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Product Info Summary
| SKU: | M00185-1 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | Flow Cytometry, IHC, WB |
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Product info
Product Name
Anti-TRAF6 Rabbit Monoclonal Antibody
SKU/Catalog Number
M00185-1
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-TRAF6 Rabbit Monoclonal Antibody catalog # M00185-1. Tested in WB, IHC, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-TRAF6 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00185-1)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
BOF-20
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human TRAF6
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M00185-1 is reactive to TRAF6 in Human, Mouse, Rat
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
70 kDa
Calculated molecular weight
59573 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00185-1 is guaranteed for Flow Cytometry, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:50-1:200
FC 1:50-1:200
Positive Control
IHC: Rat ovary tissue, Rat stomach tissue, Human prostate cancer tissue, Human astrocytoma tissue, Mouse skeletal muscle - gastrocnemius tissue, human colon carcinoma tissue
Validation Images & Assay Conditions
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All lanes use the Antibody at 1:3K dilution for 1 hour at room temperature.
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All lanes use the Antibody at 1:3K dilution for 1 hour at room temperature.
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All lanes use the Antibody at 1:3K dilution for 1 hour at room temperature.
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All lanes use the Antibody at 1:3K dilution for 1 hour at room temperature.
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Western blot analysis of TRAF6 expression in NIH/3T3 cell lysate.
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Immunohistochemical analysis of paraffin-embedded Rat ovary, using the Antibody at 1:50 dilution.
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Immunohistochemical analysis of paraffin-embedded Rat stomach, using the Antibody at 1:50 dilution.
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Immunohistochemical analysis of paraffin-embedded Human prostate cancer, using the Antibody at 1:50 dilution.
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Immunohistochemical analysis of paraffin-embedded Human astrocytoma, using the Antibody at 1:50 dilution.
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Immunohistochemical analysis of paraffin-embedded Mouse skeletal muscle - gastrocnemius , using the Antibody at 1:50 dilution.
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Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using TRAF6 Antibody.
Protein Target Info & Infographic
Gene/Protein Information For TRAF6 (Source: Uniprot.org, NCBI)
Gene Name
TRAF6
Full Name
TNF receptor-associated factor 6
Weight
59573 MW
Superfamily
TNF receptor-associated factor family
Alternative Names
TNF receptor-associated factor 6;6.3.2.-;E3 ubiquitin-protein ligase TRAF6;Interleukin-1 signal transducer;RING finger protein 85;TRAF6;RNF85; TRAF6 MGC:3310, RNF85 TNF receptor associated factor 6 TNF receptor-associated factor 6|E3 ubiquitin-protein ligase TRAF6|RING finger protein 85|RING-type E3 ubiquitin transferase TRAF6|TNF receptor-associated factor 6, E3 ubiquitin protein ligase|interleukin-1 signal transducer
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on TRAF6, check out the TRAF6 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for TRAF6: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-TRAF6 Rabbit Monoclonal Antibody (M00185-1)
Hello CJ!
M00185-1 has been cited in 3 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Liu L,Liu F,Sun Z,Peng Z,You T,Yu Z. LncRNA NEAT1 promotes apoptosis and inflammation in LPS-induced sepsis models by targeting miR-590-3p. Exp Ther Med.2020 Oct; 20(4):3290-3300. doi:10.3892/etm.2020.9079. Epub 2020 Jul 29.PMID:32855700;PMCID:PMC7444425.
Species: Human
M00185-1 usage in article: APP:WB, SAMPLE:H9C2 CELL, DILUTION:1:500
Liu Mw, Wang Yh, Qian Cy, Li H. Int J Mol Med. 2014 Dec;34(6):1492-504. Doi: 10.3892/Ijmm.2014.1943. Epub 2014 Sep 23. Xuebijing Exerts Protective Effects On Lung Permeability Leakage And Lung Injury By Upregulating Toll-Interacting Protein Expres...
Yuan Z, Zhang J, Yang C. Evid Based Complement Alternat Med. 2014;2014:620359. Doi: 10.1155/2014/620359. Epub 2014 Aug 17. Ligusticum Wallichii Extract Inhibited The Expression Of Il-1?? After Ami In Rats.
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