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Product Info Summary
| SKU: | M03166 |
|---|---|
| Size: | 50 µl |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | Flow Cytometry, IF, WB |
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Product info
Product Name
Anti-TOMM40 Antibody (N-Term)
SKU/Catalog Number
M03166
Size
50 µl
Description
Boster Bio Anti-TOMM40 Antibody (N-Term) (Catalog # M03166). Tested in WB, Flow Cytometry, IF application(s). This antibody reacts with Human.
Storage & Handling
Maintain refrigerated at 2-8°C for up to 2 weeks. For long-term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
Cite This Product
Anti-TOMM40 Antibody (N-Term) (Boster Biological Technology, Pleasanton CA, USA, Catalog # M03166)
Host
Rabbit
Contents
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
This TOMM40 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 22-56 amino acids from human TOMM40.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M03166 is reactive to TOMM40 in Human
Applications
M03166 is guaranteed for Flow Cytometry, IF, WB Boster Guarantee
Observed Molecular Weight
Calculated molecular weight
37893 Da
Background of TOMM40
Channel-forming protein essential for import of protein precursors into mitochondria.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
IF: 1:25
WB: 1:2000
FC: 1:25
Validation Images & Assay Conditions
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Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized U-2 OS (human osteosarcoma cell line) cells labeling TOMM40 with M03166 at 1/25 dilution, followed by Dylight® 488-conjugated goat anti-rabbit IgG secondary antibody at 1/200 dilution (green). Immunofluorescence image showing mitochondrion staining on U-2 OS cell line. Cytoplasmic actin is detected with Dylight® 554 Phalloidin at 1/100 dilution (red).The nuclear counter stain is DAPI (blue).
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All lanes : Anti-TOMM40 Antibody (N-Term) at 1:2000 dilution
Lane 1: Human brain lysate
Lane 2: 293 whole cell lysate
Lane 3: U-87 MG whole cell lysate
Lane 4: Hut78 whole cell lysate
Lane 5: Hela whole cell lysate
Lane 6: A431 whole cell lysate
Lane 7: HepG2 whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.
Predicted band size : 38 kDa
Blocking/Dilution buffer: 5% NFDM/TBST.
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Overlay histogram showing HeLa cells stained with M03166 (green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (M03166, 1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed at 1/200 dilution for 40 min at 37ºC. Isotype control antibody (blue line) was rabbit IgG1 (1g/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.
Protein Target Info & Infographic
Gene/Protein Information For TOMM40 (Source: Uniprot.org, NCBI)
Gene Name
TOMM40
Full Name
Mitochondrial import receptor subunit TOM40 homolog
Weight
37893 Da
Superfamily
Tom40 family
Alternative Names
C19orf1mitochondrial import receptor subunit TOM40 homolog; D19S1177E; mitochondrial outer membrane protein; PER-EC1; PEREC1Translocase of outer membrane 40 kDa subunit homolog; Protein Haymaker; TOM40p38.5; translocase of outer mitochondrial membrane 40 homolog (yeast) TOMM40 C19orf1, D19S1177E, PER-EC1, PEREC1, TOM40 translocase of outer mitochondrial membrane 40 mitochondrial import receptor subunit TOM40 homolog|mitochondrial outer membrane protein|p38.5|protein Haymaker|translocase of outer membrane 40 kDa subunit homolog|translocase of outer mitochondrial membrane 40 homolog
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on TOMM40, check out the TOMM40 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for TOMM40: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-TOMM40 Antibody (N-Term) (M03166)
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