Product Info Summary
SKU: | A01233-1 |
---|---|
Size: | 100ul |
Reactive Species: | Human, Mouse, Rat |
Host: | Rabbit |
Application: | Flow Cytometry, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-TSG101 Antibody
SKU/Catalog Number
A01233-1
Size
100ul
Form
Liquid
Description
Boster Bio Anti-TSG101 Antibody catalog # A01233-1. Tested in WB,ICC/IF,IHC,Flow Cytometry applications. This antibody reacts with Human,Mouse,Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-TSG101 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01233-1)
Host
Rabbit
Contents
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthesized peptide derived from human APLP2 around the phosphorylation site of Y755.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
A01233-1 is reactive to TSG101 in Human, Mouse, Rat
Applications
A01233-1 is guaranteed for Flow Cytometry, IF, IHC, ICC, WB Boster Guarantee
Observed Molecular Weight
Calculated molecular weight
43944 MW
Background of TSG101
Seems to phosphorylate critical substrates that regulate the G1/S phase transition and/or DNA replication. Can phosphorylates MCM2 and MCM3.
Sato N., EMBO J. 16:4340-4351(1997).
Hess G.F., Gene 211:133-140(1998).
Jiang W., Proc. Natl. Acad. Sci. U.S.A. 94:14320-14325(1997).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:1,000-1:2,000
ICC: 1:100-1:500
IHC: 1:50-1:200
FC: 1:50-1:100
Validation Images & Assay Conditions
![a01233 1 anti tsg101 antibody icc testing 2 a01233 1 anti tsg101 antibody icc testing 2](https://www.bosterbio.com/media/catalog/product/a/0/a01233-1-anti-tsg101-antibody-icc-testing-2.jpg)
Click image to see more details
Figure 2. Immunocytochemistry staining of TSG101 using Anti-TSG101 Antibody (A01233-1).
ICC staining TSG101 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde![a01233 1 anti tsg101 antibody wb testing 1 a01233 1 anti tsg101 antibody wb testing 1](https://www.bosterbio.com/media/catalog/product/a/0/a01233-1-anti-tsg101-antibody-wb-testing-1.jpg)
Click image to see more details
Figure 1. Western blotting validation for Anti-TSG101 Antibody A01233-1
Western blot analysis of TSG101 on different lysates using anti-TSG101 antibody at 1/1Protein Target Info & Infographic
Gene/Protein Information For TSG101 (Source: Uniprot.org, NCBI)
Gene Name
TSG101
Full Name
Tumor susceptibility gene 101 protein
Weight
43944 MW
Superfamily
ubiquitin-conjugating enzyme family
Alternative Names
ESCRT-I complex subunit TSG101; TSG10; tumor susceptibility gene 10; tumor susceptibility gene 101 protein; tumor susceptibility gene 101; tumor susceptibility protein; VPS23 TSG101 TSG10, VPS23 tumor susceptibility 101 tumor susceptibility gene 101 protein|ESCRT-I complex subunit TSG101|tumor susceptibility gene 10|tumor susceptibility gene 101|tumor susceptibility protein
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on TSG101, check out the TSG101 Infographic
![TSG101 infographic](/media/images/gene-infographic-example.jpg)
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for TSG101: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-TSG101 Antibody (A01233-1)
Hello CJ!
A01233-1 has been cited in 1 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Pei-pei Fang,Chen-wei Pan,Wei Lin,Jie Li,Shan-shan Huang,Guang-yao Zhou,Wen-jun Du,Qiang Li, "ASK1 Enhances Angiotensin II-Induced Liver Fibrosis In Vitro by Mediating Endoplasmic Reticulum Stress-Dependent Exosomes",Mediators of Inflammation,vol.2020,Art
Species: Human
A01233-1 usage in article: APP:WB, SAMPLE:LX-2 CELLS, DILUTION:1:1000
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