Click image to see more details
-
-
-
-
-
+2
Product Info Summary
| SKU: | PA2010 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IHC, IHC-F, ICC, WB |
Customers Who Bought This Also Bought
Product info
Product Name
Anti-TRAM1 Antibody Picoband®
SKU/Catalog Number
PA2010
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-TRAM1 Antibody catalog # PA2010. Tested in IHC, IHC-F, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-TRAM1 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA2010)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of human TRAM1, identical to the related rat and mouse sequences.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PA2010 is reactive to TRAM1 in Human, Mouse, Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
56 kDa
Calculated molecular weight
43072 MW
Background of TRAM1
TRAM1 (Translocation-Associating Membrane Protein 1), By crosslinking and reconstitution of canine proteoliposomes, followed by microsequencing and PCR screening of canine kidney and HeLa cell cDNA libraries, Gorlich et al. (1992) isolated cDNAs encoding TRAM (translocating chain-associating membrane protein). The International Radiation Hybrid Mapping Consortium mapped the TRAM gene to chromosome 8. Sequence analysis predicted that human TRAM is a 374-amino acid, 8-pass transmembrane protein that shares 95% amino acid identity with the canine protein. Functional analysis indicated that TRAM influences glycosylation and is stimulatory or required for the translocation of secretory proteins.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PA2010 is guaranteed for IHC, IHC-F, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunocytochemistry , 0.5-1μg/ml, Human, Mouse, Rat
Immunohistochemistry (Frozen Section), 0.5-1μg/ml, Rat, Human, Mouse
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Rat, Mouse, By Heat
Western blot, 0.1-0.5μg/ml, Human, Rat, Mouse
Positive Control
WB: Rat Brain Tissue, Rat Kidney Tissue, 293T Cell, RAJI Cell, JURKAT Cell, Hela Cell,
IHC: Human Lung Cancer Tissue, Rat Kidney Tissue
IHC-F: rat intestine tissue, rat brain tissue
ICC: HELA Cell
Validation Images & Assay Conditions
Click image to see more details
Anti-TRAM1 antibody, PA2010, IHC(P)
IHC(P): Human Lung Cancer Tissue
Click image to see more details
Anti-TRAM1 antibody, PA2010, IHC(P)
IHC(P): Rat Kidney Tissue
Click image to see more details
Anti-TRAM1 antibody, PA2010, ICC
ICC: HELA Cell
Click image to see more details
Anti-TRAM1 antibody, PA2010,WB,
Lane 1: Rat Brain Tissue Lysate
Lane 2: Rat Kidney Tissue Lysate
Lane 3: 293T Cell Lysate
Lane 4: RAJI Cell Lysate
Lane 5: JURKAT Cell Lysate
Lane 6: Hela Cell Lysate
Click image to see more details
Figure 5. IHC analysis of TRAM1 using anti-TRAM1 antibody (PA2010).
TRAM1 was detected in frozen section of rat intestine tissues. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-TRAM1 Antibody (PA2010) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Click image to see more details
Figure 6. IHC analysis of TRAM1 using anti-TRAM1 antibody (PA2010).
TRAM1 was detected in frozen section of rat brain tissues. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-TRAM1 Antibody (PA2010) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Protein Target Info & Infographic
Gene/Protein Information For TRAM1 (Source: Uniprot.org, NCBI)
Gene Name
TRAM1
Full Name
Translocating chain-associated membrane protein 1
Weight
43072 MW
Superfamily
TRAM family
Alternative Names
Translocating chain-associated membrane protein 1;TRAM1;TRAM; TRAM1 PNAS8, TRAM, TRAMP translocation associated membrane protein 1 translocating chain-associated membrane protein 1|translocating chain-associating membrane protein|translocation-associating membrane protein 1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on TRAM1, check out the TRAM1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for TRAM1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-TRAM1 Antibody Picoband® (PA2010)
Hello CJ!
No publications found for PA2010
*Do you have publications using this product? Share with us and receive a reward. Ask us for more details.
Recommended Resources
Here are featured tools and databases that you might find useful.
- Boster's Pathways Library
- Protein Databases
- Bioscience Research Protocol Resources
- Data Processing & Analysis Software
- Photo Editing Software
- Scientific Literature Resources
- Research Paper Management Tools
- Molecular Biology Software
- Primer Design Tools
- Bioinformatics Tools
- Phylogenetic Tree Analysis
Customer Reviews
Have you used Anti-TRAM1 Antibody Picoband®?
Submit a review and receive an Amazon gift card.
- $30 for a review with an image
0 Reviews For Anti-TRAM1 Antibody Picoband®
Customer Q&As
Have a question?
Find answers in Q&As, reviews.
Can't find your answer?
Submit your question
6 Customer Q&As for Anti-TRAM1 Antibody Picoband®
Question
Will anti-TRAM1 antibody PA2010 work for IHC-P with choroid plexus epithelium?
Verified Customer
Verified customer
Asked: 2019-11-13
Answer
According to the expression profile of choroid plexus epithelium, TRAM1 is highly expressed in choroid plexus epithelium. So, it is likely that anti-TRAM1 antibody PA2010 will work for IHC-P with choroid plexus epithelium.
Boster Scientific Support
Answered: 2019-11-13
Question
We are currently using anti-TRAM1 antibody PA2010 for mouse tissue, and we are well pleased with the ICC results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on horse tissues as well?
Verified Customer
Verified customer
Asked: 2019-08-15
Answer
The anti-TRAM1 antibody (PA2010) has not been validated for cross reactivity specifically with horse tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in horse you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-08-15
Question
I was wanting to use your anti-TRAM1 antibody for IHC-P for mouse choroid plexus epithelium on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for mouse choroid plexus epithelium identification?
Verified Customer
Verified customer
Asked: 2019-05-30
Answer
You can see on the product datasheet, PA2010 anti-TRAM1 antibody has been tested for IHC-P, IHC-F, ICC, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in mouse choroid plexus epithelium in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-05-30
Question
Is this PA2010 anti-TRAM1 antibody reactive to the isotypes of TRAM1?
Verified Customer
Verified customer
Asked: 2019-01-21
Answer
The immunogen of PA2010 anti-TRAM1 antibody is A synthetic peptide corresponding to a sequence at the C-terminus of human TRAM1(314-331aa KFINFQLRRWREHSAFQA), identical to the related rat and mouse sequences. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-01-21
Question
Can you help my question with product PA2010, anti-TRAM1 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2018-07-19
Answer
We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free PA2010 anti-TRAM1 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2018-07-19
Question
Thank you for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for choroid plexus epithelium using anti-TRAM1 antibody PA2010. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2017-09-29
Answer
Thank you for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2017-09-29
