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Product Info Summary
| SKU: | A05449 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC-P, ICC, WB |
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Product info
Product Name
Anti-TRIP12 Antibody
View all Thyroid receptor-interacting protein 12 Antibodies
SKU/Catalog Number
A05449
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-TRIP12 Antibody (Catalog # A05449). Tested in ELISA, WB, IHC-P, IF, ICC applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
TRIP12 antibody can be stored at 4°C for three months and -20°C, stable for up to one year.
Cite This Product
Anti-TRIP12 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A05449)
Host
Rabbit
Contents
TRIP12 antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
TRIP12 antibody was raised against a 18 amino acid peptide near the carboxy terminus of human TRIP12. The immunogen is located within amino acids 1930 - 1980 of TRIP12.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
TRIP12 antibody is human, mouse and rat reactive. At least four isoforms are known to exist.
Reactive Species
A05449 is reactive to TRIP12 in Human, Mouse, Rat
Applications
A05449 is guaranteed for ELISA, IF, IHC-P, ICC, WB Boster Guarantee
Observed Molecular Weight
68 kDa
Calculated molecular weight
220434 MW
Background of Thyroid receptor-interacting protein 12
Thyroid hormone receptors (TRs) are transcription factors that regulate the expression of specific genes in a hormone-dependent manner (1). TRIP12 (thyroid hormone receptor interactor 12) is an ATP-dependent E3 ubiquitin ligase involved in the human ubiquitin fusion degradation (UFD) pathway and also modulates the NEDD8 pathway (2,3). TRIP12 contains one WWE domain and a single HECT (E6AP-type E3 ubiquitin-protein ligase) domain suggested to contain a noncovalent ubiquitin-binding site (4). TRIP12 acts as a key regulator of DNA damage response and the ubiquitin ligase activity of TRIP12 is essential for mouse development (5).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
TRIP12 antibody can be used for detection of TRIP12 by Western blot at 1 - 2 μg/ml. Antibody can also be used for Immunohistochemistry starting at 5 μg/mL. For immunocytochemistry start at 2 μg/mL.For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in rat samples; Immunohistochemistry in rat samples; Immunocytochemistry in human samples; and Immunofluorescence in rat samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Western blot analysis of TRIP12 in rat colon tissue lysate with TRIP12 antibody at 1 μg/ml.
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Immunohistochemistry of TRIP12 in rat colon tissue with TRIP12 antibody at 5 μg/ml.
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Immunofluorescence of TRIP12 in rat colon tissue with TRIP12 antibody at 20 μg/ml.
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Immunocytochemistry of TRIP12 in Hela cells with TRIP12 Antibody at 2 μg/ml.
Protein Target Info & Infographic
Gene/Protein Information For TRIP12 (Source: Uniprot.org, NCBI)
Gene Name
TRIP12
Full Name
E3 ubiquitin-protein ligase TRIP12
Weight
220434 MW
Superfamily
UPL family
Alternative Names
EC 6.3.2.-; KIAA0045ULF; MGC138849; MGC138850; probable E3 ubiquitin-protein ligase TRIP12; thyroid hormone receptor interactor 12; thyroid receptor interacting protein 12; Thyroid receptor-interacting protein 12; TR-interacting protein 12; TRIP-12 TRIP12 MRD49, TRIP-12, TRIPC, ULF thyroid hormone receptor interactor 12 E3 ubiquitin-protein ligase TRIP12|E3 ubiquitin-protein ligase for Arf|HECT-type E3 ubiquitin transferase TRIP12|TR-interacting protein 12|probable E3 ubiquitin-protein ligase TRIP12|thyroid receptor interacting protein 12
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on TRIP12, check out the TRIP12 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for TRIP12: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-TRIP12 Antibody (A05449)
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