Product Info Summary
SKU: | PA2154 |
---|---|
Size: | 100 μg/vial |
Reactive Species: | Human |
Host: | Rabbit |
Application: | WB |
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Product info
Product Name
Anti-TRAF5 Antibody Picoband®
SKU/Catalog Number
PA2154
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-TRAF5 Antibody catalog # PA2154. Tested in WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-TRAF5 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA2154)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2mg Na2HPO4, 0.05 mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the N-terminus of human TRAF5, different from the related mouse sequence by two amino acids.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PA2154 is reactive to TRAF5 in Human
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
18 kDa
Calculated molecular weight
64406 MW
Background of TRAF-5
TNF receptor-associated factor 5, also known as RNF84, is a protein that in humans is encoded by the TRAF5 gene. The scaffold protein encoded by this gene is a member of the tumor necrosis factor receptor-associated factor (TRAF) protein family. Fluorescence in situ hybridization confirmed the regional localization of human TRAF5 to chromosome 1q32.2-q32.3. This protein is one of the components of a multiple protein complex which binds to tumor necrosis factor (TNF) receptor cytoplasmic domains and mediates TNF-induced activation. It is an adapter protein and signal transducer that links members of the tumor necrosis factor receptor family to different signaling pathways by association with the receptor cytoplasmic domain and kinases. It seems to be involved in apoptosis.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PA2154 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human
Positive Control
WB: k562 Cell
Validation Images & Assay Conditions
Click image to see more details
Anti-TRAF5 antibody, PA2154, Western blotting
WB: k562 Cell Lysate
Protein Target Info & Infographic
Gene/Protein Information For TRAF5 (Source: Uniprot.org, NCBI)
Gene Name
TRAF5
Full Name
TNF receptor-associated factor 5
Weight
64406 MW
Superfamily
TNF receptor-associated factor family
Alternative Names
TNF receptor-associated factor 5;RING finger protein 84;TRAF5;RNF84; TRAF5 MGC:39780, RNF84 TNF receptor associated factor 5 TNF receptor-associated factor 5|RING finger protein 84
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on TRAF5, check out the TRAF5 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for TRAF5: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-TRAF5 Antibody Picoband® (PA2154)
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No publications found for PA2154
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Customer Reviews
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Customer Q&As
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7 Customer Q&As for Anti-TRAF5 Antibody Picoband®
Question
We are currently using anti-TRAF5 antibody PA2154 for human tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human. Is it possible that the antibody can work on primate tissues as well?
Verified Customer
Verified customer
Asked: 2019-11-12
Answer
The anti-TRAF5 antibody (PA2154) has not been tested for cross reactivity specifically with primate tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in primate you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-11-12
Question
Please see the WB image, lot number and protocol we used for small intestine peyer's patch using anti-TRAF5 antibody PA2154. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2019-10-04
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-10-04
Question
I see that the anti-TRAF5 antibody PA2154 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-09-05
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2019-09-05
Question
Is there a BSA free version of anti-TRAF5 antibody PA2154 available?
Verified Customer
Verified customer
Asked: 2019-08-26
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-TRAF5 antibody PA2154 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-08-26
Question
Is this PA2154 anti-TRAF5 antibody reactive to the isotypes of TRAF5?
Verified Customer
Verified customer
Asked: 2018-09-17
Answer
The immunogen of PA2154 anti-TRAF5 antibody is A synthetic peptide corresponding to a sequence at the N-terminus of human TRAF5(62-75aa HRFCQHCILSLREL), different from the related mouse sequence by two amino acids. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2018-09-17
Question
Is a blocking peptide available for product anti-TRAF5 antibody (PA2154)?
T. Dhar
Verified customer
Asked: 2017-07-27
Answer
We do provide the blocking peptide for product anti-TRAF5 antibody (PA2154). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2017-07-27
Question
Will PA2154 anti-TRAF5 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
M. Singh
Verified customer
Asked: 2013-05-30
Answer
It shows on the product datasheet, PA2154 anti-TRAF5 antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2013-05-30