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Product Info Summary
| SKU: | A15453 |
|---|---|
| Size: | 100 µl |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-TTC23 Antibody
SKU/Catalog Number
A15453
Size
100 µl
Form
Liquid
Description
Boster Bio Anti-TTC23 Antibody (Catalog # A15453). Tested in WB applications. This antibody reacts with Human, Mouse.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-TTC23 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A15453)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthesized peptide derived from internal of human TTC23.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A15453 is reactive to TTC23 in Human, Mouse
Reconstitution
Calculated molecular weight
50009 MW
Background of TTC23
3 isoforms of the human protein are produced by alternative splicing.
Kim J.W., Submitted (AUG-2001) to the EMBL/GenBank/DDBJ databases.
Ota T., Nat. Genet. 36:40-45(2004).
The MGC Project Team, Genome Res. 14:2121-2127(2004).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A15453 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blotting: 1:500~1:3000
Validation Images & Assay Conditions
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Western blot analysis of extracts from RAW264.7 cells, using TTC23 antibody A15453.
Protein Target Info & Infographic
Gene/Protein Information For TTC23 (Source: Uniprot.org, NCBI)
Gene Name
TTC23
Full Name
Tetratricopeptide repeat protein 23
Weight
50009 MW
Alternative Names
Tetratricopeptide repeat protein 23;TPR repeat protein 23;Cervical cancer proto-oncogene 8 protein;HCC-8;TTC23;HCC8; TTC23 HCC-8 tetratricopeptide repeat domain 23 tetratricopeptide repeat protein 23|TPR repeat protein 23|cervical cancer proto-oncogene 8 protein
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on TTC23, check out the TTC23 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for TTC23: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-TTC23 Antibody (A15453)
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No publications found for A15453
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Customer Reviews
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Customer Q&As
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7 Customer Q&As for Anti-TTC23 Antibody
Question
Is this A15453 anti-TTC23 antibody reactive to the isotypes of TTC23?
Verified Customer
Verified customer
Asked: 2020-04-09
Answer
The immunogen of A15453 anti-TTC23 antibody is Synthesized peptide derived from internal of human TTC23. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2020-04-09
Question
Does anti-TTC23 antibody A15453 work on dog WB with colon?
Verified Customer
Verified customer
Asked: 2019-11-25
Answer
Our lab technicians have not tested anti-TTC23 antibody A15453 on dog. You can run a BLAST between dog and the immunogen sequence of anti-TTC23 antibody A15453 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated dog samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in dog colon in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-11-25
Question
I have a question about product A15453, anti-TTC23 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-10-21
Answer
We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A15453 anti-TTC23 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-10-21
Question
Would A15453 anti-TTC23 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-08-28
Answer
It shows on the product datasheet, A15453 anti-TTC23 antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-08-28
Question
I am looking for to test anti-TTC23 antibody A15453 on mouse colon for research purposes, then I may be interested in using anti-TTC23 antibody A15453 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2019-07-11
Answer
The products we sell, including anti-TTC23 antibody A15453, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-07-11
Question
I was wanting to use your anti-TTC23 antibody for WB for mouse colon on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for mouse colon identification?
T. Gonzalez
Verified customer
Asked: 2017-11-10
Answer
As indicated on the product datasheet, A15453 anti-TTC23 antibody has been tested for WB on human, mouse tissues. We have an innovator award program that if you test this antibody and show it works in mouse colon in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2017-11-10
Question
We are currently using anti-TTC23 antibody A15453 for mouse tissue, and we are content with the WB results. The species of reactivity given in the datasheet says human, mouse. Is it likely that the antibody can work on dog tissues as well?
L. Gonzalez
Verified customer
Asked: 2016-06-21
Answer
The anti-TTC23 antibody (A15453) has not been tested for cross reactivity specifically with dog tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in dog you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2016-06-21
