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Product Info Summary
| SKU: | A13599 |
|---|---|
| Size: | 100μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-GPR175 TPRA1 Antibody
SKU/Catalog Number
A13599
Size
100μl
Form
Liquid
Description
Boster Bio Anti-GPR175 TPRA1 Antibody catalog # A13599. Tested in WB applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-GPR175 TPRA1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A13599)
Host
Rabbit
Contents
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
The antiserum was produced against synthesized peptide derived from human GPR175. AA range:198-247
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A13599 is reactive to TPRA1 in Human, Mouse, Rat
Reconstitution
Calculated molecular weight
41053 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A13599 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB, 1:500-1:2000
Validation Images & Assay Conditions
Click image to see more details
Western Blot (WB) analysis of specific cells using GPR175 Polyclonal antibody.
Protein Target Info & Infographic
Gene/Protein Information For TPRA1 (Source: Uniprot.org, NCBI)
Gene Name
TPRA1
Full Name
Transmembrane protein adipocyte-associated 1
Weight
41053 MW
Superfamily
UPF0359 family
Alternative Names
Transmembrane protein adipocyte-associated 1;Integral membrane protein GPR175;Transmembrane protein 227;TPRA1;GPR175, TMEM227;PP6566;
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on TPRA1, check out the TPRA1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for TPRA1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-GPR175 TPRA1 Antibody (A13599)
Hello CJ!
A13599 has been cited in 2 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Alpinetin prevents inflammatory responses in OVA-induced allergic asthma through modulating PI3K/AKT/NF-κB and HO-1 signaling pathways in mice
Zhang Y,Han Z,Jiang A,Wu D,Li S,Liu Z,Wei Z,Yang Z,Guo C.Protective Effects of Pterostilbene on Lipopolysaccharide-Induced Acute Lung Injury in Mice by Inhibiting NF-κB and Activating Nrf2/HO-1 Signaling Pathways.Front Pharmacol.2021 Jan 29;11:591836.doi:
Species: Mouse
A13599 usage in article: APP:WB, SAMPLE:LUNG TISSUE, DILUTION:NA
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Customer Reviews
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Customer Q&As
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6 Customer Q&As for Anti-GPR175 TPRA1 Antibody
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for pancreas skin using anti-GPR175 antibody A13599. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2020-03-09
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2020-03-09
Question
Does anti-GPR175 antibody A13599 work on dog WB with fetal brain?
Verified Customer
Verified customer
Asked: 2019-07-22
Answer
Our lab technicians have not validated anti-GPR175 antibody A13599 on dog. You can run a BLAST between dog and the immunogen sequence of anti-GPR175 antibody A13599 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated dog samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in dog fetal brain in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-07-22
Question
I have a question about product A13599, anti-GPR175 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-06-14
Answer
We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A13599 anti-GPR175 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-06-14
Question
Is this A13599 anti-GPR175 antibody reactive to the isotypes of TPRA1?
Verified Customer
Verified customer
Asked: 2018-08-02
Answer
The immunogen of A13599 anti-GPR175 antibody is Synthesized peptide derived from the Internal region of human GPR175. at AA rangle: 170-250. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2018-08-02
Question
My question regards to test anti-GPR175 antibody A13599 on rat pancreas skin for research purposes, then I may be interested in using anti-GPR175 antibody A13599 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2017-10-20
Answer
The products we sell, including anti-GPR175 antibody A13599, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2017-10-20
Question
I see that the anti-GPR175 antibody A13599 works with WB, what is the protocol used to produce the result images on the product page?
E. Zhang
Verified customer
Asked: 2013-02-06
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2013-02-06
