Product Info Summary
SKU: | A09304 |
---|---|
Size: | 100ul |
Reactive Species: | Human, Rat |
Host: | Rabbit |
Application: | ELISA, IHC-P, WB, IF (paraffin) |
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Product info
Product Name
Anti-TMBIM4/Gaap Antibody
SKU/Catalog Number
A09304
Size
100ul
Form
Liquid
Description
Boster Bio Anti-TMBIM4/Gaap Antibody (Catalog# A09304). Tested in WB, IHC-P, IF (paraffin), ELISA applications. This antibody reacts with Human, Rat.
Storage & Handling
Store at -20°C for one year. For short-term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-TMBIM4/Gaap Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A09304)
Host
Rabbit
Contents
PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A peptide derived from human TMBIM4. Immunogen sequence location: 121 - 170
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A09304 is reactive to TMBIM4 in Human, Rat
Reconstitution
Observed Molecular Weight
72 kDa
Calculated molecular weight
26971 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A09304 is guaranteed for ELISA, IHC-P, WB, IF (paraffin) Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western Blot, 1:500 - 1:2000
Immunohistochemistry, 1:100 - 1:300
ELISA, 1:5000
Not yet tested in other applications.
Validation Images & Assay Conditions
Click image to see more details
Western blot analysis of lysates from HepG2 cells, using TMBIM4 Antibody. The lane on the right is blocked with the synthesized peptide.
Protein Target Info & Infographic
Gene/Protein Information For TMBIM4 (Source: Uniprot.org, NCBI)
Gene Name
TMBIM4
Full Name
Protein lifeguard 4
Weight
26971 MW
Superfamily
BI1 family
Alternative Names
TMBIM4;GAAP;LFG4;CGI-119;Protein lifeguard 4;Golgi anti-apoptotic protein;Protein S1R;Transmembrane BAX inhibitor motif-containing protein 4;Z-protein TMBIM4 CGI-119, GAAP, LFG4, S1R, ZPRO transmembrane BAX inhibitor motif containing 4 protein lifeguard 4|Golgi anti-apoptotic protein|Z-protein|transmembrane BAX inhibitor motif-containing protein 4
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on TMBIM4, check out the TMBIM4 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for TMBIM4: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-TMBIM4/Gaap Antibody (A09304)
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No publications found for A09304
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Customer Reviews
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Customer Q&As
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6 Customer Q&As for Anti-TMBIM4/Gaap Antibody
Question
My question regarding product A09304, anti-TMBIM4/Gaap antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2020-04-14
Answer
We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A09304 anti-TMBIM4/Gaap antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2020-04-14
Question
Is a blocking peptide available for product anti-TMBIM4/Gaap antibody (A09304)?
Verified Customer
Verified customer
Asked: 2019-12-13
Answer
We do provide the blocking peptide for product anti-TMBIM4/Gaap antibody (A09304). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2019-12-13
Question
We are currently using anti-TMBIM4/Gaap antibody A09304 for human tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human, rat. Is it possible that the antibody can work on primate tissues as well?
Verified Customer
Verified customer
Asked: 2019-12-02
Answer
The anti-TMBIM4/Gaap antibody (A09304) has not been tested for cross reactivity specifically with primate tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in primate you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-12-02
Question
See below the WB image, lot number and protocol we used for adrenal gland using anti-TMBIM4/Gaap antibody A09304. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2019-12-02
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-12-02
Question
I was wanting to use to test anti-TMBIM4/Gaap antibody A09304 on rat adrenal gland for research purposes, then I may be interested in using anti-TMBIM4/Gaap antibody A09304 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2017-12-26
Answer
The products we sell, including anti-TMBIM4/Gaap antibody A09304, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2017-12-26
Question
Would anti-TMBIM4/Gaap antibody A09304 work for WB with adrenal gland?
G. Li
Verified customer
Asked: 2013-03-13
Answer
According to the expression profile of adrenal gland, TMBIM4 is highly expressed in adrenal gland. So, it is likely that anti-TMBIM4/Gaap antibody A09304 will work for WB with adrenal gland.
Boster Scientific Support
Answered: 2013-03-13