Anti-TrkCT1 NTRK3 Antibody

Product Name

Anti-TrkCT1 NTRK3 Antibody

View all TrkC Antibodies

SKU/Catalog Number

A02502

Size

100ug

Form

Liquid (sterile filtered)

Description

Boster Bio Anti-TrkCT1 NTRK3 Antibody (Catalog # A02502). Tested in ELISA, IP, WB applications. This antibody reacts with Human, Mouse.

Storage & Handling

Store vial at -20°C prior to opening. Aliquot contents and freeze at -20°C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening. (Ship on dry ice.)

Cite This Product

Anti-TrkCT1 NTRK3 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A02502)

Host

Rabbit

Contents

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 0.01% (w/v) Sodium Azide

Clonality

Polyclonal

Isotype

IgG

Immunogen

This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to amino acids near the carboxyl terminus of mouse TrkCT1 protein.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

A02502 is reactive to NTRK3 in Human, Mouse

Applications

A02502 is guaranteed for ELISA, IP, WB Boster Guarantee

Observed Molecular Weight

42 kDa

Calculated molecular weight

94428 MW

Background of TrkC

This antibody is suitable for Cancer, Immunology and Nuclear Signaling research. TrkCT1, also named neurotrophic tyrosine kinase receptor type 3 (Ntrk3) non-catalytic isoform 2, is a non-catalytic isoform of TrkC, the high affinity receptor for Neurotrophin-3 (NT-3). This isoform lacks the kinase domain that is responsible for signaling by the full-length isoform.  TrkCT1 is the product of an alternative splicing of Ntrk3 that leaves the extracellular and transmembrane domains intact but includes a shorter intracellular domain encoded by exons 13b and 14b. Recent studies indicate that the short cytoplasmic tail binds the scaffold protein Tamalin in a ligand-dependent manner and further activates the Arf6–Rac1 signaling pathway. Isoform 3 transcripts are readily detected early during embryogenesis and are expressed predominantly in adult brain and gonads.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

ELISA: 1:5,000 - 1:20,000
IP: 1 μg
WB: 1:1,000 - 1:10,000

Validation Images & Assay Conditions

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Western blot analysis of TrkCT1 expression in non-transfected control (lane 1) and HEK293 cells (Lane 2). TrkCT1 was detected using rabbit anti-TrkCT1 Antigen Affinity purified polyclonal antibody (Catalog # A02502) at 1:3000. Personal Communication, V. Coppola, CCR-NCI, Frederick, MD.

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Western blot analysis of TrkCT1 expression in mouse cortex lysate (lane 1) and TrkCT1 knock-out cortex lysate (Lane 2). TrkCT1 was detected using rabbit anti-TrkCT1 Antigen Affinity purified polyclonal antibody (Catalog # A02502) at 1:6000. Personal Communication, V. Coppola, CCRNCI, Frederick, MD.

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Mouse cortex lysate was immunoprecipitated with anti-TrkCT1 antibody and further blotted with affinity purified anti-TrkCT1. Lane 1 is wild-type cortex lysate, Lane 2 is Tamalin knock-out cortex lysate, and Lane 3 is TrkCT1 knock-out cortex lysate. The membrane was probed with the primary antibody at a 1:6000 dilution. Personal Communication, V. Coppola, CCR-NCI, Frederick, MD.

Gene/Protein Information For NTRK3 (Source: Uniprot.org, NCBI)

Alternative Names

EC 2.7.10; EC 2.7.10.1; ETS related protein-neurotrophic receptor tyrosine kinase fusion protein; ETV6-NTRK3 fusion; gp145(trkC); GP145-TrkC; Neurotrophic tyrosine kinase receptor type 3; neurotrophic tyrosine kinase, receptor, type 3; NT 3 receptor; NT-3 growth factor receptor; NTRK3; TrkC tyrosine kinase; TrkC; trk-C; TRKCneurotrophin 3 receptor; tyrosine kinase receptor C NTRK3 GP145-TrkC, TRKC, gp145(trkC) neurotrophic receptor tyrosine kinase 3 NT-3 growth factor receptor|ETS related protein-neurotrophic receptor tyrosine kinase fusion protein|ETV6-NTRK3 fusion|neurotrophic tyrosine kinase, receptor, type 3|tyrosine kinase receptor C

For more info on NTRK3, check out the NTRK3 Infographic

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In this infographic, you will see the following information for NTRK3: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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