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Product Info Summary
| SKU: | A07428 |
|---|---|
| Size: | 100μl |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | ELISA, WB |
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Product info
Product Name
Anti-Topo III alpha TOP3A Antibody
SKU/Catalog Number
A07428
Size
100μl
Form
Liquid
Description
Boster Bio Anti-Topo III alpha TOP3A Antibody catalog # A07428. Tested in ELISA, WB applications. This antibody reacts with Human, Mouse.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Topo III alpha TOP3A Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A07428)
Host
Rabbit
Contents
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthesized peptide derived from Topo IIIα . at AA range: 350-430
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A07428 is reactive to TOP3A in Human, Mouse
Reconstitution
Observed Molecular Weight
39 kDa
Calculated molecular weight
112372 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A07428 is guaranteed for ELISA, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB, 1:500-1:2000
ELISA 1:10000
Validation Images & Assay Conditions
Click image to see more details
Western blot analysis of 293T using Topo III Alpha antibody. Antibody was diluted at 1:500. Secondary antibody was diluted at 1:20000.
Protein Target Info & Infographic
Gene/Protein Information For TOP3A (Source: Uniprot.org, NCBI)
Gene Name
TOP3A
Full Name
DNA topoisomerase 3-alpha
Weight
112372 MW
Superfamily
type IA topoisomerase family
Alternative Names
DNA topoisomerase 3-alpha;5.99.1.2;DNA topoisomerase III alpha;TOP3A;TOP3; TOP3A MGRISCE2, PEOB5, TOP3, ZGRF7 DNA topoisomerase III alpha DNA topoisomerase 3-alpha|topo III-alpha|topoisomerase (DNA) III alpha|zinc finger, GRF-type containing 7
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on TOP3A, check out the TOP3A Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for TOP3A: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Topo III alpha TOP3A Antibody (A07428)
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No publications found for A07428
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Customer Reviews
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Customer Q&As
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7 Customer Q&As for Anti-Topo III alpha TOP3A Antibody
Question
I would like to test anti-Topo III alpha antibody A07428 on human thalamus trachea for research purposes, then I may be interested in using anti-Topo III alpha antibody A07428 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2019-06-20
Answer
The products we sell, including anti-Topo III alpha antibody A07428, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-06-20
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for thalamus trachea using anti-Topo III alpha antibody A07428. Let me know if you need anything else.
E. Mangal
Verified customer
Asked: 2017-09-13
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2017-09-13
Question
We are currently using anti-Topo III alpha antibody A07428 for mouse tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human, mouse. Is it true that the antibody can work on horse tissues as well?
Verified Customer
Verified customer
Asked: 2017-07-28
Answer
The anti-Topo III alpha antibody (A07428) has not been validated for cross reactivity specifically with horse tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in horse you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2017-07-28
Question
Will A07428 anti-Topo III alpha antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
B. Parker
Verified customer
Asked: 2016-08-09
Answer
It shows on the product datasheet, A07428 anti-Topo III alpha antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2016-08-09
Question
I was wanting to use your anti-Topo III alpha antibody for WB for human thalamus trachea on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human thalamus trachea identification?
N. Lewis
Verified customer
Asked: 2015-09-04
Answer
As indicated on the product datasheet, A07428 anti-Topo III alpha antibody has been tested for WB on human, mouse tissues. We have an innovator award program that if you test this antibody and show it works in human thalamus trachea in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2015-09-04
Question
Here is the WB image, lot number and protocol we used for thalamus trachea using anti-Topo III alpha antibody A07428. Please let me know if you require anything else.
E. Li
Verified customer
Asked: 2013-10-22
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2013-10-22
Question
My question regarding product A07428, anti-Topo III alpha antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
O. Bhatt
Verified customer
Asked: 2013-09-10
Answer
We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A07428 anti-Topo III alpha antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2013-09-10
