customer Q&As
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5 Customer Q&As for Anti-TWEAKR Rabbit Monoclonal Antibody
Question
We are currently using anti-TWEAKR Rabbit Monoclonal antibody M06261 for mouse tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on primate tissues as well?
Verified Customer
Verified customer
Asked: 2019-10-18
Answer
The anti-TWEAKR Rabbit Monoclonal antibody (M06261) has not been tested for cross reactivity specifically with primate tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in primate you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-10-18
Question
Does anti-TWEAKR Rabbit Monoclonal antibody M06261 work for WB with cortex of kidney?
Verified Customer
Verified customer
Asked: 2019-07-30
Answer
According to the expression profile of cortex of kidney, TNFRSF12A is highly expressed in cortex of kidney. So, it is likely that anti-TWEAKR Rabbit Monoclonal antibody M06261 will work for WB with cortex of kidney.
Boster Scientific Support
Answered: 2019-07-30
Question
Will M06261 anti-TWEAKR Rabbit Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-05-16
Answer
It shows on the product datasheet, M06261 anti-TWEAKR Rabbit Monoclonal antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-05-16
Question
Would anti-TWEAKR Rabbit Monoclonal antibody M06261 work on dog WB with placenta?
J. Baker
Verified customer
Asked: 2014-08-28
Answer
Our lab technicians have not validated anti-TWEAKR Rabbit Monoclonal antibody M06261 on dog. You can run a BLAST between dog and the immunogen sequence of anti-TWEAKR Rabbit Monoclonal antibody M06261 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated dog samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in dog placenta in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2014-08-28
Question
I was wanting to use your anti-TWEAKR Rabbit Monoclonal antibody for WB for rat cortex of kidney on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for rat cortex of kidney identification?
R. Carter
Verified customer
Asked: 2013-02-26
Answer
As indicated on the product datasheet, M06261 anti-TWEAKR Rabbit Monoclonal antibody has been tested for IP, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in rat cortex of kidney in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2013-02-26
