Product Info Summary
SKU: | A13852-1 |
---|---|
Size: | 100 µg/vial |
Reactive Species: | Human, Mouse, Rat |
Host: | Rabbit |
Application: | ELISA, Flow Cytometry, WB |
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Product info
Product Name
Anti-TTC39B Antibody Picoband™
SKU/Catalog Number
A13852-1
Size
100 µg/vial
Form
Lyophilized
Description
Boster Bio Anti-TTC39B Antibody Picoband™ catalog # A13852-1. Tested in ELISA, WB, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-TTC39B Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A13852-1)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human TTC39B recombinant protein (Position: K129-D682).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
A13852-1 is reactive to Ttc39b in Human, Mouse, Rat
Applications
A13852-1 is guaranteed for ELISA, Flow Cytometry, WB Boster Guarantee
Observed Molecular Weight
70 kDa
Calculated molecular weight
64099 MW
Background of Ttc39b
Tetratricopeptide repeat protein 39B is a protein that in humans is encoded by the TTC39B gene. TTC39B protein contains two TPR repeats (aa 39 3-426 and 626 -659). TCC39B protein is the product of a high density lipoprotein (HDL) gene that promotes the ubiquitination and degradation of liver X receptor (LXR). TTC39B null or deficient mice challenged with high fat/cholesterol/bile salt diet exhibit increased LX R protein and target gene expression. TTC39B deficiency is reported to stabilize LXR by reducing its polyubiquitination and proteasomal degradation. Hence it reduces the incidence of atherosclerosis and steatohepatitis. TTC39B deficiency mice display higher levels of HDL and Apoplipoprotein A-1 and lower levels of hepatic triglyceride synthesis.However it does not affect glucose tolerance or hepatic gluconeogenesis.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Assay dilution & Images
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500 µg/ml.
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5 µg/ml, Human, Mouse, Rat
Flow Cytometry (Fixed), 1-3 µg/1x106 cells, Human
Direct ELISA, 0.1-0.5 µg/ml, Human
Validation Images & Assay Conditions
![a13852 1 ttc39b primary antibodies wb testing 1 a13852 1 ttc39b primary antibodies wb testing 1](https://www.bosterbio.com/media/catalog/product/a/1/a13852-1-ttc39b-primary-antibodies-wb-testing-1.jpg)
Click image to see more details
Figure 1. Western blot analysis of TTC39B using anti-TTC39B antibody (A13852-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human 293T whole cell lysates,
Lane 2: human Raji whole cell lysates,
Lane 3: human K562 whole cell lysates,
Lane 4: rat testis tissue lysates,
Lane 5: mouse testis tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TTC39B antigen affinity purified polyclonal antibody (Catalog # A13852-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TTC39B at approximately 70 kDa. The expected band size for TTC39B is at 70 kDa.
![a13852 1 ttc39b primary antibodies fcm testing 2 a13852 1 ttc39b primary antibodies fcm testing 2](https://www.bosterbio.com/media/catalog/product/a/1/a13852-1-ttc39b-primary-antibodies-fcm-testing-2.png)
Click image to see more details
Figure 2. Flow Cytometry analysis of HepG2 cells using anti-TTC39B antibody (A13852-1).
Overlay histogram showing HepG2 cells stained with A13852-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-TTC39B Antibody (A13852-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
Protein Target Info & Infographic
Gene/Protein Information For Ttc39b (Source: Uniprot.org, NCBI)
Gene Name
Ttc39b
Full Name
Tetratricopeptide repeat protein 39B
Weight
64099 MW
Superfamily
TTC39 family
Alternative Names
C9orf52; chromosome 9 open reading frame 52; FLJ33868; tetratricopeptide repeat domain 39B; tetratricopeptide repeat protein 39B; TPR repeat protein 39B
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on Ttc39b, check out the Ttc39b Infographic
![Ttc39b infographic](/media/images/gene-infographic-example.jpg)
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for Ttc39b: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-TTC39B Antibody Picoband™ (A13852-1)
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