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Product Info Summary
| SKU: | PB10104 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | ELISA, WB |
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Product info
Product Name
Anti-TNFRSF14 Antibody Picoband®
View all HVEM/TNFRSF14 Antibodies
SKU/Catalog Number
PB10104
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-TNFRSF14 Antibody Picoband® catalog # PB10104. Tested in ELISA, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-TNFRSF14 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB10104)
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E. coli-derived human TNFRSF14/HVEM recombinant protein (Position: L39-V202).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
PB10104 is reactive to TNFRSF14 in Human
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
37 kDa
Calculated molecular weight
30392 MW
Background of HVEM/TNFRSF14
Herpesvirus entry mediator (HVEM), also known as tumor necrosis factor receptor superfamily member 14 (TNFRSF14), is a human cell surface receptor of the TNF-receptor superfamily. The encoded protein functions in signal transduction pathways that activate inflammatory and inhibitory T-cell immune response. It binds herpes simplex virus (HSV) viral envelope glycoprotein D (gD), mediating its entry into cells. Alternative splicing results in multiple transcript variants.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PB10104 is guaranteed for ELISA, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
ELISA, 0.1-0.5μg/ml, -
Western blot, 0.1-0.5μg/ml, Human
Positive Control
WB: HELA whole cell, SW620 whole cell
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of TNFRSF14/HVEM using anti-TNFRSF14/HVEM antibody (PB10104).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: HELA whole cell lysates,
Lane 2: SW620 whole cell lysates lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TNFRSF14/HVEM antigen affinity purified polyclonal antibody (Catalog # PB10104) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TNFRSF14/HVEM at approximately 37 kDa. The expected band size for TNFRSF14/HVEM is at 30 kDa.
Protein Target Info & Infographic
Gene/Protein Information For TNFRSF14 (Source: Uniprot.org, NCBI)
Gene Name
TNFRSF14
Full Name
Tumor necrosis factor receptor superfamily member 14
Weight
30392 MW
Superfamily
tumor necrosis factor receptor superfamily
Alternative Names
Tumor necrosis factor receptor superfamily member 14;Herpes virus entry mediator A;Herpesvirus entry mediator A;HveA;Tumor necrosis factor receptor-like 2;TR2;CD270;TNFRSF14;HVEA, HVEM;UNQ329/PRO509; TNFRSF14 ATAR, CD270, HVEA, HVEM, LIGHTR, TR2 TNF receptor superfamily member 14 tumor necrosis factor receptor superfamily member 14|CD40-like protein|herpes virus entry mediator A|tumor necrosis factor receptor superfamily, member 14 (herpesvirus entry mediator)|tumor necrosis factor receptor-like gene2
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on TNFRSF14, check out the TNFRSF14 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for TNFRSF14: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-TNFRSF14 Antibody Picoband® (PB10104)
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Customer Q&As
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1 Customer Q&As for Anti-TNFRSF14 Antibody Picoband®
Question
We are currently using anti-TNFRSF14 antibody PB10104 for human tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human. Is it true that the antibody can work on canine tissues as well?
Verified Customer
Verified customer
Asked: 2019-07-30
Answer
The anti-TNFRSF14 antibody (PB10104) has not been validated for cross reactivity specifically with canine tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in canine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-07-30
