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Product Info Summary
| SKU: | A05606 |
|---|---|
| Size: | 100 µl |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | IHC, WB |
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Product info
Product Name
Anti-TUFM Antibody
SKU/Catalog Number
A05606
Size
100 µl
Form
Liquid
Description
Boster Bio Anti-TUFM Antibody (Catalog # A05606). Tested in WB, IHC applications. This antibody reacts with Human, Mouse.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-TUFM Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A05606)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthesized peptide derived from internal of human TUFM.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A05606 is reactive to TUFM in Human, Mouse
Reconstitution
Observed Molecular Weight
111 kDa
Calculated molecular weight
49542 MW
Background of TUFM
This protein promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis.
Woriax V.L., Biochim. Biophys. Acta 1264:347-356(1995).
Ling M., Gene 197:325-336(1997).
Wells J., FEBS Lett. 358:119-125(1995).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A05606 is guaranteed for IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blotting: 1:500~1:3000
Immunohistochemistry: 1:50~1:100
Validation Images & Assay Conditions
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Western blot analysis of extracts from LOVO cells and 3T3 cells, using TUFM antiobdy A05606.
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Immunohistochemistry analysis of paraffin-embedded human lung carcinoma tissue using TUFM antibody A05606.
Protein Target Info & Infographic
Gene/Protein Information For TUFM (Source: Uniprot.org, NCBI)
Gene Name
TUFM
Full Name
Elongation factor Tu, mitochondrial
Weight
49542 MW
Superfamily
TRAFAC class translation factor GTPase superfamily
Alternative Names
Elongation factor Tu, mitochondrial;EF-Tu;P43;TUFM; TUFM COXPD4, EF-TuMT, EFTU, P43 Tu translation elongation factor, mitochondrial elongation factor Tu, mitochondrial|EF-Tu|epididymis secretory sperm binding protein
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on TUFM, check out the TUFM Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for TUFM: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-TUFM Antibody (A05606)
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No publications found for A05606
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Customer Reviews
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Customer Q&As
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6 Customer Q&As for Anti-TUFM Antibody
Question
I see that the anti-TUFM antibody A05606 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2020-01-20
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2020-01-20
Question
I was wanting to use your anti-TUFM antibody for WB for human testis on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human testis identification?
Verified Customer
Verified customer
Asked: 2019-07-25
Answer
It shows on the product datasheet, A05606 anti-TUFM antibody has been validated for IHC, WB on human, mouse tissues. We have an innovator award program that if you test this antibody and show it works in human testis in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-07-25
Question
Would anti-TUFM antibody A05606 work on goat WB with heart kidney?
Verified Customer
Verified customer
Asked: 2019-07-19
Answer
Our lab technicians have not validated anti-TUFM antibody A05606 on goat. You can run a BLAST between goat and the immunogen sequence of anti-TUFM antibody A05606 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated goat samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in goat heart kidney in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-07-19
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for testis using anti-TUFM antibody A05606. Let me know if you need anything else.
W. Evans
Verified customer
Asked: 2018-10-10
Answer
Thanks for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2018-10-10
Question
Is this A05606 anti-TUFM antibody reactive to the isotypes of TUFM?
J. Patel
Verified customer
Asked: 2016-04-22
Answer
The immunogen of A05606 anti-TUFM antibody is Synthesized peptide derived from internal of human TUFM. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2016-04-22
Question
Here is the WB image, lot number and protocol we used for testis using anti-TUFM antibody A05606. Please let me know if you require anything else.
A. Jackson
Verified customer
Asked: 2015-09-09
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2015-09-09
