Product Info Summary
SKU: | A06668-2 |
---|---|
Size: | 100 μg/vial |
Reactive Species: | Human, Mouse, Rat |
Host: | Rabbit |
Application: | ELISA, Flow Cytometry, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-Tripeptidyl peptidase II/TPPII/TPP2 Antibody Picoband®
View all Tripeptidyl peptidase II Antibodies
SKU/Catalog Number
A06668-2
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Tripeptidyl peptidase II/TPPII/TPP2 Antibody Picoband® catalog # A06668-2. Tested in ELISA, Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-Tripeptidyl peptidase II/TPPII/TPP2 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A06668-2)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human Tripeptidyl peptidase II/TPPII/TPP2 recombinant protein (Position: E32-K1001).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A06668-2 is reactive to TPP2 in Human, Mouse, Rat
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500 µg/ml.
Observed Molecular Weight
138 kDa
Calculated molecular weight
104553 MW
Background of Tripeptidyl peptidase II
Tripeptidyl-peptidase 2 is an enzyme that in humans is encoded by the TPP2 gene. This gene encodes a mammalian peptidase that, at neutral pH, removes tripeptides from the N terminus of longer peptides. The protein has a specialized function that is essential for some MHC class I antigen presentation. The protein is a high molecular mass serine exopeptidase; the amino acid sequence surrounding the serine residue at the active site is similar to the peptidases of the subtilisin class rather than the trypsin class.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A06668-2 is guaranteed for ELISA, Flow Cytometry, IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.25 µg/ml, Human, Mouse, Rat
Immunohistochemistry(Paraffin-embedded Section), 2-5 µg/ml, Human
Immunocytochemistry/Immunofluorescence, 5 µg/ml, Human
Flow Cytometry (Fixed), 1-3 µg/1x106 cells, Human
Direct ELISA, 0.1-0.5 µg/ml, Human
Positive Control
WB: human Jurkat whole cell, human Hela whole cell, human HepG2 whole cell, human HEL whole cell, human K562 whole cell, rat liver tissue, rat testis tissue, rat brain tissue, rat RH35 whole cell, mouse liver tissue, mouse testis tissue, mouse brain tissue, mouse NIH/3T3 whole cell
IHC: human colorectal adenocarcinoma tissue, human liver cancer tissue, human lung cancer tissue
ICC/IF: A549 cell
FCM: SiHa cell
Validation Images & Assay Conditions
![a06668 2 tpp2 primary antibodies wb testing 1 a06668 2 tpp2 primary antibodies wb testing 1](https://www.bosterbio.com/media/catalog/product/a/0/a06668-2-tpp2-primary-antibodies-wb-testing-1.jpg)
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Figure 1. Western blot analysis of Tripeptidyl peptidase II/TPPII/TPP2 using anti-Tripeptidyl peptidase II/TPPII/TPP2 antibody (A06668-2).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Jurkat whole cell lysates,
Lane 2: human Hela whole cell lysates,
Lane 3: human HepG2 whole cell lysates,
Lane 4: human HEL whole cell lysates,
Lane 5: human K562 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Tripeptidyl peptidase II/TPPII/TPP2 antigen affinity purified polyclonal antibody (Catalog # A06668-2) at 0.25 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Tripeptidyl peptidase II/TPPII/TPP2 at approximately 138 kDa. The expected band size for Tripeptidyl peptidase II/TPPII/TPP2 is at 138 kDa.
![a06668 2 tpp2 primary antibodies wb testing 2 a06668 2 tpp2 primary antibodies wb testing 2](https://www.bosterbio.com/media/catalog/product/a/0/a06668-2-tpp2-primary-antibodies-wb-testing-2.jpg)
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Figure 2. Western blot analysis of Tripeptidyl peptidase II/TPPII/TPP2 using anti-Tripeptidyl peptidase II/TPPII/TPP2 antibody (A06668-2).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: rat liver tissue lysates,
Lane 2: rat testis tissue lysates,
Lane 3: rat brain tissue lysates,
Lane 4: rat RH35 whole cell lysates,
Lane 5: mouse liver tissue lysates,
Lane 6: mouse testis tissue lysates,
Lane 7: mouse brain tissue lysates,
Lane 8: mouse NIH/3T3 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Tripeptidyl peptidase II/TPPII/TPP2 antigen affinity purified polyclonal antibody (Catalog # A06668-2) at 0.25 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Tripeptidyl peptidase II/TPPII/TPP2 at approximately 138 kDa. The expected band size for Tripeptidyl peptidase II/TPPII/TPP2 is at 138 kDa.
