Anti-TRNAU1AP Antibody Picoband™

Product Name

Anti-TRNAU1AP Antibody Picoband™

View all TRSPAP1 Antibodies

SKU/Catalog Number

A15424-1

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-TRNAU1AP Antibody Picoband™ catalog # A15424-1. Tested in ELISA, WB applications. This antibody reacts with Human.

Storage & Handling

At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Cite This Product

Anti-TRNAU1AP Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A15424-1)

Host

Rabbit

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E.coli-derived human TRNAU1AP recombinant protein (Position: R21-E282).

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins.

Reactive Species

A15424-1 is reactive to TRNAU1AP in Human

Applications

A15424-1 is guaranteed for ELISA, WB Boster Guarantee

Observed Molecular Weight

40 kDa

Calculated molecular weight

24145 MW

Background of TRSPAP1

Enables RNA binding activity. Predicted to be involved in selenocysteine incorporation. Predicted to be located in cytoplasm. Predicted to be active in nucleus. TRNAU1AP binds selenocysteine tRNA (tRNA-sec; see 165060) and is a component of the selenocysteine biosynthesis pathway.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconstitution

Adding 0.2 ml of distilled water will yield a concentration of 500 µg/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.25-0.5 µg/ml, Human
Direct ELISA, 0.1-0.5 µg/ml, Human

Validation Images & Assay Conditions

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Figure 1. Western blot analysis of TRNAU1AP using anti-TRNAU1AP antibody (A15424-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human 293T whole cell lysates,
Lane 2: human Jurkat whole cell lysates,
Lane 3: human A549 whole cell lysates,
Lane 4: human Hela whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TRNAU1AP antigen affinity purified polyclonal antibody (Catalog # A15424-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TRNAU1AP at approximately 40 kDa. The expected band size for TRNAU1AP is at 32 kDa.

Gene/Protein Information For TRNAU1AP (Source: Uniprot.org, NCBI)

Alternative Names

FLJ20503; PRO1902; SECp43; SECP43RP4-669K10.4; tRNA selenocysteine 1 associated protein 1; tRNA selenocysteine 1-associated protein 1; tRNA selenocysteine associated protein (SECP43); tRNA selenocysteine associated protein 1; tRNA selenocysteine-associated protein 1; TRSPAP1 TRNAU1AP PRO1902, SECP43, TRSPAP1 tRNA selenocysteine 1 associated protein 1 tRNA selenocysteine 1-associated protein 1|tRNA selenocysteine associated protein (SECP43)|tRNA selenocysteine-associated protein 1

For more info on TRNAU1AP, check out the TRNAU1AP Infographic

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In this infographic, you will see the following information for TRNAU1AP: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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