Product Info Summary
SKU: | A04143-1 |
---|---|
Size: | 100 μg/vial |
Reactive Species: | Human |
Host: | Rabbit |
Application: | ELISA, Flow Cytometry, IP, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-TOM1 Antibody Picoband™
SKU/Catalog Number
A04143-1
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-TOM1 Antibody Picoband™ catalog # A04143-1. Tested in WB, IHC, ICC/IF, IP, FCM, ELISA applications. This antibody reacts with Human.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-TOM1 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A04143-1)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Immunogen
E.coli-derived human TOM1 recombinant protein (Position: R84-K480). Human TOM1 shares 87.4% amino acid (aa) sequence identity with mouse TOM1.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A04143-1 is reactive to TOM1 in Human
Applications
A04143-1 is guaranteed for ELISA, Flow Cytometry, IP, IF, IHC, ICC, WB Boster Guarantee
Observed Molecular Weight
54 kDa
Calculated molecular weight
54 kDa
Background of TOM1
Target of Myb protein 1 is a protein that in humans is encoded by the TOM1 gene. This gene was identified as a target of the v-myb oncogene. The encoded protein shares its N-terminal domain in common with proteins associated with vesicular trafficking at the endosome. It is recruited to the endosomes by its interaction with endofin. Several alternatively spliced transcript variants encoding different isoforms have been found for this gene.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Assay dilution & Images
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5 μg/ml, Human
Immunohistochemistry, 2-5 μg/ml, Human
Immunocytochemistry/Immunofluorescence, 5 μg/ml, Human
Immunoprecipitation, 2-4 μg/ml, Human
Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human
ELISA, 0.1-0.5 μg/ml, Human
Validation Images & Assay Conditions
![a04143 1 tom1 primary antibodies wb testing 1 a04143 1 tom1 primary antibodies wb testing 1](https://www.bosterbio.com/media/catalog/product/a/0/a04143-1-tom1-primary-antibodies-wb-testing-1.jpg)
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Figure 1. Western blot analysis of TOM1 using anti-TOM1 antibody (A04143-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human K562 whole cell lysates,
Lane 3: human HepG2 whole cell lysates,
Lane 4: human 293T whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TOM1 antigen affinity purified polyclonal antibody (Catalog # A04143-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TOM1 at approximately 54 kDa. The expected band size for TOM1 is at 54 kDa.
![a04143 1 tom1 primary antibodies ihc testing 2 a04143 1 tom1 primary antibodies ihc testing 2](https://www.bosterbio.com/media/catalog/product/a/0/a04143-1-tom1-primary-antibodies-ihc-testing-2.jpg)
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Figure 2. IHC analysis of TOM1 using anti-TOM1 antibody (A04143-1).
TOM1 was detected in a paraffin-embedded section of human thyroid cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-TOM1 Antibody (A04143-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
![a04143 1 tom1 primary antibodies ihc testing 3 a04143 1 tom1 primary antibodies ihc testing 3](https://www.bosterbio.com/media/catalog/product/a/0/a04143-1-tom1-primary-antibodies-ihc-testing-3.jpg)
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Figure 3. IHC analysis of TOM1 using anti-TOM1 antibody (A04143-1).
TOM1 was detected in a paraffin-embedded section of human thyroid cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-TOM1 Antibody (A04143-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
![a04143 1 tom1 primary antibodies if testing 4 a04143 1 tom1 primary antibodies if testing 4](https://www.bosterbio.com/media/catalog/product/a/0/a04143-1-tom1-primary-antibodies-if-testing-4.jpg)
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Figure 4. IF analysis of TOM1 using anti-TOM1 antibody (A04143-1).
TOM1 was detected in an immunocytochemical section of HELA cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-TOM1 Antibody (A04143-1) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1142) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
![a04143 1 tom1 primary antibodies fcm testing 5 a04143 1 tom1 primary antibodies fcm testing 5](https://www.bosterbio.com/media/catalog/product/a/0/a04143-1-tom1-primary-antibodies-fcm-testing-5.jpg)
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Figure 5. Flow Cytometry analysis of Hela cells using anti-TOM1 antibody (A04143-1).
Overlay histogram showing Hela cells stained with A04143-1 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-TOM1 Antibody (A04143-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Protein Target Info & Infographic
Gene/Protein Information For TOM1 (Source: Uniprot.org, NCBI)
Gene Name
TOM1
Full Name
Target of Myb protein 1
Weight
54 kDa
Superfamily
TOM1 family
Alternative Names
ADP-ribosylation factor-binding protein GGA3; golgi associated, gamma adaptin ear containing, ARF binding protein 3; golgi-associated, gamma adaptin ear containing, ARF binding protein 3; KIAA0154Golgi-localized, gamma ear-containing, ARF-binding protein 3 TOM1 target of myb1 membrane trafficking protein target of Myb protein 1|target of myb 1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on TOM1, check out the TOM1 Infographic
![TOM1 infographic](/media/images/gene-infographic-example.jpg)
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for TOM1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-TOM1 Antibody Picoband™ (A04143-1)
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