![a06668 2 tpp2 primary antibodies ihc testing 3 a06668 2 tpp2 primary antibodies ihc testing 3](https://www.bosterbio.com/media/catalog/product/a/0/a06668-2-tpp2-primary-antibodies-ihc-testing-3.jpg)
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Figure 3. IHC analysis of Tripeptidyl peptidase II/TPPII/TPP2 using anti-Tripeptidyl peptidase II/TPPII/TPP2 antibody (A06668-2).
Tripeptidyl peptidase II/TPPII/TPP2 was detected in a paraffin-embedded section of human colorectal adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Tripeptidyl peptidase II/TPPII/TPP2 Antibody (A06668-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
![a06668 2 tpp2 primary antibodies ihc testing 4 a06668 2 tpp2 primary antibodies ihc testing 4](https://www.bosterbio.com/media/catalog/product/a/0/a06668-2-tpp2-primary-antibodies-ihc-testing-4.jpg)
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Figure 4. IHC analysis of Tripeptidyl peptidase II/TPPII/TPP2 using anti-Tripeptidyl peptidase II/TPPII/TPP2 antibody (A06668-2).
Tripeptidyl peptidase II/TPPII/TPP2 was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Tripeptidyl peptidase II/TPPII/TPP2 Antibody (A06668-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
![a06668 2 tpp2 primary antibodies ihc testing 5 a06668 2 tpp2 primary antibodies ihc testing 5](https://www.bosterbio.com/media/catalog/product/a/0/a06668-2-tpp2-primary-antibodies-ihc-testing-5.jpg)
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Figure 5. IHC analysis of Tripeptidyl peptidase II/TPPII/TPP2 using anti-Tripeptidyl peptidase II/TPPII/TPP2 antibody (A06668-2).
Tripeptidyl peptidase II/TPPII/TPP2 was detected in a paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Tripeptidyl peptidase II/TPPII/TPP2 Antibody (A06668-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
![a06668 2 tpp2 primary antibodies if testing 6 a06668 2 tpp2 primary antibodies if testing 6](https://www.bosterbio.com/media/catalog/product/a/0/a06668-2-tpp2-primary-antibodies-if-testing-6.jpg)
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Figure 6. IF analysis of Tripeptidyl peptidase II/TPPII/TPP2 using anti-Tripeptidyl peptidase II/TPPII/TPP2 antibody (A06668-2).
Tripeptidyl peptidase II/TPPII/TPP2 was detected in an immunocytochemical section of A549 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-Tripeptidyl peptidase II/TPPII/TPP2 Antibody (A06668-2) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
![a06668 2 tpp2 primary antibodies fcm testing 7 a06668 2 tpp2 primary antibodies fcm testing 7](https://www.bosterbio.com/media/catalog/product/a/0/a06668-2-tpp2-primary-antibodies-fcm-testing-7.jpg)
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Figure 7. Flow Cytometry analysis of SiHa cells using anti-Tropomyosin 2/TPM2 antibody (A06668-2).
Overlay histogram showing SiHa cells stained with A06668-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Tropomyosin 2/TPM2 Antibody (A06668-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Protein Target Info & Infographic
Gene/Protein Information For TPP2 (Source: Uniprot.org, NCBI)
Gene Name
TPP2
Full Name
Tripeptidyl-peptidase 2
Weight
104553 MW
Superfamily
peptidase S8 family
Alternative Names
Ubiquitin carboxyl-terminal hydrolase 21; Deubiquitinating enzyme 21; Ubiquitin thioesterase 21; Ubiquitin-specific-processing protease 21; USP21; USP23; PP1490 TPP2 IMD78, TPP-2, TPP-II, TPPII tripeptidyl peptidase 2 tripeptidyl-peptidase 2|tripeptidyl aminopeptidase|tripeptidyl-peptidase II
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on TPP2, check out the TPP2 Infographic
![TPP2 infographic](/media/images/gene-infographic-example.jpg)
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for TPP2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Tripeptidyl peptidase II/TPPII/TPP2 Antibody Picoband® (A06668-2)
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