Anti-TRAF5 Antibody Picoband®

TRAF-5 antibody

Boster Bio Anti-TRAF5 Antibody catalog # PA2154. Tested in WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Product Info Summary

SKU: PA2154
Size: 100 μg/vial
Reactive Species: Human
Host: Rabbit
Application: WB

Product Name

Anti-TRAF5 Antibody Picoband®

View all TRAF-5 Antibodies

SKU/Catalog Number

PA2154

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-TRAF5 Antibody catalog # PA2154. Tested in WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-TRAF5 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA2154)

Host

Rabbit

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2mg Na2HPO4, 0.05 mg NaN3.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

A synthetic peptide corresponding to a sequence at the N-terminus of human TRAF5, different from the related mouse sequence by two amino acids.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins

Reactive Species

PA2154 is reactive to TRAF5 in Human

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Observed Molecular Weight

18 kDa

Calculated molecular weight

64406 MW

Background of TRAF-5

TNF receptor-associated factor 5, also known as RNF84, is a protein that in humans is encoded by the TRAF5 gene. The scaffold protein encoded by this gene is a member of the tumor necrosis factor receptor-associated factor (TRAF) protein family. Fluorescence in situ hybridization confirmed the regional localization of human TRAF5 to chromosome 1q32.2-q32.3. This protein is one of the components of a multiple protein complex which binds to tumor necrosis factor (TNF) receptor cytoplasmic domains and mediates TNF-induced activation. It is an adapter protein and signal transducer that links members of the tumor necrosis factor receptor family to different signaling pathways by association with the receptor cytoplasmic domain and kinases. It seems to be involved in apoptosis.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

PA2154 is guaranteed for WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml, Human

Positive Control

WB: k562 Cell

Validation Images & Assay Conditions

Gene/Protein Information For TRAF5 (Source: Uniprot.org, NCBI)

Gene Name

TRAF5

Full Name

TNF receptor-associated factor 5

Weight

64406 MW

Superfamily

TNF receptor-associated factor family

Alternative Names

TNF receptor-associated factor 5;RING finger protein 84;TRAF5;RNF84; TRAF5 MGC:39780, RNF84 TNF receptor associated factor 5 TNF receptor-associated factor 5|RING finger protein 84

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on TRAF5, check out the TRAF5 Infographic

TRAF5 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for TRAF5: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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7 Customer Q&As for Anti-TRAF5 Antibody Picoband®

Question

We are currently using anti-TRAF5 antibody PA2154 for human tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human. Is it possible that the antibody can work on primate tissues as well?

Verified Customer

Verified customer

Asked: 2019-11-12

Answer

The anti-TRAF5 antibody (PA2154) has not been tested for cross reactivity specifically with primate tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in primate you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2019-11-12

Question

Please see the WB image, lot number and protocol we used for small intestine peyer's patch using anti-TRAF5 antibody PA2154. Please let me know if you require anything else.

Verified Customer

Verified customer

Asked: 2019-10-04

Answer

Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2019-10-04

Question

I see that the anti-TRAF5 antibody PA2154 works with WB, what is the protocol used to produce the result images on the product page?

Verified Customer

Verified customer

Asked: 2019-09-05

Answer

You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]

Boster Scientific Support

Answered: 2019-09-05

Question

Is there a BSA free version of anti-TRAF5 antibody PA2154 available?

Verified Customer

Verified customer

Asked: 2019-08-26

Answer

We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-TRAF5 antibody PA2154 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.

Boster Scientific Support

Answered: 2019-08-26

Question

Is this PA2154 anti-TRAF5 antibody reactive to the isotypes of TRAF5?

Verified Customer

Verified customer

Asked: 2018-09-17

Answer

The immunogen of PA2154 anti-TRAF5 antibody is A synthetic peptide corresponding to a sequence at the N-terminus of human TRAF5(62-75aa HRFCQHCILSLREL), different from the related mouse sequence by two amino acids. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?

Boster Scientific Support

Answered: 2018-09-17

Question

Is a blocking peptide available for product anti-TRAF5 antibody (PA2154)?

T. Dhar

Verified customer

Asked: 2017-07-27

Answer

We do provide the blocking peptide for product anti-TRAF5 antibody (PA2154). If you would like to place an order for it please contact [email protected] and make a special request.

Boster Scientific Support

Answered: 2017-07-27

Question

Will PA2154 anti-TRAF5 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

M. Singh

Verified customer

Asked: 2013-05-30

Answer

It shows on the product datasheet, PA2154 anti-TRAF5 antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2013-05-30

Order DetailsPrice
PA2154

100μg

$370
PA2154-10ug

10μg sample (liquid)

$99
PA2154-Biotin

100 μg Biotin conjugated

$570
PA2154-Cy3

100 μg Cy3 conjugated

$570
PA2154-Dylight488

100 μg Dylight488 conjugated

$570
PA2154-Dylight550

100 μg Dylight550 conjugated

$570
PA2154-Dylight594

100 μg Dylight594 conjugated

$570
PA2154-FITC

100 μg FITC conjugated

$570
PA2154-HRP

100 μg HRP conjugated

$570
PA2154-APC

100 μg APC conjugated

$670
PA2154-PE

100 μg PE conjugated

$670
PA2154-iFluor647

100 μg iFluor647 conjugated

$670
PA2154-carrier-free

Carrier Free

$370

More conjugates/formats

Free Secondary Antibody

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PA2154
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00

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