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Product Info Summary
| SKU: | PB9271 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-TRPC5 Antibody Picoband®
SKU/Catalog Number
PB9271
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-TRPC5 Antibody Picoband® catalog # PB9271. Tested in WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-TRPC5 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9271)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human TRPC5 recombinant protein (Position: K684-L973). Human TRPC5 shares 90% amino acid (aa) sequence identity with mouse TRPC5.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PB9271 is reactive to TRPC5 in Human
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
111 kDa
Calculated molecular weight
111412 MW
Background of TRPC5
Short transient receptor potential channel 5 (TrpC5), also known as TRP-5, is a protein that in humans is encoded by the TRPC5 gene. TRPC5 is subtype of the TRPC family of mammalian transient receptor potential ion channels. It is mapped to Xq23. The predicted 973-amino acid TRPC5 protein has a calculated molecular mass of 111.5 kD. It contains the characteristic 8 predicted transmembrane domains (TM1 through TM8), including a pore region (TM7) between TM6 and TM8. TRPC5 is a multi-pass membrane protein and is thought to form a receptor-activated non-selective calcium permeant cation channel, and it is a candidate for the regulation of calcium waves. The protein is active alone or as a heteromultimeric assembly with TRPC1, TRPC3, and TRPC4.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PB9271 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human
Positive Control
WB: HELA Whole Cell, U87 Whole Cell, COLO320 Whole Cell
Validation Images & Assay Conditions
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Anti-TRPC5 Picoband antibody, PB9271, Western blotting
All lanes: Anti TRPC5 (PB9271) at 0.5ug/ml
WB: Recombinant Human TRPC5 Protein 0.5ng
Predicted bind size: 50KD
Observed bind size: 50KD
Click image to see more details
Anti-TRPC5 Picoband antibody, PB9271, Western blotting
All lanes: Anti TRPC5 (PB9271) at 0.5ug/ml
Lane 1: HELA Whole Cell Lysate at 40ug
Lane 2: U87 Whole Cell Lysate at 40ug
Lane 3: COLO320 Whole Cell Lysate at 40ug
Predicted bind size: 111KD
Observed bind size: 111KD
Click image to see more details
Figure 3. Western blot analysis of TRPC5 using anti-TRPC5 antibody (PB9271).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TRPC5 antibody (PB9271) at 1:200 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:1000 for 1.5 hour at RT. The signal is developed using a fluorescent. A specific band was detected for TRPC5 at approximately 111 kDa. The expected band size for TRPC5 is at 111 kDa.
Protein Target Info & Infographic
Gene/Protein Information For TRPC5 (Source: Uniprot.org, NCBI)
Gene Name
TRPC5
Full Name
Short transient receptor potential channel 5
Weight
111412 MW
Superfamily
transient receptor (TC 1.A.4) family
Alternative Names
Short transient receptor potential channel 5;TrpC5;Transient receptor protein 5;TRP-5;hTRP-5;hTRP5;TRPC5;TRP5; TRPC5 PPP1R159, TRP5 transient receptor potential cation channel subfamily C member 5 short transient receptor potential channel 5|TRP-5|hTRP5|protein phosphatase 1, regulatory subunit 159|transient receptor potential channel 5|transient receptor protein 5
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on TRPC5, check out the TRPC5 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for TRPC5: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-TRPC5 Antibody Picoband® (PB9271)
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Customer Reviews
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1 Reviews For Anti-TRPC5 Antibody Picoband®
0
Anti-TRPC5 Tried in Human Tissue Sample--Saikat Chakraborty, Psychiatry / Neuroscience, Northwestern University Feinberg School of Medicine, Research Scientist
Excellent
Source: Biocompare.com
| Application | Western Blot |
|---|---|
| Sample | Human Tissue |
| Detection | Fluorescent based |
"We are comparing the expression of the TRPC5 protein in psychological symptoms characterized in a human tissue lysate. This Anti-TRPC5 antibody worked perfectly in detection in human tissue. Perfect band obtained at desired molecular weight. No extra bands. I recommend."
Saikat Chakraborty
Verified customer
Submitted 2019-02-15
Customer Q&As
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4 Customer Q&As for Anti-TRPC5 Antibody Picoband®
Question
See below the WB image, lot number and protocol we used for frontal cortex using anti-TRPC5 antibody PB9271. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2019-12-23
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-12-23
Question
We are currently using anti-TRPC5 antibody PB9271 for human tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says human. Is it likely that the antibody can work on pig tissues as well?
Verified Customer
Verified customer
Asked: 2019-11-04
Answer
The anti-TRPC5 antibody (PB9271) has not been validated for cross reactivity specifically with pig tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in pig you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-11-04
Question
Does PB9271 anti-TRPC5 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2018-12-26
Answer
You can see on the product datasheet, PB9271 anti-TRPC5 antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2018-12-26
Question
Will anti-TRPC5 antibody PB9271 work on monkey WB with fetal brain?
K. Taylor
Verified customer
Asked: 2017-05-25
Answer
Our lab technicians have not tested anti-TRPC5 antibody PB9271 on monkey. You can run a BLAST between monkey and the immunogen sequence of anti-TRPC5 antibody PB9271 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated monkey samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in monkey fetal brain in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2017-05-25
